Major Changes in Plastid Protein Import and the Origin of the Chloroplastida

Knopp, Michael; Garg, Sriram G.; Handrich, Maria; Gould, Sven B.

doi:10.1016/j.isci.2020.100896

Cited by 23 publications

(24 citation statements)

References 109 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, the chloroplast outer envelope pore Toc75 is thought to derive from Omp85, a bacterial chaperone that aids assembly of certain outer membrane proteins recognized by carboxyl-terminal stretches ending in phenylalanine [ 24 ]. Toc75 switched polarity to accept polypeptides from the host cytoplasm rather than the bacterial periplasm [ 25 ], engendering a requirement for phenylalanine at the cTP N-terminus ( Fig 1 ) that endures in basal algal lineages [ 26 ]. Also, the chloroplast inner envelope pore is most likely formed by Tic20/21, for which homologues have been identified in cyanobacteria [ 27 ], and the mitochondrial outer membrane pore Tom40 probably arose de novo within the proto-mitochondrion [ 28 ].…”

Section: From Antagonism To Endosymbiosis: Evolving Inside Outmentioning

confidence: 99%

The role of antimicrobial peptides in the evolution of endosymbiotic protein import

Caspari

Lafontaine

2021

PLoS Pathog

View full text Add to dashboard Cite

Section: From Antagonism To Endosymbiosis: Evolving Inside Outmentioning

confidence: 99%

The role of antimicrobial peptides in the evolution of endosymbiotic protein import

Caspari

Lafontaine

2021

PLoS Pathog

View full text Add to dashboard Cite

“…Since Rhodophyte and Glaucophyte cTPs start with a conserved phenylalanine [92], chloroplast protein import could have benefited from a functional inversion of this cyanobacterial protein in the evolution of the TOC complex. Although this observation was taken as an argument against the emergence of cTPs from HA-RAMPs [93], we argue that HA-RAMPs would have originally interacted with the outer membrane lipid headgroups [5], then crossed the outer membrane spontaneously [94,95], with some HA-RAMPs also interacting with Omp85 proteins [96]. In this view, the most likely evolutionary scenario for chloroplast import has involved recruitment of Omp85 to improve delivery of HA-RAMP-tagged proteins to the import-and-destroy receptor at the inner membrane surface.…”

Section: Targeting Peptides and The Translocation Machinerymentioning

confidence: 99%

Evidence Supporting an Antimicrobial Origin of Targeting Peptides to Endosymbiotic Organelles

Garrido

Caspari

Choquet

et al. 2020

Cells

View full text Add to dashboard Cite

Mitochondria and chloroplasts emerged from primary endosymbiosis. Most proteins of the endosymbiont were subsequently expressed in the nucleo-cytosol of the host and organelle-targeted via the acquisition of N-terminal presequences, whose evolutionary origin remains enigmatic. Using a quantitative assessment of their physico-chemical properties, we show that organelle targeting peptides, which are distinct from signal peptides targeting other subcellular compartments, group with a subset of antimicrobial peptides. We demonstrate that extant antimicrobial peptides target a fluorescent reporter to either the mitochondria or the chloroplast in the green alga Chlamydomonas reinhardtii and, conversely, that extant targeting peptides still display antimicrobial activity. Thus, we provide strong computational and functional evidence for an evolutionary link between organelle-targeting and antimicrobial peptides. Our results support the view that resistance of bacterial progenitors of organelles to the attack of host antimicrobial peptides has been instrumental in eukaryogenesis and in the emergence of photosynthetic eukaryotes.

show abstract

“…The vast majority of plastid proteins are encoded by the nuclear genome and, after their synthesis in the cytosol, are imported into the plastid by the TOC/TIC (translocon of the outer/inner envelope of the chloroplast) complex (Richardson & Schnell 2020). Embryophytes have evolved the most sophisticated TOC/TIC complexes (Knopp et al, 2020) and our data confirm that the hornwort TOC complex is comprised of the same key proteins that are found in other embryophytes, mainly TOC75, TOC34 and TOC159 (Richardson & Schnell 2020) (Figure 2A and supplementary figures S7-19). The recycling of major TOC components is regulated by the RING-type ubiquitin E3 ligase SP1, which targets these proteins for proteasomal degradation (Ling et al 2012) (Figure 2B).…”

Section: Results and Discussion Full Conservation Of Toc But Only Partial Conservation Of Tic In Hornwort Chloroplastsmentioning

confidence: 99%

Loss of plastid developmental genes coincides with a reversion to monoplastidy in hornworts

MacLeod

Raval

Stockhorst³

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

The first plastid evolved from an endosymbiotic cyanobacterium in the common ancestor of the Archaeplastida. The transformative steps from cyanobacterium to organelle included the transfer of control over developmental processes; a necessity for the host to orchestrate, for example, the fission of the organelle. The plastids of almost all embryophytes divide independent from nuclear division, leading to cells housing multiple plastids. Hornworts, however, are monoplastidic (or near-monoplastidic) and their photosynthetic organelles are a curious exception among embryophytes for reasons such as the occasional presence of pyrenoids. Here we screened genomic and transcriptomic data of eleven hornworts for components of plastid developmental pathways. We find intriguing differences among hornworts and specifically highlight that pathway components involved in regulating plastid development and biogenesis were differentially lost in this group of bryophytes. In combination with ancestral state reconstruction, our data suggest that hornworts have reverted back to a monoplastidic phenotype due to the combined loss of two plastid division-associated genes: ARC3 and FtsZ2.

show abstract

Major Changes in Plastid Protein Import and the Origin of the Chloroplastida

Cited by 23 publications

References 109 publications

The role of antimicrobial peptides in the evolution of endosymbiotic protein import

The role of antimicrobial peptides in the evolution of endosymbiotic protein import

Evidence Supporting an Antimicrobial Origin of Targeting Peptides to Endosymbiotic Organelles

Loss of plastid developmental genes coincides with a reversion to monoplastidy in hornworts

Contact Info

Product

Resources

About