The magnetoresistance ͑MR͒ behavior of epitaxial magnetite Fe 3 O 4 grown on low-vicinal ͑small miscut͒ and high-vicinal ͑large miscut͒ MgO substrates is compared. Magnetization measurements on Fe 3 O 4 films on high-vicinal substrates showed reduced magnetic moment as compared with the films grown on low-vicinal MgO, which correlates well with the expected reduction in magnetic moment due to step edge induced additional antiphase boundaries ͑APBs͒ with out-of-plane shift vectors. The MR is significantly higher ͑12.3% at 2 T͒ for a 45 nm Fe 3 O 4 film on high-vicinal substrate than that observed ͑7.2% at 2 T͒ for a film on low-vicinal substrate. A strong anisotropy in the MR is observed in correlation with the direction of atomic step edges. In addition to the increase in MR, the field dependency of the MR is also modified. The observed modification in the magnetotransport behavior of epitaxial Fe 3 O 4 films is attributed to an enhanced spin scattering arising due to the presence of atomic height steps that lead to the formation of a greater density of antiferromagnetically coupled APBs.