Magnetoliposomes Containing Multicore Nanoparticles and a New Antitumor Thienopyridine Compound with Potential Application in Chemo/Thermotherapy

Abstract

Multicore magnetic nanoparticles of manganese ferrite were prepared using carboxymethyl dextran as an agglutinating compound or by an innovative method using melamine as a cross-coupling agent. The nanoparticles prepared using melamine exhibited a flower-shape structure, a saturation magnetization of 6.16 emu/g and good capabilities for magnetic hyperthermia, with a specific absorption rate (SAR) of 0.14 W/g. Magnetoliposome-like structures containing the multicore nanoparticles were prepared, and their bilayer structure was confirmed by FRET (Förster Resonance Energy Transfer) assays. The nanosystems exhibited sizes in the range of 250–400 nm and a low polydispersity index. A new antitumor thienopyridine derivative, 7-[4-(pyridin-2-yl)-1H-1,2,3-triazol-1-yl]thieno[3,2-b]pyridine, active against HeLa (cervical carcinoma), MCF-7 (breast adenocarcinoma), NCI-H460 (non-small-cell lung carcinoma) and HepG2 (hepatocellular carcinoma) cell lines, was loaded in these nanocarriers, obtaining a high encapsulation efficiency of 98 ± 2.6%. The results indicate that the new magnetoliposomes can be suitable for dual cancer therapy (combined magnetic hyperthermia and chemotherapy).

Plasmonic/magnetic liposomes based on nanoparticles with multicore-shell architecture for chemo/thermotherapy

“…For comparison purposes, the mass concentration of the nanoparticles and clusters was certified to be the same. As expected, a wide absorption was observed for the MnFe2O4 nanoparticles, with a band at around 375 nm, in accordance with the previous observed for this type of NPs [11]. The higher absorption of the cluster nanostructures prepared with oxamide and melamine is consistent with a size increase, indicating the formation of large aggregates.…”

“…A similar behavior of magnetization decrease was previously observed for flower-shaped MnFe2O4 nanostructures, using melamine as cross-linking agent. In that work, the poor saturation magnetization of 6.16 emu/g was attributed to a weak internal ordering of the aggregates resulting in coupling of the spins of the cores within the multicore structures and dipole-dipole interactions of the clusters [11]. Nonetheless, the MnFe2O4 clusters prepared here with melamine show a higher saturation magnetization of 18.45 emu/g (table 1).…”

“…Spherical MnFe2O4 magnetic nanoparticles were prepared using the citrate stabilization method [11]. For that, 1 mmol of trisodium citrate dehydrate (Na3C6H5O7, 294.10 g/mol) and 0.47 mol of sodium hydroxide (NaOH, 18.9 M) were added to 19 mL of ultrapure water at 100 °C.…”

Upcycling of Polybenzoxazine to Magnetic Metal Nanoparticle-Doped Laser-Induced Graphene for Electromagnetic Interference Shielding

“…The SAR value of the LIG/Fe 3 O 4 -4 is calculated to be around 1.15 W/g. This value compares well or it is superior to the ones reported for manganese ferrite (0.14 W/g), polymer/Fe 3 O 4 composites (0.3–1.1 W/g), or graphene/Fe 3 O 4 hybrids (1.8–6.5 W/g) . Similarly, the LIG/FeCo sample, which has stronger magnetic properties, also displays significant magnetic-to-thermal conversion (Figure S18).…”

“…This also implies the uniform distribution of magnetic nanoparticles in LIG. The specific absorption rate (SAR) is used to evaluate the magneto-thermal conversion efficiency, which is defined as the amount of heat (per time and mass unit) absorbed by the sample under an AMF as expressed below where C p is the sample heat capacity, M / m is the mass ratio of the sample to the magnetic nanoparticles, and Δ T /Δ t is the initial slope of the temperature ( T )–time ( t ) curve in magneto-thermal conversion. The value of C p measured by DSC is 0.995 J g –1 K –1 (Figure S17).…”