Maintenance of <i>Helicobacter pylori</i> Cultures in Agar Stabs

Xu, Jinghua; Czinn, Steven J.; Blanchard, Thomas G.

doi:10.1111/j.1523-5378.2010.00769.x

Cited by 6 publications

(4 citation statements)

References 18 publications

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“…Frequent passage, occurring every several days or at least once per week, also increases the likelihood of introducing airborne molds and other contaminants to the cultures. Some of these problems can be circumvented by preparing agar stabs for the long term storage of H. pylori or H. felis at 37°C (Xu et al 2010. Visible growth can be observed in these agar stab cultures within several days of inoculation and will remain viable for up to a month in the case of H. felis, or for at least several months in the case of H. pylori.…”

Section: Basic Protocol 3 Culture Of Helicobacter Organisms In Agar Smentioning

confidence: 99%

Laboratory Maintenance of Helicobacter Species

Blanchard

Nedrud

2012

CP Microbiology

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Helicobacter species are Gram‐negative bacteria that colonize the gastric or intestinal mucosa of many mammalian and avian hosts and induce histologic inflammation. The association of H. pylori with gastritis, peptic ulcer disease, and gastric cancers makes it a significant human pathogen. Animal models for these diseases are being used to explore the pathogenesis of H. pylori infection and in vaccine development. Both bacterial and host factors contribute to Helicobacter pathogenesis; therefore, the microbiology is very important. This unit describes how to culture the most commonly used gastric Helicobacter species, H. pylori, H. mustelae, and H. felis. Curr. Protoc. Microbiol. 24:8B.1.1‐8B.1.19. © 2012 by John Wiley & Sons, Inc.

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Section: Basic Protocol 3 Culture Of Helicobacter Organisms In Agar Smentioning

confidence: 99%

Laboratory Maintenance of Helicobacter Species

Blanchard

Nedrud

2012

CP Microbiology

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“…In a study conducted by Lee et al (29) after comparing some culture methods from biopsy samples, they determined that the culture media, Thayer agar, Brucella agar, Chocolate agar, and Brain heart infusion agar acted in the same way, obtaining similar results under conditions of 10% CO 2 and 96% humidity at 37°C. Similarly, in a work carried out by Xu et al (30) it was determined that in Brucella agar, chocolate agar and brain heart infusion, H. pylori agar has the ability to live up to 56 days.…”

Section: Discussionmentioning

confidence: 85%

Detection of Helicobacter pylori from Human Biological Samples (Feces) by Antigenic Screening and Culture

Guaman

Bayas-Morejón

Arcos

et al. 2018

Jundishapur J Microbiol

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Background: This research addressed the topic of timely diagnosis of Helicobacter pylori that is currently a problem for public health in Ecuador and worldwide. The prevalence of the pathogen has increased in the last decade, especially due to its direct participation in gastric cancer generation. Objectives: The present investigation was carried out to detect H. pylori in human stool samples by antigenic screening and culture. Methods: The methodology used in this study was direct and indirect analysis of patients with H. pylori infection symptoms. Overall, 50 stool samples were collected (16 from males and 34 from females) and transferred immediately to the laboratory; patients were excluded due to causes such as contamination or insufficient amounts of sample for the analysis. From these samples, antigenic screening and culture in plates were carried out using the Brucella blood agar selective medium under controlled conditions. The two methods were analysed using the Kappa coefficient. Results: After antigenic screening analysis, 25 samples (50%) were positive for H. pylori with four false positives for both genders. The culture results showed that 19 samples (38%) were positive with 32 isolates characteristic for H. pylori. After agreement analysis, the kappa index was 0.28 (discrete). Conclusions:The detection rate by antigenic screening was higher than that obtained by culture, this is mainly due to the lack of viable cells and the difficulty of adapting adequate isolation techniques.

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“…Other information gathered this year concerns the possibility to maintain viable H. pylori grown in agar stabs for prolonged periods of time (56 days) when a temperature of 37 °C with 10% CO 2 atmosphere was used whereas the bacteria did not survive at room temperature [29].…”

Section: Invasive Testsmentioning

confidence: 99%

Diagnosis of Helicobacter pylori Infection

2011

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While few new methods have been proposed for Helicobacter pylori diagnosis, there are still a number of articles evaluating the current methods and trying to improve their accuracy. Invasive tests EndoscopyAttempts to diagnose Helicobacter pylori infection directly during endoscopy have been made in the past. While the observation of H. pylori per se is usually not possible, indirect evidence of its presence can be found. This year, using standard endoscopy in children, Hidaka et al. [1] were able to show that the absence of regular arrangement of collecting venules at two sites, indicated the absence of H. pylori infection with an excellent sensitivity (100%) and specificity (90%). The respective values for antral nodularity, the usual criterion, were 84% and 100%.Three studies evaluated narrow band imaging (NBI), a technique enhancing the mucosal and capillary patterns of the gastric surface. Three hundred patients were explored with conventional NBI and 5 mucosal patterns were identified, corresponding to different grades of histological gastritis [2]. Magnifying NBI was also used to investigate the changes in gastric mucosal patterns before and 12 weeks after H. pylori eradication. In patients without severe atrophy and intestinal metaplasia (IM), the changes in pit size and density of fine irregular vessels were observed with a perfect sensitivity and specificity. In contrast, no change occurred in patients with severe atrophy and IM, regardless of H. pylori eradication [3]. The authors emphasized that this method predicts histological conditions and pepsinogen levels and may be cost effective in gastric cancer surveillance [4].Confocal laser endomicroscopy, a magnifying endoscopy technique, allows an in depth analysis of the gastric mucosa. In a first study, Ji et al. [5] identified H. pylori gastritis with a 92% accuracy. The mean kappa value for inter-observer agreement was 0.78. In a second study, the same group focused on the severity of H. pylori-associated gastritis, especially atrophy and IM, with a rather good diagnostic accuracy [6]. HistologyThe ever well-known OLGA (Operative Link on Gastritis Assessment) staging system was highlighted again under the aspect of histology reporting of gastritis. It is especially valuable as it allows a prediction of the gastric cancer risk [7]. A long-term follow-up (12 years) of 93 patients confirmed that all invasive or intra-epithelial gastric neoplasia were consistently associated with high-risk (III ⁄ IV) OLGA stages [8].Immunohistochemistry can be used to assess the presence of H. pylori with more certainty. However, its systematic use in the routine of a pathology laboratory does not seem necessary because it is of no value in cases with an absence of pathological abnormality nor reactive gastropathy [6]. However, it allows the AbstractWhen an endoscopy is performed, it now becomes easier to observe indirect evidence of the presence of a Helicobacter pylori infection, given the progress of new methods including magnifying narrow band imaging or confocal l...

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Maintenance of Helicobacter pylori Cultures in Agar Stabs

Cited by 6 publications

References 18 publications

Laboratory Maintenance of Helicobacter Species

Laboratory Maintenance of Helicobacter Species

Detection of Helicobacter pylori from Human Biological Samples (Feces) by Antigenic Screening and Culture

Diagnosis of Helicobacter pylori Infection

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