Magnolol protects channel catfish from Aeromonas hydrophila infection via inhibiting the expression of aerolysin

Dong, Jing; Ding, Hao; Liu, Yongtao; Yang, Qiuhong; Xu, Ning; Yang, Yibin; Ai, Xiaohui

doi:10.1016/j.vetmic.2017.10.005

Cited by 31 publications

(33 citation statements)

References 31 publications

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“…Moreover, the hlyA and aerA gene were identified in all the virulent A. hydrophila strains ( Heuzenroeder et al, 1999 ). Consequently, AerA and HlyA were recognized as potent targets in identifying novel drugs ( Dong et al, 2017a ). Several works have determined the structure of AerA and mechanism of forming pore ( Parker et al, 1994 ; Degiacomi et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

“…There have been several successful attempts identifying small molecules inhibiting the activity or expression of AerA and antibodies. In our previous study, we found that magnolol isolated from traditional Chinese medicine could significantly reduce the mortality of channel catfish infected with A. hydrophila via inhibiting the transcription of aerA ( Dong et al, 2017a ). In this paper, another natural compound named morin was identified that could directly inhibit the activity of AerA.…”

Section: Discussionmentioning

confidence: 99%

“…Hemolytic activities were performed using purified AerA and supernatants of A. hydrophila co-cultured with different concentrations of morin ranging from 1 to 8 μg/mL. Hemolytic assays were performed according to previous reports ( Dong et al, 2013 ; Dong et al, 2017a ). For hemolytic assay of co-cultured supernatant, 100 μL of trypsin treated supernatants were combined with 25 μL defibrinated sheep red blood cells (5 × 10 6 cells/ml) in hemolytic buffer (20 mM Tris, 150 mM NaCl, pH 7.2).…”

Section: Methodsmentioning

confidence: 99%

“…Although A. hydrophila is not a typical pathogen for human, it has been reported to be responsible for a range of infections including septicemia, wound infections, burn-associated sepsis, and respiratory tract infections ( Santos et al, 1999 ; Casabianca et al, 2015 ). Antibiotics are the main approach in the treatment of infections caused by bacterium ( Dong et al, 2017a ). However, the abuse of antibiotics in aquaculture leads to emergence of antibiotic resistance and environmental pollution ( Zhang et al, 2015 ; Stratev and Odeyemi, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

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Morin Protects Channel Catfish From Aeromonas hydrophila Infection by Blocking Aerolysin Activity

Dong

Liu

et al. 2018

Front. Microbiol.

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Aeromonas hydrophila (A. hydrophila) is an opportunistic bacterial pathogen widely distributed in the environments, particular aquatic environment. The pathogen can cause a range of infections in both human and animals including fishes. However, the application of antibiotics in treatment of A. hydrophila infections leads to the emergence of resistant strains. Consequently, new approaches need to be developed in fighting this pathogen. Aerolysin, the chief virulence factor produced by pathogenic A. hydrophila strains has been employed as target identifying new drugs. In our present study, we found that morin, a flavonoid without anti-bacterial activity isolated from traditional Chinese medicine, could directly inhibit the hemolytic activity of aerolysin. To determine the binding sites and the action of mechanism of morin against AerA, several assays were performed. Ser36, Pro347, and Arg356 were identified as the main binding sites affecting the conformation of AerA and resulted in block of the heptameric formation. Moreover, morin could protect Vero cells from cell injury mediated by aerolysin. In vivo study showed that morin could provide a protection to channel catfish against A. hydrophila infection. These results demonstrated that morin could be developed as a promising candidate for the treatment of A. hydrophila infections by decreasing the pathogenesis of A. hydrophila.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Morin Protects Channel Catfish From Aeromonas hydrophila Infection by Blocking Aerolysin Activity

Dong

Liu

et al. 2018

Front. Microbiol.

