Magnetosome-anti-Salmonella antibody complex based biosensor for the detection of Salmonella typhimurium

Sannigrahi, Sumana; Arumugasamy, Shiva Kumar; Mathiyarasu, J.; Suthindhiran, K.

doi:10.1016/j.msec.2020.111071

Cited by 29 publications

(21 citation statements)

References 85 publications

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“…Immunology based approaches such as enzyme-linked immunosorbent assay (ELISA), thermometric immunoassay, lateral flow assay, and immunomagnetic separation assay were extensively employed for the recognition of deadly microorganisms in complex food and polluted water samples. [148] Although diagnostic approaches based on immunology are not more precise than nucleic acid-based tests, they are more reliable, rapid and have the ability to detect pathogens. [149] Furthermore, biotoxins which may not be expressed in the genome of the organism could also be detected by the immunology-based approach.…”

Section: Immunology-based Methodsmentioning

confidence: 99%

“…For the detection of antigen epitopes of target pathogens, the walls of the microtiter plate wells are coated with primary antibody. [148] When the microtiter plate wells are exposed to the contaminated food samples, the immobilized primary antibody specifically binds to its target antigen and the residual unbound antigens are detached. Consequently, the secondary antibody is tagged with an enzyme and the complex is added to the microtiter plate wells.…”

Section: Enzyme-linked Immunosorbent Assaymentioning

confidence: 99%

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Principles and Recent Advances in Biosensors for Pathogens Detection

et al. 2021

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Foodborne and waterborne diseases caused by pathogens pose a serious threat to human life, and the detection of such pathogens in food, water, and beverages has gained paramount importance in view of the increasing number of pathogenic diseases in recent times. Standard and traditional analytical techniques employed for the recognition of deadly pathogens, including polymerase chain reaction‐based techniques, immunology‐based assays, culture and colony counting techniques, require several hours or perhaps even a few days for the results. All these techniques, despite being quite sensitive, are time‐intensive. Therefore, several researchers are focusing on the development of rapid pathogen detection techniques. Although the most advanced biosensing technologies exhibit potential approaches, significant research and testing is an urgent need to design innovative biosensors. Novel bioanalytical approaches for the recognition and quantification of target pathogens are being designed and developed to enhance the characteristics of biosensors, including rapid response, selectivity, and sensitivity. In this context, we not only provide an overview of the types of biorecognition elements employed for the detection of pathogens, but also discuss in detail the traditional approaches, biomolecular techniques, the latest advances in the detection and quantification of foodborne and waterborne pathogens, with particular emphasis on biosensors.

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Section: Immunology-based Methodsmentioning

confidence: 99%

Section: Enzyme-linked Immunosorbent Assaymentioning

confidence: 99%

Principles and Recent Advances in Biosensors for Pathogens Detection

et al. 2021

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“…A study by Sannigrahi et al used magnetosome (biogenic nanoparticles synthesised in a magnetotactic bacteria Magnetospirillum sp. through biomineralisation) functionalised with anti- Salmonella antibody to detect lipopolysaccharide (somatic “O” antigen) of S. Typhimurium in food and water samples [ 194 ]. Electrochemical impedance spectroscopy (EIS) confirmed the detection of lipopolysaccharide at 0.001–0.1 μg/mL and the LOD of bacteria at 1 × 10 1 CFU/mL in water and milk samples.…”

Section: Biosensors Developed For Salmonella Detectionmentioning

confidence: 99%

Advancement in Salmonella Detection Methods: From Conventional to Electrochemical-Based Sensing Detection

et al. 2021

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Large-scale food-borne outbreaks caused by Salmonella are rarely seen nowadays, thanks to the advanced nature of the medical system. However, small, localised outbreaks in certain regions still exist and could possess a huge threat to the public health if eradication measure is not initiated. This review discusses the progress of Salmonella detection approaches covering their basic principles, characteristics, applications, and performances. Conventional Salmonella detection is usually performed using a culture-based method, which is time-consuming, labour intensive, and unsuitable for on-site testing and high-throughput analysis. To date, there are many detection methods with a unique detection system available for Salmonella detection utilising immunological-based techniques, molecular-based techniques, mass spectrometry, spectroscopy, optical phenotyping, and biosensor methods. The electrochemical biosensor has growing interest in Salmonella detection mainly due to its excellent sensitivity, rapidity, and portability. The use of a highly specific bioreceptor, such as aptamers, and the application of nanomaterials are contributing factors to these excellent characteristics. Furthermore, insight on the types of biorecognition elements, the principles of electrochemical transduction elements, and the miniaturisation potential of electrochemical biosensors are discussed.

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“…Salmonellosis represents a serious occupational and public health hazard. Characteristic lipopolysaccharide, which is composed of lipid-A and a major O-antigen with side chains of repeating units of sugar residues, is a dominant cause for Salmonellosis (Sannigrahi et al, 2020). On the basis of the O antigen, Salmonella has been divided into 46 serogroups that markedly differ in their virulence (Davies et al, 2013); the isolates of D, B, C1, C2, and E serogroups take up a great majority of foodborne outbreaks (Graziani et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Identification of Novel Sensitive and Reliable Serovar-Specific Targets for PCR Detection of Salmonella Serovars Hadar and Albany by Pan-Genome Analysis

Shang

Chen

et al. 2021

Front. Microbiol.

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The accurate and rapid classification of Salmonella serovars is an essential focus for the identification of isolates involved in disease in humans and animals. The purpose of current research was to identify novel sensitive and reliable serovar-specific targets and to develop PCR method for Salmonella C2 serogroups (O:8 epitopes) in food samples to facilitate timely treatment. A total of 575 genomic sequences of 16 target serovars belonging to serogroup C2 and 150 genomic sequences of non-target serovars were analysed by pan-genome analysis. As a result, four and three specific genes were found for serovars Albany and Hadar, respectively. Primer sets for PCR targeting these serovar-specific genes were designed and evaluated based on their specificity; the results showed high specificity (100%). The sensitivity of the specific PCR was 2.8 × 101–103 CFU/mL and 2.3 × 103–104 CFU/mL for serovars Albany and Hadar, respectively, and the detection limits were 1.04 × 103–104 CFU/g and 1.16 × 104–105 CFU/g in artificially contaminated raw pork samples. Furthermore, the potential functions of these serovar-specific genes were analysed; all of the genes were functionally unknown, except for one specific serovar Albany gene known to be a encoded secreted protein and one specific gene for serovars Hadar and Albany that is a encoded membrane protein. Thus, these findings demonstrate that pan-genome analysis is a precious method for mining new high-quality serovar-targets for PCR assays or other molecular methods that are highly sensitive and can be used for rapid detection of Salmonella serovars.

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Magnetosome-anti-Salmonella antibody complex based biosensor for the detection of Salmonella typhimurium

Cited by 29 publications

References 85 publications

Principles and Recent Advances in Biosensors for Pathogens Detection

Principles and Recent Advances in Biosensors for Pathogens Detection

Advancement in Salmonella Detection Methods: From Conventional to Electrochemical-Based Sensing Detection

Identification of Novel Sensitive and Reliable Serovar-Specific Targets for PCR Detection of Salmonella Serovars Hadar and Albany by Pan-Genome Analysis

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