Maine Caucasian Population DNA Database Using Twelve Short Tandem Repeat Loci

Kupferschmid, Timothy D.; Calicchio, Theresa; Budowle, Bruce

doi:10.1520/jfs14471j

Cited by 20 publications

(6 citation statements)

References 12 publications

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“…This kit uses multiplex PCR amplification of nine short tandem repeat loci (D3S1358, VWA, FGA, D5S818, D13S317, D7S820, D8S1179, D21S11 and D18S51) plus the gender marker Amelogenin that allows quantification of the percentage of donor and recipient DNA in the sample studied. Because of the large number of loci that can be analysed, the power of discrimination is greater than 99% in various caucasian populations (Entrala et al , 1998; Kupferschmid et al , 1999).…”

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confidence: 99%

Allogeneic peripheral blood stem cell transplantation with reduced‐intensity conditioning: results of a prospective multicentre study

et al. 2001

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Summary. Reduced-intensity conditioning (RIC) regimens for allogeneic haematopoietic stem cell transplantation (SCT) have been shown to lead to engraftment of donor stem cells without the severe extra-haematological toxicities of traditional myeloablative transplants. Between December 1998 and December 2000, 76 patients underwent a RIC peripheral blood SCT in a prospective multicentre study. The median age was 53 years, and 57 patients were beyond the early phase of their disease. The conditioning regimens consisted of fludarabine (150 mg/ m 2 ) plus melphalan (140 mg/m 2 ) or busulphan (10 mg/kg). Graft-versus-host disease (GVHD) prophylaxis consisted of cyclosporin A plus short-course methotrexate. The preparative regimens were well tolerated. All patients experienced severe pancytopenia, but haematological recovery was prompt in all but two cases (early deaths). The 100-d probability of developing grade II±IV acute GVHD was 32% (10% grade III±IV), and the 1-year probability of developing chronic extensive GVHD was 43%. Early complete donor chimaerism was observed in 52/68 patients, and 16 evaluable patients were in complete chimaerism 1 year post transplant. With a median follow-up of 283 d (355 in 48 survivors), the 1-year probability of transplant-related mortality was 20%, and the 1-year overall and progression-free survivals were 60% and 55% respectively. In conclusion, RIC regimens lead to low early toxicity after allografting, with stable donor haematopoietic engraftment, with an apparent low risk of acute GVHD. Chronic GVHD, however, develops in a significant proportion of patients.

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confidence: 99%

Allogeneic peripheral blood stem cell transplantation with reduced‐intensity conditioning: results of a prospective multicentre study

et al. 2001

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“…In the last decade, DNA parentage tests have been widely applied to forensic cases Kupferschmid et al, 1999). Thirteen autosomal STRs of the combined DNA index system (CODIS), D5S818, FGA, D3S1358, TH01, D13S317, D16S539, D8S1179, D21S11, D7S820, CSF1PO, vWA, TPOX, and D18S51, are the basic loci of choice for forensic use.…”

Section: Discussionmentioning

confidence: 99%

Risk analysis of duo parentage testing with limited STR loci

Zhang¹,

Gao²,

Han³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. The aim of this study was to evaluate whether the Goldeneye 20A system (containing 19 short tandem repeats) can avert the shortage of duo parentage tests. Among routine cases typed by the Identifiler system, we identified 42 motherless cases, 2 fatherless cases, and 34 trio cases containing 1 locus mismatch and 4 motherless cases with 2 locus mismatches. One true trio case was rejected by fatherhood testing because of the omission of the mother's genotype and because the genotype of the putative father matched that of the child. All of the cases were retyped by the Goldeneye 20A system with the mother's or father's sample. In total, 39 motherless cases were verified by one mutation, 3 motherless cases were rejected for paternity, and 4 motherless cases with 2 locus mismatches were ruled out by fatherhood testing. After adding the father's genotype, 1 motherless case was confirmed by a single-locus mutation, whereas another case was rejected by motherhood testing. The mutation and exclusion rates detected with the Goldeneye 20A system accorded with the corresponding rates identified in the Identifiler system. The trio case also rejected fatherhood without the mother's genotype, and we found only 2 locus mismatches. Neither the Identifiler system nor the Goldeneye 20A system compensates for the absence of genetic information from the mother or father.

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“…Therefore we performed co-amplification of 12 sensitive and reliable STR loci with a combined probability of exclusion of > 0.99999999 (Hantschel et al 1999;Kupferschmid et al 1999;Neuhuber et al 1999). Staining with HE prior to laser microdissection obviously did not affect PCR amplification (Banaschak et al 2000).…”

Section: Discussionmentioning

confidence: 99%

Paternity testing after pregnancy termination using laser microdissection of chorionic villi

Bauer¹,

Thalheimer

Patzelt³

2002

International Journal of Legal Medicine

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We report an unusual case of paternity testing from residues of chorionic villi 5 weeks after pregnancy termination. The autopsy of a 32-year-old female homicide victim revealed the presence of intact chorionic villi at the former placenta implantation site. Fetal cells were selectively isolated by laser-induced microdissection of the remaining villi to avoid contamination with maternal DNA. Simultaneous amplification of 12 STR loci in 2 PCR reactions resulted in a combined probability of paternity of 99.94%. This case demonstrates that laser-assisted microdissection and multiplex STR typing provide tools for paternity testing performed on endometrial mucosa long after the product of conception was removed by therapeutic abortion.

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Maine Caucasian Population DNA Database Using Twelve Short Tandem Repeat Loci

Cited by 20 publications

References 12 publications

Allogeneic peripheral blood stem cell transplantation with reduced‐intensity conditioning: results of a prospective multicentre study

Allogeneic peripheral blood stem cell transplantation with reduced‐intensity conditioning: results of a prospective multicentre study

Risk analysis of duo parentage testing with limited STR loci

Paternity testing after pregnancy termination using laser microdissection of chorionic villi

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