Major polyadenylated transcripts of cassava latent virus and location of the gene encoding coat protein

Abstract

The nucleotide sequences of infectious cloned DNAs 1 and 2 of a Kenyan isolate of cassava latent virus (CLV) have been determined. Five virus‐specific polyadenylated transcripts have been identified and mapped either to the viral or complementary sense DNAs of both components of the CLV genome, confirming that transcription is bidirectional on both DNAs. A major mRNA has been translated in vitro to yield a 30 000 mol. wt. product, which is precipitated by antibodies raised against whole virus, and has been mapped by both the S1 nuclease procedure and hybrid‐arrested translation to the long open reading frame (ORF) in the viral sense of DNA 1 which encodes the coat protein. Other transcripts were of sufficient size and appropriate origin to encode at least five potential products.

Bidirectional transcription of maize streak virus DNA and identification of the coat protein gene

“…Five transcripts have been mapped to either the viral or complementary senses of CLV DNA (8). For MSV we report the detection and mapping of three transcripts.…”

“…The primary nucleotide sequence of the genomes of four members of the geminivirus group have been determined, namely cassava latent virus Nucleic Acids Research the gene encoding the virus coat protein determined (8).…”

Lethal Mutations Within the Conserved Stem-loop of African Cassava Mosaic Virus DNA are Rapidly Corrected by Genomic Recombination

“…Two genomic components (DNAs A and B) are essential for systemic infection (Stanley, 1983;Stanley & Gay, 1983) and genetic analysis has revealed that DNA A is responsible for viral DNA replication, coat protein synthesis and the production of virus particles (Townsend et al, 1985(Townsend et al, , 1986Klinkenberg & Stanley, 1990) while DNA B is required for cell-to-cell and systemic movement of virus (von Arnim et al, 1993). The genomic components have a highly conserved sequence of approximately 200 nucleotides (common region) that is located within their intergenic regions (Stanley & Gay, 1983).…”

Recombination between viral DNA and the transgenic coat protein gene of African cassava mosaic geminivirus.

“…Proteins were transferred to nitrocellulose (Schleicher and Schuell) using a Bio-Rad semi-dry trans-blot assembly. The presence of coat protein was detected using polyclonal antiserum raised against purified ACMV (Townsend et al, 1985). Blots were developed using alkaline phosphatase-conjugated goat anti-rabbit IgG (Sigma).…”