Maintenance of 2,3-DPG and ATP Levels in Blood Stored for 30 Days at a Constant pH Obtained by Variation of C0(2)

Abstract

A novel procedure for storing blood at a controlled pH consists in collecting blood in a pH 8.20, Tris-CPD solution and storing it in a special recipient including a gas permeable membrane under a CO(2) atmosphere. The recipient is placed in an atmosphere of variable CO(2)content, so that the initial alkalinity of the preservative is balanced by dissolved CO(2), the proportion of which is diminished when lactate production increases with storage. 2,3-DPG and ATP were studied at three different pH levels of approximately 7.25, 7.45, and 7.65 at 4°C. The best pH for the simultaneous maintenance of 2,3-DPG and ATP was 7.65. Under these conditions, 2,3-DPG is maintained at its initial level and ATP at 55% of its initial level at the 30th day. Lactate production is linear and hemolysis moderate.

Storage of red blood cells with improved maintenance of 2,3‐bisphosphoglycerate

“…Other attempts to neutralize the lactic acid formed during storage include amberlite ion‐exchanging beads included with the RBCs 23 and the use of a gas‐permeable bag with storage in an environment where the partial pressure of CO 2 can be adjusted 24 . Bensinger and associates, 25 using bicarbonate buffer, maintained pH during storage by including in the storage bag a pouch permeable to bicarbonate and containing sodium hydroxide, which neutralized carbonic acid 25 .…”

Studies in Red Blood Cell Preservation

“…Rapoport and Guest [37] first showed that DPG is rapidly decomposed in acidified blood while ATP is better maintained. Higher pH levels are associated with an increased rate of glycolysis [38, 391. De Mendonca et al [40] found that an extracorpuscular pH of 7.65 at a presumed intracorpuscular pH of 7.40 was best for maintaining DPG levels at initial levels for 30 days, but in their system ATP dropped to 55% of its starting level.…”

Studies in Red Blood Cell Preservation

“…Rapoport and Guest [37] first showed that DPG is rapidly decomposed in acidified blood while ATP is better maintained. Higher pH levels are associated with an increased rate of glycolysis [38,39], De Mendonca et al [40] found that an extracorpuscular pH of 7.65 at a presumed intracorpuscular pH of 7.40 was best for main taining DPG levels at initial levels for 30 days, but in their system ATP dropped to 55% of its starting level.…”