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“…Among these problems, infections caused by pathogenic Aeromonas hydrophila (A. hydrophila) have been regarded as a major concern to freshwater fish aquaculture, often leading to severe losses globally [1]. In addition, A. hydrophila is an opportunistic pathogen to human and terrestrial animals that can cause a range of infections, such as diarrhea, septicemia, and meningitis [3]. Infections caused by A. hydrophila can transmit from diseased fish to humans by intake of contaminated water and undercooked fish [4].…”

Section: Introductionmentioning

confidence: 99%

Thymol Protects Channel Catfish from Aeromonas hydrophila Infection by Inhibiting Aerolysin Expression and Biofilm Formation

et al. 2020

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Aeromonas hydrophila is an opportunistic pathogen responsible for a number of diseases in freshwater farming. Moreover, the bacterium has been identified as a zoonotic pathogen that threatens human health. Antibiotics are widely used for treatments of infectious diseases in aquaculture. However, the abuse of antibiotics has led to the emergence of antimicrobial resistant strains. Thus, novel strategies are required against resistant A. hydrophila strains. The quorum sensing (QS) system, involved in virulence factor production and biofilm formation, is a promising target in identifying novel drugs against A. hydrophila infections. In this study, we found that thymol, at sub-inhibitory concentrations, could significantly reduce the production of aerolysin and biofilm formation by inhibiting the transcription of genes aerA, ahyI, and ahyR. These results indicate that thymol inhibits the quorum sensing system. The protective effects of thymol against A. hydrophila mediated cell injury were determined by live/dead assay and lactate dehydrogenase (LDH) release assay. Moreover, the in vivo study showed that thymol could significantly decrease the mortality of channel catfish infected with A. hydrophila. Taken together, these findings demonstrate that thymol could be chosen as a phytotherapeutic candidate for inhibiting quorum sensing system-mediated aerolysin production and biofilm formation in A. hydrophila.

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Antibacterial activity of a short de novo designed peptide against fish bacterial pathogens

Bhat,

Khangembam,

Pant

et al. 2024

Amino Acids

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In the face of increasing antimicrobial resistance in aquaculture, researchers are exploring novel substitutes to customary antibiotics. One potential solution is the use of antimicrobial peptides (AMPs). We aimed to design and evaluate a novel, short, and compositionally simple AMP with potent activity against various bacterial pathogens in aquaculture. The resulting peptide, KK12YW, has an amphipathic nature and net charge of + 7. Molecular docking experiments disclosed that KK12YW has a strong affinity for aerolysin, a virulence protein produced by the bacterial pathogen Aeromonas sobria. KK12YW was synthesized using Fmoc chemistry and tested against a range of bacterial pathogens, including A. sobria, A. salmonicida, A. hydrophila, Edwardsiella tarda, Vibrio parahaemolyticus, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus epidermidis, and methicillin-resistant S. aureus. The AMP showed promising antibacterial activity, with MIC and MBC values ranging from 0.89 to 917.1 µgmL−1 and 3.67 to 1100.52 µgmL−1, respectively. In addition, KK12YW exhibited resistance to high temperatures and remained effective even in the presence of serum and salt, indicating its stability. The peptide also demonstrated minimal hemolysis toward fish RBCs, even at higher concentrations. Taken together, these findings indicate that KK12YW could be a highly promising and viable substitute for conventional antibiotics to combat microbial infections in aquaculture.

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Magnolol protects channel catfish from Aeromonas hydrophila infection via inhibiting the expression of aerolysin

Cited by 31 publications

References 31 publications

Morin Protects Channel Catfish From Aeromonas hydrophila Infection by Blocking Aerolysin Activity

Morin Protects Channel Catfish From Aeromonas hydrophila Infection by Blocking Aerolysin Activity

Thymol Protects Channel Catfish from Aeromonas hydrophila Infection by Inhibiting Aerolysin Expression and Biofilm Formation

Antibacterial activity of a short de novo designed peptide against fish bacterial pathogens

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