Mahuannin B an adenylate cyclase inhibitor attenuates hyperhidrosis via suppressing β 2 -adrenoceptor/cAMP signaling pathway

Wang, Zengyong; Cui, Yu Jia; Ding, Guoyu; Zhou, Mei; Ma, Xuan; Hou, Yuanyuan; Jiang, Min; Liu, Dailin; Bai, Gang

doi:10.1016/j.phymed.2017.03.002

Cited by 11 publications

(7 citation statements)

References 25 publications

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“…Peak 1 was confirmed as ephedrine, since it had a m/z value of 166.11484, which gave a mass difference of −8.4 mmu compared to the theoretical value of ephedrine and retention order in reversed phase silica-based chromatography. Peak 2 was confirmed as pseudoephedrine by direct comparison of its spectroscopic and chromatographic characteristics with the data previously reported [14]. The X-ray diffraction (XRD) of GF is shown in Figure 2.…”

Section: Identification Of Ephedra Sinica Stapf Extract (Ehe) and Gypsumsupporting

confidence: 71%

Ephedra sinica Stapf and Gypsum Attenuates Heat-Induced Hypothalamic Inflammation in Mice

Kim

Lee

Huh

et al. 2019

Toxins

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Ephedra sinica Stapf (EH) exert toxic effects, such as excitability, cardiac arrhythmia, and others. On the contrary, in traditional herbal medicine, EH and gypsum (GF) are used most often to treat symptoms caused by external stressors. The hypothalamus plays a crucial role in thermal homeostasis. Inflammatory response in the hypothalamus by thermal stressors may affect thermal and energy homeostasis. This study investigates the effect of EH and GF against heat-induced mouse model. Mice were divided into four groups: saline, saline plus heat, EH plus heat, and GF plus heat treated groups. Heat stress was fixed at 43 • C for 15 min once daily for 3 days. Weight and ear and rectal temperature measurements were made after terminating heat stress. Hypothalamus tissue was collected to evaluate the HSP70, nuclear factor kappa-B (NF-kB), and interleukin (IL)-1β protein expression levels. EH and GF treatment suppressed the increased body temperature. EH significantly ameliorated heat-induced body weight loss, compared to gypsum. Regulatory effects of EH and GF for body temperature and weight against heat stress were mediated by IL-1β reduction. EH showed significant HSP70 and NF-kB inhibition against heat stress. EH and GF contribute to the inhibition of heat-induced proinflammatory factors and the promotion of hypothalamic homeostasis. Key Contribution: Treatment with Ephedra sinica Stapf and gypsum inhibits heat-induced proinflammatory factors and ameliorate hypothalamic homeostasis. Author Contributions: Conceptualization: W.K. and M.S.O.; investigation: W.L., E.H., and E.C.; formal analysis: W.K., W.L., and Y.P.J.; resources: Y.-K.K. and T.-H.L.; writing-original draft preparation: W.K. and M.S.O.; writing-review and editing: Y.P.J., Y.-K.K., and T.-H.L.; visualization: W.K.; supervision: W.K. and M.S.O.; funding acquisition: M.S.O. All authors have read and agreed to the published version of the manuscript.

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Section: Identification Of Ephedra Sinica Stapf Extract (Ehe) and Gypsumsupporting

confidence: 71%

Ephedra sinica Stapf and Gypsum Attenuates Heat-Induced Hypothalamic Inflammation in Mice

Kim

Lee

Huh

et al. 2019

Toxins

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“…Phytochemical profile differences among several populations were identified using a metabolomic approach by the use of UPLC-Q/TOF-MS, PCA analysis and molecular docking. From this plant, the phenolics mahuannins B ( 93 ), D ( 95 ), E ( 96 ) and F ( 97 ), as well as, ephedrannin A ( 98 ) and herbacetin 8-methyl ether 3- O -glucoside ( 69 ) were isolated, displaying effects as anti-hydrotics [ 90 ]. The phytochemical study of aqueous and methanolic extracts of E. foeminea Forssk.…”

