Major histocompatibility complex (MHC) restriction of foreign transplantation antigens in rats rendered tolerant at birth.

Abstract

When mice and rats are inoculated at birth with MHC-incompatible bone marrow cells (BMC),' they not only are rendered immunologically tolerant of the foreign transplantation antigens present on the donor cells, they also become unresponsive to donor strain skin grafts known to possess skin-specific (Skn) antigens (1) . To account for this situation we have proposed that MHC restriction accompanies the induction of tolerance, i .e ., because donor cells migrate to the thymus, their T cell repertoire is restricted by the host's MHC (1) . We now present further evidence that this is the case by demonstrating that third party skin grafts survive significantly longer on tolerant rats if they are MHC compatible with the tolerance-inducing inoculum, than if they are MHC compatible with the host .

Prevention of Graft-Versus-Host Diseases by in Vivo supCD28mAb-Expanded Antigen-Specific nTreg Cells

“…5A). Briefly, 1 × 10 8 /150 μl (Lewis x DA) F1 bone morrow cells were injected into the neonatal DA rats (6, 11) and, 4 weeks after, the characterization was performed by an FCM analysis using a specific antibody (clone I1–69) that recognized the Lewis MHC class I molecules (Fig. 5B).…”

“…Tregs specifically express the transcription factor Foxp3 , which appears to act as a master control gene for their development and function (3, 5, 6). The forced expression of the Foxp3 gene can convert the murine naive T cells to nonnatural Tregs, which phenotypically and functionally resemble the natural Tregs (5).…”