Magnetite-doped polydimethylsiloxane (PDMS) for phosphopeptide enrichment

Sandison, Mairi E.; Jensen, Karina Tveen; Gesellchen, Frank; Cooper, Jonathan M.; Pitt, Andrew R.

doi:10.1039/c4an00750f

Cited by 8 publications

(5 citation statements)

References 44 publications

(49 reference statements)

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“…In summary, the on‐chip magnetic SPE‐MALDI‐TOF MS system with In 2 O 3 ‐coated Fe 3 O 4 MNPs as the adsorbent led to high selectivity, good reusability, and low detection limit when used for the detection of phosphopeptides. In addition, a comparison of the present system with some reported methods employing various magnetic metal oxides revealed that the present approach provided comparable selectivity, sensitivity, rapidness, and reagent dosage [, ].…”

Section: Resultsmentioning

confidence: 94%

“…Miniaturized total analysis system integrates many sample‐handling steps into a microchip with micrometer scale and has the advantages of reducing reagent wastage, shortening analysis time, and controlling liquid flow, which makes it receive universal attention in many fields . At present, extraction materials for SPE in microchips consist of membranes, polymer monoliths, and magnetic nanoparticles (MNPs) .…”

Section: Introductionmentioning

confidence: 99%

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Highly selective enrichment of phosphopeptides by on‐chip indium oxide functionalized magnetic nanoparticles coupled with MALDI‐TOF MS

Jiang

Song

et al. 2017

Proteomics

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Selective and efficient preconcentration is indispensable for low concentration of phosphopeptides in phosphorylated protein-related samples prior to MS-based analysis. Herein, an on-chip system coupled magnetic SPE with MALDI-TOF MS was designed. A metal oxide affinity chromatography material, indium oxide, was coated on the surface of Fe O magnetic nanoparticles to prepare the adsorbent, spatially confined with an applied magnetic field. The adsorbent exhibited high selectivity for phosphopeptides in tryptic digests of the mixture of β-casein and BSA (1:1000) and the mixture of β-casein, BSA, and ovalbumin (1:100:100). Thanking to the enrichment ability and specificity for phosphopeptides with the adsorbent, the on-chip magnetic SPE-MALDI-TOF MS approach showed high sensitivity with a low detection limit of 4 fmol. In addition, the developed approach was used to analyze phosphopetides in non-fat milk digests and human serum successfully.

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Section: Resultsmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Highly selective enrichment of phosphopeptides by on‐chip indium oxide functionalized magnetic nanoparticles coupled with MALDI‐TOF MS

Jiang

Song

et al. 2017

Proteomics

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“…Test materials were then etched, using a previously reported etchant solution for PDMS [35], to remove surface elastomer and expose the underlying nanoparticles, whilst control samples remained unetched. This method enables straightforward production of functional materials in a manner that can readily be customised (for example altering dopant materials and concentrations) and can be combined with other fabrication techniques, such as replica moulding [30] and extrusion, to produce medical devices.…”

Section: Resultsmentioning

confidence: 99%

“…However, when bulk doping polymers, the nanoparticles become embedded within the polymer matrix. Etching back the surface matrix can expose the underlying nanoparticles [30], simultaneously producing a roughened surface with high surface area (favourable to photocatalysis). This would allow for closer contact between the photoactive TiO 2 and attached bacteria, potentially increasing cytotoxic ROS exposure.…”

Section: Introductionmentioning

confidence: 99%

Metal oxide-doped elastomeric materials for amplifying visible light-based antimicrobial activity