Section: Chemistrymentioning

confidence: 99%

A Review of the Ephedra genus: Distribution, Ecology, Ethnobotany, Phytochemistry and Pharmacological Properties

et al. 2020

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Ephedra is one of the largest genera of the Ephedraceae family, which is distributed in arid and semiarid regions of the world. In the traditional medicine from several countries some species from the genus are commonly used to treat asthma, cold, flu, chills, fever, headache, nasal congestion, and cough. The chemical constituents of Ephedra species have been of research interest for decades due to their contents of ephedrine-type alkaloids and its pharmacological properties. Other chemical constituents such as phenolic and amino acid derivatives also have resulted attractive and have provided evidence-based supporting of the ethnomedical uses of the Ephedra species. In recent years, research has been expanded to explore the endophytic fungal diversity associated to Ephedra species, as well as, the chemical constituents derived from these fungi and their pharmacological bioprospecting. Two additional aspects that illustrate the chemical diversity of Ephedra genus are the chemotaxonomy approaches and the use of ephedrine-type alkaloids as building blocks in organic synthesis. American Ephedra species, especially those that exist in Mexico, are considered to lack ephedrine type alkaloids. In this sense, the phytochemical study of Mexican Ephedra species is a promising area of research to corroborate their ephedrine-type alkaloids content and, in turn, discover new chemical compounds with potential biological activity. Therefore, the present review represents a key compilation of all the relevant information for the Ephedra genus, in particular the American species, the species distribution, their ecological interactions, its ethnobotany, its phytochemistry and their pharmacological activities and toxicities, in order to promote clear directions for future research.

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“…The NMR data mentioned above, together with the mass spectrometric information, implied that the structure of this proanthocyanidin, designated as ephedrannin B, was proposed to be 5,7,4′-trihydroxyflavan-[4(α) → 8,2(α) → O → 7]-kaempferol, which was previously reported in the roots of Ephedra sinica [ 40 ] ( Supplementary Material; Figures S19–S21 ). Ephedrannin B was previously isolated from the roots of E. sinica , and exerts anti-inflammatory action [ 41 ], anti-hydrotic [ 42 ] and antimicrobial activities, and depigmentation effects on the skin [ 42 ].…”

Section: Resultsmentioning

confidence: 99%

Bio-Guided Assay of Ephedra foeminea Forssk Extracts and Anticancer Activities: In Vivo, In Vitro, and In Silico Evaluations

Nazeam,

Boshra,

Mohammed

et al. 2023

Molecules

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Ephedra is one of the oldest known medicinal plants and the largest genera of the Ephedraceae family. In vivo antitumor evaluation of Ephedra foeminea revealed that ethyl acetate (EtOAc) was the most bioactive fraction. Bio-guided fractionation of EtOAc fraction afforded nine compounds isolated for the first time from the plant species. Macrocyclic spermine alkaloids (1,9), proanthocyanidins (2,4,5), quinoline alkaloids (7,8), phenolic (3), and nucleoside (6) were identified and elucidated by spectroscopic analyses including 1D and 2D NMR, ESI-MS-MS spectrometry. The tested compounds exhibited moderate anticancer activity, except for the kynurenic acid derivative (6-mKYNA) which showed significant cytotoxicity and remarkable inhibition of CA-19.9 and CA-125 tumor biomarkers. In-silico study was conducted to determine the anti-proliferative mechanism of 6-mKYNA by using the CK2 enzyme active site. Moreover, the ADME computational study suggested that 6-mKYNA is an effective candidate with a promising pharmacokinetic profile and therapeutic potential against various types of cancer.

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Mahuannin B an adenylate cyclase inhibitor attenuates hyperhidrosis via suppressing β 2 -adrenoceptor/cAMP signaling pathway

Cited by 11 publications

References 25 publications

Ephedra sinica Stapf and Gypsum Attenuates Heat-Induced Hypothalamic Inflammation in Mice

Ephedra sinica Stapf and Gypsum Attenuates Heat-Induced Hypothalamic Inflammation in Mice

A Review of the Ephedra genus: Distribution, Ecology, Ethnobotany, Phytochemistry and Pharmacological Properties

Bio-Guided Assay of Ephedra foeminea Forssk Extracts and Anticancer Activities: In Vivo, In Vitro, and In Silico Evaluations

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