Laboratory Maintenance of <i>Helicobacter</i> Species

Blanchard, Thomas G.; Nedrud, John G.

doi:10.1002/9780471729259.mc08b01s24

Cited by 40 publications

(34 citation statements)

References 20 publications

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“…In general, our results are in agreement with a recent review that describes laboratory procedures for culture and maintenance of Helicobacter species, where it is emphasized the use of nutrient‐rich agars such us Columbia or Brucella for growing this fastidious organism (Blanchard and Nedrud, ; Posteraro, ).…”

Section: Resultssupporting

confidence: 91%

Comparison of media and growth conditions for culturing enterohepaticHelicobacterspecies

Ochoa

Martínez

Fernández

et al. 2019

Lett Appl Microbiol

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This research aims to compare the culturing conditions for enterohepatic Helicobacter, evaluating culture media, incubation atmosphere and susceptibility to antimicrobials used to generate selective conditions. Four common media for the closely related genus Campylobacter (Columbia, Bolton, Brucella and CCDA agar), as well as the need for hydrogen in the microaerobic incubation atmosphere, were evaluated. Serial dilutions of 13 strains belonging to six species (H. apodemus, H. bilis, H. canicola, H. canis, H. equorum and Helicobacter sp.) were inoculated in each media and incubated at 37°C for 48 to 96 h using CampyGen (OXOID) and gaseous exchange (including hydrogen) in parallel. Columbia or Brucella agars were the most appropriate for culturing EHH (P < 0·05). However, there was no significant difference between the atmospheres evaluated (P = 0·13). In addition, minimal inhibitory concentration for six antibiotics showed that all isolates were resistant to trimethoprim, whereas for the rest of the antibiotics (cephalothin, cefoperazone, cefsulodin, teicoplanin and vancomycin) the inhibition range was between 8 and 64 μg ml−1. Our findings suggest that Columbia or Brucella media, regardless of the use of hydrogen, can be used for the EHH isolation. In addition, the concentration of antibiotics included in commercial campylobacteria supplements is suitable for EHH species recovery. Significance and Impact of the Study Enterohepatic Helicobacter (EHH) infections have been associated with several diseases in humans such as acute gastroenteritis, inflammatory bowel disease and hepatobiliary diseases. Although they are frequently detected in clinical samples by molecular methods, only occasionally they are isolated using culture conditions described for the taxonomic related pathogen Campylobacter sp. This is because the optimal conditions for the isolation of EHH have not yet been described, which results in an underestimation of the prevalence and clinical importance of these emerging pathogens. Therefore, this study provides insight for culturing EHH species.

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Section: Resultssupporting

confidence: 91%

Comparison of media and growth conditions for culturing enterohepaticHelicobacterspecies

Ochoa

Martínez

Fernández

et al. 2019

Lett Appl Microbiol

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“…The routine cultivation of a number of Helicobacter species (including H. pylori) has been detailed in a number of recent method-based publications (Blanchard and Nedrud 2012;Whitmire and Merrell 2012). These methods however would not be suitable for the more fastidious helicobacters such as H. bizzozeronii, H. salomonis, H. suis and H. heilmannii.…”

Section: Growth Conditionsmentioning

confidence: 99%

The Family Helicobacteraceae

et al. 2014

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“…The genus Helicobacter consists of Gram-negative bacterial species that live in the gastrointestinal tracts of diverse animal hosts [1]–[3]. H. pylori , the best known of these species, chronically infects the gastric (stomach) mucosa of billions of people worldwide, is a major cause of peptic ulcer disease and gastric cancer, and is very diverse genetically.…”

Section: Introductionmentioning

confidence: 99%

Sequence Divergence and Conservation in Genomes of Helicobacter cetorum Strains from a Dolphin and a Whale

2013

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Background and ObjectivesStrains of Helicobacter cetorum have been cultured from several marine mammals and have been found to be closely related in 16 S rDNA sequence to the human gastric pathogen H. pylori, but their genomes were not characterized further.MethodsThe genomes of H. cetorum strains from a dolphin and a whale were sequenced completely using 454 technology and PCR and capillary sequencing.ResultsThese genomes are 1.8 and 1.95 mb in size, some 7–26% larger than H. pylori genomes, and differ markedly from one another in gene content, and sequences and arrangements of shared genes. However, each strain is more related overall to H. pylori and its descendant H. acinonychis than to other known species. These H. cetorum strains lack cag pathogenicity islands, but contain novel alleles of the virulence-associated vacuolating cytotoxin (vacA) gene. Of particular note are (i) an extra triplet of vacA genes with ≤50% protein-level identity to each other in the 5′ two-thirds of the gene needed for host factor interaction; (ii) divergent sets of outer membrane protein genes; (iii) several metabolic genes distinct from those of H. pylori; (iv) genes for an iron-cofactored urease related to those of Helicobacter species from terrestrial carnivores, in addition to genes for a nickel co-factored urease; and (v) members of the slr multigene family, some of which modulate host responses to infection and improve Helicobacter growth with mammalian cells.ConclusionsOur genome sequence data provide a glimpse into the novelty and great genetic diversity of marine helicobacters. These data should aid further analyses of microbial genome diversity and evolution and infection and disease mechanisms in vast and often fragile ocean ecosystems.

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Laboratory Maintenance of Helicobacter Species

Cited by 40 publications

References 20 publications

Comparison of media and growth conditions for culturing enterohepaticHelicobacterspecies

Comparison of media and growth conditions for culturing enterohepaticHelicobacterspecies

The Family Helicobacteraceae

Sequence Divergence and Conservation in Genomes of Helicobacter cetorum Strains from a Dolphin and a Whale

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