Main Concepts of Chemical and Biological Sensing

Trojanowicz, Marek

doi:10.1007/978-0-387-73713-3_2

Cited by 5 publications

(6 citation statements)

References 130 publications

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“…[ 79–81 ] Besides, the choice of a protein as the template molecule allows demonstrating the robustness of the vapor‐phase imprinting procedure on macromolecules, which is intrinsically more challenging than that of small molecules. [ 5,82–84 ]…”

Section: Introductionmentioning

confidence: 99%

Vapor‐Phase Synthesis of Molecularly Imprinted Polymers on Nanostructured Materials at Room‐Temperature

Mazzotta

Giulio

Mariani

et al. 2023

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Molecularly imprinted polymers (MIPs) have recently emerged as robust and versatile artificial receptors. MIP synthesis is carried out in liquid phase and optimized on planar surfaces. Application of MIPs to nanostructured materials is challenging due to diffusion‐limited transport of monomers within the nanomaterial recesses, especially when the aspect ratio is >10. Here, the room temperature vapor‐phase synthesis of MIPs in nanostructured materials is reported. The vapor phase synthesis leverages a >1000‐fold increase in the diffusion coefficient of monomers in vapor phase, compared to liquid phase, to relax diffusion‐limited transport and enable the controlled synthesis of MIPs also in nanostructures with high aspect ratio. As proof‐of‐concept application, pyrrole is used as the functional monomer thanks to its large exploitation in MIP preparation; nanostructured porous silicon oxide (PSiO2) is chosen to assess the vapor‐phase deposition of PPy‐based MIP in nanostructures with aspect ratio >100; human hemoglobin (HHb) is selected as the target molecule for the preparation of a MIP‐based PSiO2 optical sensor. High sensitivity and selectivity, low detection limit, high stability and reusability are achieved in label‐free optical detection of HHb, also in human plasma and artificial serum. The proposed vapor‐phase synthesis of MIPs is immediately transferable to other nanomaterials, transducers, and proteins.

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Section: Introductionmentioning

confidence: 99%

Vapor‐Phase Synthesis of Molecularly Imprinted Polymers on Nanostructured Materials at Room‐Temperature

Mazzotta

Giulio

Mariani

et al. 2023

Small

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“…They are highly sensitive and selective, but the analyses are performed off-line and typically are time and labor intensive, thus limiting their applications. Therefore, inexpensive, highly sensitive chemical/biochemical sensors with ability of quick measurement are still in great demand [ 1 , 2 ].…”

Section: Introductionmentioning

confidence: 99%

Biotin-Streptavidin Binding Interactions of Dielectric Filled Silicon Bulk Acoustic Resonators for Smart Label-Free Biochemical Sensor Applications

Heidari

Yoon

Park

et al. 2014

Sensors

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Sensor performance of a dielectric filled silicon bulk acoustic resonator type label-free biosensor is verified with biotin-streptavidin binding interactions as a model system. The mass sensor is a micromachined silicon square plate with a dielectric filled capacitive excitation mechanism. The resonance frequency of the biotin modified resonator decreased 315 ppm when exposed to streptavidin solution for 15 min with a concentration of 10−7 M, corresponding to an added mass of 3.43 ng on the resonator surface. An additional control is added by exposing a bovine serum albumin (BSA)-covered device to streptavidin in the absence of the attached biotin. No resonance frequency shift was observed in the control experiment, which confirms the specificity of the detection. The sensor-to-sensor variability is also measured to be 4.3%. Consequently, the developed sensor can be used to observe in biotin-streptavidin interaction without the use of labelling or molecular tags. In addition, biosensor can be used in a variety of different immunoassay tests.

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“…Both electrochemical and electrical impedance spectroscopy (EIS) are widespread techniques that, over the last few years, have become increasingly popular in fields such as the food industry [1] or medical diagnosis [2] among others. For example, a proof of this success is that in the recent years, modern chemical analysis has been revolutionized by electrochemical biosensors [3] because of their accuracy, technical simplicity, high efficiency, portability and miniaturization. The fact that binding biomolecules to 3 Author to whom any correspondence should be addressed.…”

Section: Introductionmentioning

confidence: 99%

“…For example, a proof of this success is that in the recent years, modern chemical analysis has been revolutionized by electrochemical biosensors [3] because of their accuracy, technical simplicity, high efficiency, portability and miniaturization. The fact that binding biomolecules to 3 Author to whom any correspondence should be addressed. solid supports changes their physical properties such as the interface potential has been exploited using a wide variety of sensing systems, i.e.…”

Section: Introductionmentioning

confidence: 99%

Basics of broadband impedance spectroscopy measurements using periodic excitations

Sanchez¹,

Vandersteen²,

Bardia³

et al. 2012

Meas. Sci. Technol.

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Measuring the impedance frequency response of systems by means of frequency sweep electrical impedance spectroscopy (EIS) takes time. An alternative based on broadband signals enables the user to acquire simultaneous impedance response data collection. This is directly reflected in a short measuring time compared to the frequency sweep approach. As a result of this increase in the measuring speed, the accuracy of the impedance spectrum is compromised. The aim of this paper is to study how the choice of the broadband signal can contribute to mitigate this accuracy loss. A review of the major advantages and pitfalls of four different periodic broadband excitations suitable to be used in EIS applications is presented. Their influence on the instrumentation and impedance spectrum accuracy is analyzed. Additionally, the signal processing tools to objectively evaluate the quality of the impedance spectrum are described. In view of the experimental results reported, the impedance spectrum signal-to-noise ratio (SNRZ) obtained with multisine or discrete interval binary sequence signals is about 20–30 dB more accurate than maximum length binary sequence or chirp signals.

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Main Concepts of Chemical and Biological Sensing

Cited by 5 publications

References 130 publications

Vapor‐Phase Synthesis of Molecularly Imprinted Polymers on Nanostructured Materials at Room‐Temperature

Vapor‐Phase Synthesis of Molecularly Imprinted Polymers on Nanostructured Materials at Room‐Temperature

Biotin-Streptavidin Binding Interactions of Dielectric Filled Silicon Bulk Acoustic Resonators for Smart Label-Free Biochemical Sensor Applications

Basics of broadband impedance spectroscopy measurements using periodic excitations

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