Maize DNA Polymerase 2 is a Phosphoprotein with Increasing Activity During Germination

Coello, Patricia; Vázquez‐Ramos, Jorge M.

doi:10.1111/j.1432-1033.1995.0099f.x

Cited by 22 publications

(25 citation statements)

References 36 publications

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“…A certain amount of PCNA protein is present in dry seeds, as we have reported for other cell cycle-related proteins and activities in maize dry seeds, such as replicative-type DNA polymerases and DNA primase (Coello andVázquez-Ramos 1995, García et al 1997), DNA ligase , putative cyclin B, cyclin D, Cdk4 kinase, E2F and p53 proteins (Cruz- García et al 1998) and p34 cdc2 kinase (Herrera-Teigeiro et al 1999). It would appear that during the seed development program, there might be a decision to store a potential amount of cell cycle proteins that will be required and essential for the germination process.…”

Section: Discussionsupporting

confidence: 68%

Proliferating cell nuclear antigen expression in maize seed development and germination: Regulation by phytohormones and its association with putative cell cycle proteins

Herrera¹,

Sánchez²,

Molina³

et al. 2000

Physiologia Plantarum

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The proliferating cell nuclear antigen (PCNA) plays a fundamental role in DNA replication and repair and recently, it has been found associated to proteins that control the G1 phase of the cell cycle, such as cyclin D. Maize PCNA cDNA has been cloned and overexpressed in order to raise antibodies. The expression of PCNA has been followed during seed development and seed germination using the homologous antibodies. The protein was found at a constant level during seed development up to 48 days after pollination (DAP) and then the amount declined to very low levels, similar to those found in dry seeds. Upon germination, PCNA levels rose gradually reaching a peak by 20 h germination. Imbibition in the presence of cytokinins (Benzyladenine, BA) produced a sharp increase in amount during the first 3–6 h germination, whereas imbibition in the presence of abscisic acid (ABA) did not alter the pattern of expression as compared with control seeds. Immunoprecipitation experiments showed that PCNA was associated to a putative cyclin D protein during germination and this association was altered by phytohormones. While the complex PCNA‐cyclin D‐like protein was present along the first 15 h of germination under control conditions, it was dissociated after 6 h if embryo axes germinated in the presence of BA or ABA. However, complex dissociation in the presence of BA was due to degradation of the putative cyclin D protein while in the presence of ABA the putative cyclin D was still present. These results are discussed in the context of seed germination and the cell cycle.

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Section: Discussionsupporting

confidence: 68%

Proliferating cell nuclear antigen expression in maize seed development and germination: Regulation by phytohormones and its association with putative cell cycle proteins

Herrera¹,

Sánchez²,

Molina³

et al. 2000

Physiologia Plantarum

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“…merases which have been identified in several plant species such as wheat [39][40][41][42], maize [43][44][45][46] and pea [47]. A challenge for the future is to identify which DNA polymerase activities act during this stage of the geminiviral DNA replication cycle.…”

Section: Review Articlementioning

confidence: 99%

Geminivirus DNA replication

Gutiérrez¹

1999

Cellular and Molecular Life Sciences (CMLS)

253

160

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Geminiviruses are DNA viruses which infect plants. They have a small genome and encode only a few proteins. Therefore, their DNA replication cycle relies largely on the use of cellular DNA replication proteins. The strategy used by geminiviruses to replicate their single-stranded DNA (ssDNA) genome consists of a first stage of conversion of ssDNA into double-stranded DNA (dsDNA) intermediates and, then, the use of dsDNA as a template to amplify viral dsDNA and to produce mature ssDNA genomes by a rolling-circle replication mechanism. In addition, the accumulating evidence indicates that viral DNA replication is somehow coupled to the cell cycle regulatory network of the infected cell. For these reasons, geminiviruses are excellent model systems to understand the regulation of DNA replication and cell cycle in plant cells. Recent years have witnessed significant progress in the identification of cis-acting signals and their interaction with trans-acting factors that contribute to geminivirus origin function. These and other aspects of the geminivirus DNA replication cycle will be reviewed.

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“…DNA pol 1 has a native molecular mass of 350 kDa, prefers poly‐dA/oligo dT to activated DNA or poly‐rA/oligo dT and its activity is stimulated by maize PCNA (García et al 1997). DNA pol 2 has a native molecular mass around 400 kDa, its activity increases importantly during maize germination (Coello et al 1992), appears to be regulated by phosphorylation and it is a low processivity enzyme (Coello and Vázquez‐Ramos 1995a, 1995b) and contains a strongly associated DNA primase activity (García et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Expression of a maize δ‐type DNA polymerase during seed germination

García¹,

Quiroz²,

Uchiyama

et al. 2006

Physiologia Plantarum

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We have cloned a 1563-bp cDNA sequence from a reported 2072-bp cDNA (including a fragment of the 3 0 UTR region, accession number AY109773) corresponding to the carboxy half of maize DNA polymerase d [Zea mays delta-type DNA polymerase catalytic subunit (Zmpold); EC 2.7.7.7], and its sequence shows an identity of 95, 77 and 74% to rice, soybean and Arabidopsis enzymes, respectively, although identity is even higher if only the pold-defining domain sequences are considered. An important difference between the monocot and dicot enzymes is the presence in the former of Zn fingers apparently required for binding to the DNA pold holoenzyme B subunit, which is absent in the dicot enzymes. Expression of Zmpold and protein levels during germination remain unchanged; however, pold shows two peaks of activity, one during early germination, perhaps related to DNA repair processes and a second peak when DNA replication is already in progress, indicating that Zmpold is regulated at the post-translational level. Zmpold has been found mainly in proliferative tissues in seeds and plantlets, although surprisingly, it is also present in seed endosperm, together with Zm proliferating cell nuclear antigen.

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Maize DNA Polymerase 2 is a Phosphoprotein with Increasing Activity During Germination

Cited by 22 publications

References 36 publications

Proliferating cell nuclear antigen expression in maize seed development and germination: Regulation by phytohormones and its association with putative cell cycle proteins

Proliferating cell nuclear antigen expression in maize seed development and germination: Regulation by phytohormones and its association with putative cell cycle proteins

Geminivirus DNA replication

Expression of a maize δ‐type DNA polymerase during seed germination

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