McShea¹,

Kambo²,

Maclean³

et al. 2022

Mater. Res. Express

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Healthcare-associated infection through transmission of pathogenic bacteria poses a huge threat to public health. One of the main transmission routes is via contaminated surfaces, including those of medical devices, and therefore significant efforts are being invested in developing new surface decontamination strategies. This includes visible light-based approaches, which offer improved compatibility with mammalian cells but lower germicidal efficacy with respect to UV-light. This study investigates the potential to enhance the antimicrobial efficacy of 405 nm light for surface decontamination through use of a photocatalytic TiO2-doped elastomer, elastomers being selected due to their wide use in biomaterials. Poly(dimethylsiloxane) (PDMS) was doped with TiO2 nanoparticles and the surface elastomer etched to expose the embedded nanoparticles. As etching results in increased surface roughness, samples with control nanoparticles (SiO2and Fe3O4) were also investigated to decouple the effects of roughness and photoinactivation upon bacterial attachment and inactivation. Characterisation by SEM, AFM and contact angle analysis confirmed that etching produced a rougher (39.3±15.3 vs 5.11±1.29 nm RMS roughness; etched vs unetched TiO2-PDMS), more hydrophobic surface (water contact angle of 120±2.5° vs 110±1.0°; etched TiO2-PDMS vs native PDMS). This surface, rich in exposed photocatalytic TiO2 nanoparticles, allows direct contact between contaminating bacteria and nanoparticles, enabling ROS generation in closer proximity to the bacteria and consequent enhancement of visible light treatment. Incorporating TiO2 into PDMS significantly improved the photoinactivation efficacy (mean bacterial count for light-treated samples normalised to untreated samples of 0.043±0.0081) compared to PDMS alone (0.19±0.036), when seeded with Staphylococcus aureus and exposed to 405 nm, 60 J cm-2 light. However, photoinactivation efficacy was significantly (p<0.001) enhanced by etching the TiO2-PDMS surface (0.015±0.0074), resulting in greater photoinactivation than that obtained for etched (47.0±14.5 nm RMS roughness), non-photocatalytic SiO2-PDMS (0.10±0.093). Results suggest this doping and etching strategy shows significant potential for facilitating decontamination of elastomer-based biomaterials.

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“…Bruening et al reported TiO 2 nanoparticles-decorated porous nylon membrane for rapid phosphopeptide enrichment . Moreover, magnetite-doped polydimethylsiloxane and microfluidic devices supporting TiO 2 nanotube array or TiO 2 –ZrO 2 have also been designed for efficient on-chip enrichment of phosphopeptides through a rapid and straightforward protocol. The microfluidic device supporting TiO 2 nanotube array, for example, realized the efficient enrichment of serum phosphopeptide, which proved the differential expression of serum phosphopeptides between ovarian cancer patients and healthy women.…”

Section: Affinity Materials For the Enrichment Of Phosphorylated Prot...mentioning

confidence: 99%

Development of the Affinity Materials for Phosphorylated Proteins/Peptides Enrichment in Phosphoproteomics Analysis

Wang

et al. 2015

ACS Appl. Mater. Interfaces

147

104

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Reversible protein phosphorylation is a key event in numerous biological processes. Mass spectrometry (MS) is the most powerful analysis tool in modern phosphoproteomics. However, the direct MS analysis of phosphorylated proteins/peptides is still a big challenge because of the low abundance and insufficient ionization of phosphorylated proteins/peptides as well as the suppression effects of nontargets. Enrichment of phosphorylated proteins/peptides by affinity materials from complex biosamples is the most widely used strategy to enhance the MS detection. The demand of efficiently enriching phosphorylated proteins/peptides has spawned diverse affinity materials based on different enrichment principles (e.g., electronic attraction, chelating). In this review, we summarize the recent development of various affinity materials for phosphorylated proteins/peptides enrichment. We will highlight the design and fabrication of these affinity materials, discuss the enrichment mechanisms involved in different affinity materials, and suggest the future challenges and research directions in this field.

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Magnetite-doped polydimethylsiloxane (PDMS) for phosphopeptide enrichment

Cited by 8 publications

References 44 publications

Highly selective enrichment of phosphopeptides by on‐chip indium oxide functionalized magnetic nanoparticles coupled with MALDI‐TOF MS

Highly selective enrichment of phosphopeptides by on‐chip indium oxide functionalized magnetic nanoparticles coupled with MALDI‐TOF MS

Metal oxide-doped elastomeric materials for amplifying visible light-based antimicrobial activity

Development of the Affinity Materials for Phosphorylated Proteins/Peptides Enrichment in Phosphoproteomics Analysis

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