Recombination between viral DNA and the transgenic coat protein gene of African cassava mosaic geminivirus.

Frischmuth, Thomas; Stanley, John

doi:10.1099/0022-1317-79-5-1265

Cited by 49 publications

(25 citation statements)

References 23 publications

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“…Thus, Rep-mediated initiation, termination of replication at two different transgenic origins, and circularization of the nascent strand will produce a replicon containing a single intact copy of the origin. A previous investigation with a similar construct based on an ACMV DNA-A deletion mutant with flanking origins of replication failed to mobilize a 1,900-bp replicon in this way (14). The reason for this different behaviour is unclear but presumably resides in the difference in composition or size of the transgenes that could affect the mobilization, replication, and/or stability of the replicons.…”

Section: Discussionmentioning

confidence: 95%

Novel System for the Simultaneous Analysis ofGeminivirus DNA Replication and Plant Interactions inNicotianabenthamiana

Hong

Stanley

Wezel

2003

J Virol

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The origin of replication of African cassava mosaic virus (ACMV) and a gene expression vector based on Potato virus X were exploited to devise an in planta system for functional analysis of the geminivirus replicationassociated protein (Rep) in transgenic Nicotiana benthamiana line pOri-2. This line contains an integrated copy of a tandem repeat of the ACMV origin of replication flanking nonviral sequences that can be mobilized and replicated by Rep as an episomal replicon. A Rep-GFP fusion protein can also mobilize and amplify the replicon, facilitating Rep detection in planta. The activity of Rep and its mutants, Rep-mediated host response, and the correlation between Rep intracellular localization and biological functions could be effectively assessed by using this in planta system. Our results indicate that modification of amino acid residues R 2 , R 5 , R 7 and K 11 or H 56 , L 57 and H 58 prevent Rep function in replication. This defect correlates with possible loss of Rep nuclear localization and inability to trigger the host defense mechanism resembling a hypersensitive response.

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Section: Discussionmentioning

confidence: 95%

Novel System for the Simultaneous Analysis ofGeminivirus DNA Replication and Plant Interactions inNicotianabenthamiana

Hong

Stanley

Wezel

2003

J Virol

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“…Features such as failure in gap repair, extended lifetime of open circular DNA (pararetrovirus episomal particles), or recombination between free ends of "virus" and plant DNA have been suggested by Jakowitsch et al (1999) as facilitators of integration. When a pararetrovirus (Cauliflower mosaic virus, CaMV) deficient in gene function is introduced into a plant containing a translational transactivator transgene, the virus can utilise the gene product to complete the infection cycle (Kirá ly et al, 1998), a similar phenomenon to that was seen with geminiviruses (Frischmuth and Stanley, 1998).…”

Section: Discussionmentioning

confidence: 97%

Characterisation of pararetrovirus-like sequences in the genome of potato <i>(Solanum tuberosum)</i>

Hansen

Harper²,

Heslop-Harrison³

2005

Cytogenet Genome Res

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Three families of pararetrovirus-like sequences were isolated from the genome of potato using PCR of a characteristic fragment extending from the end of the transactivator domain. The potato pararetrovirus-like sequences are abundant in the nuclear genome of potato as demonstrated by their hybridisation to high-molecular weight DNA in Southern transfers and by fluorescence in situ hybridisation. Sequencing of cloned PCR products demonstrated that the potato pararetrovirus-like sequences were similar to other pararetroviral sequences and also to some expressed sequences from tobacco and tomato, notably from callus and Agrobacterium-infected tissue. It is possible that the potato pararetroviral sequences defend against viral genes via silencing mechanisms, although, as in Petunia or banana, their transcription and recombination may cause infection under stress conditions.

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“…Several examples of recombination between a virus and its homologous transgene have been reported (Adair and Kearney, 2000;Borja et al, 1999;Frischmuth and Stanley, 1998;Gal et al, 1992Gal et al, , 1996Allison, 1994, 1996;Schoelz and Wintermantel, 1993;Turturo et al, 2008;Wintermantel and Schoelz, 1996). In most cases, a defective virus was used such that recombination would restore full infectivity and thus confer a significant selective advantage on the recombinant.…”

Section: Hgt From Gm Plants To Virusesmentioning

confidence: 99%

Risks from GMOs due to Horizontal Gene Transfer

Keese¹

2008

Environ. Biosafety Res.

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Horizontal gene transfer (HGT) is the stable transfer of genetic material from one organism to another without reproduction or human intervention. Transfer occurs by the passage of donor genetic material across cellular boundaries, followed by heritable incorporation to the genome of the recipient organism. In addition to conjugation, transformation and transduction, other diverse mechanisms of DNA and RNA uptake occur in nature. The genome of almost every organism reveals the footprint of many ancient HGT events. Most commonly, HGT involves the transmission of genes on viruses or mobile genetic elements. HGT first became an issue of public concern in the 1970s through the natural spread of antibiotic resistance genes amongst pathogenic bacteria, and more recently with commercial production of genetically modified (GM) crops. However, the frequency of HGT from plants to other eukaryotes or prokaryotes is extremely low. The frequency of HGT to viruses is potentially greater, but is restricted by stringent selection pressures. In most cases the occurrence of HGT from GM crops to other organisms is expected to be lower than background rates. Therefore, HGT from GM plants poses negligible risks to human health or the environment.

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Recombination between viral DNA and the transgenic coat protein gene of African cassava mosaic geminivirus.

Cited by 49 publications

References 23 publications

Novel System for the Simultaneous Analysis ofGeminivirus DNA Replication and Plant Interactions inNicotianabenthamiana

Novel System for the Simultaneous Analysis ofGeminivirus DNA Replication and Plant Interactions inNicotianabenthamiana

Characterisation of pararetrovirus-like sequences in the genome of potato <i>(Solanum tuberosum)</i>

Risks from GMOs due to Horizontal Gene Transfer

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