Magnetoresistive immunosensor for the detection of Escherichia coli O157:H7 including a microfluidic network

Mujika, Maite; Arana, Sergio; Castaño, E.; Tijero, M.; Vilares, R.; Ruano-Lopez, Jesus M.; García‐Cruz, Álvaro; Sainz, L.; Berganza, J.

doi:10.1016/j.bios.2008.07.024

Cited by 120 publications

(71 citation statements)

References 17 publications

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“…[103][104][105][106][107][108][109][110][111][112][113] Among these methods, optical and electrochemical methods are the most frequently utilized due to their selectivity and sensitivity. Other than the above major methods, approaches such as nuclear magnetic resonance (NMR) spectroscopy, [114][115][116][117][118] magnetoresistive, 119 and acoustical 120,121 are also coupled to microfluidics for sensing application.…”

Section: Detection Methodsmentioning

confidence: 99%

Microfluidic sensing: state of the art fabrication and detection techniques

2011

J. Biomed. Opt.

175

141

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Section: Detection Methodsmentioning

confidence: 99%

Microfluidic sensing: state of the art fabrication and detection techniques

2011

J. Biomed. Opt.

175

141

View full text Add to dashboard Cite

“…Before introducing into microchip, bacterial cells were firstly mixed with the FITC-labeled antibodies, which supported an easily way to detect by charge coupled devices (CCD) in the microscope. Separation is achieved by bacteria-FITC-labeled antibodies complex captured by bead-beds in the chamber of microchannel [21], and detection is obtained by observing the fluorescent phenomena from the bead-beds.…”

Section: Introductionmentioning

confidence: 99%

Immunomagnetic separation and rapid detection of bacteria using bioluminescence and microfluidics

Qiu

Zhang

Chen

et al. 2009

Talanta

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a b s t r a c tThis paper describes an immunomagnetic separation of target bacterial cells from others by using magnetic bead. The surface of bead was coated with antibodies which can capture specific organism. The binding efficiency of immunomagnetic bead (IMB) capturing target bacterial cells was higher than 98% when the concentrations of target and interferent bacterial cells were at the same level. The concentration of bacteria was determined indirectly by detecting adenosine 5 -triphosphate (ATP) employing bioluminescence (BL) reaction of firefly luciferin-ATP. Benzalkonium chloride (BAC) was used as an ATP extractant from living bacterial cells. We found that BAC could enhance the light emission when the concentration of BAC was less than 5.3 × 10 −2 % (w/v) and the BL intensity reached its maximum at the concentration of BAC was 2.7 × 10 −2 %, which was 10-fold stronger than that without BAC. Based on the principle of the IMB, a microfluidic chip combined with immunofluorescence assay for separating and detecting bacteria simultaneously was also developed. The IMBs were magnetically fixed in the beadbeds of chip channels with a 3-mm diameter of NdFeB permanent magnet. The target bacterial cells can be captured magnetically and observed by a fluorescent microscope.

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“…For example, an impedance biosensor system based on a combination of magnetic nanobeads coated with avian influenza virus subtype-specific antibody for capture (separation and concentration) of a target virus, followed by impedance measurement of the nanobeads-virus complexes, has been developed. 84 A magnetoresistive immunosensor 80 for detection of E. coli, which can detect small variations in the magnetic field caused by the conjugation of magnetic beads to previously immobilized antigens on the surface, has been reported.…”

Section: Sample Preparationmentioning

confidence: 99%

“…Micro-and nanobeads have been widely used for various applications such as nucleic acid enrichment [78][79] and whole-cell enrichment. 54,[80][81] A magnetic nanobead amplification-based QCM immunosensor, 82 with magnetic nanobeads coated with anti-H5 antibodies for amplification of the binding reaction between antibody and the H5N1 virus, has been developed. Magnetic micro-and nanobeads provide a means for better particle manipulation when compared to the nonmagnetic micro-and nanobeads.…”

Section: Sample Preparationmentioning

confidence: 99%

Development and Applications of Portable Biosensors

2015

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The significance of microfluidics-based and microelectromechanical systems-based biosensors has been widely acknowledged, and many reviews have explored their potential applications in clinical diagnostics, personalized medicine, global health, drug discovery, food safety, and forensics. Because health care costs are increasing, there is an increasing need to remotely monitor the health condition of patients by point-of-care-testing. The demand for biosensors for detection of biological warfare agents has increased, and research is focused on ways of producing small portable devices that would allow fast, accurate, and on-site detection. In the past decade, the demand for rapid and accurate on-site detection of plant disease diagnosis has increased due to emerging pathogens with resistance to pesticides, increased human mobility, and regulations limiting the application of toxic chemicals to prevent spread of diseases. The portability of biosensors for onsite diagnosis is limited due to various issues, including sample preparation techniques, fluid-handling techniques, the limited lifetime of biological reagents, device packaging, integrating electronics for data collection/analysis, and the requirement of external accessories and power. Many microfluidic, electronic, and biological design strategies, such as handling liquids in biosensors without pumps/valves, the application of droplet-based microfluidics, paper-based microfluidic devices, and wireless networking capabilities for data transmission, are being explored.

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Magnetoresistive immunosensor for the detection of Escherichia coli O157:H7 including a microfluidic network

Cited by 120 publications

References 17 publications

Microfluidic sensing: state of the art fabrication and detection techniques

Microfluidic sensing: state of the art fabrication and detection techniques

Immunomagnetic separation and rapid detection of bacteria using bioluminescence and microfluidics

Development and Applications of Portable Biosensors

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