Magnetic-targeting of polyethylenimine-wrapped iron oxide nanoparticle labeled chondrocytes in a rabbit articular cartilage defect model

Abstract

Osteoarthritis (OA) is the most prevalent form of joint disease and lacks effective treatment. Cell-based therapy through intra-articular injection holds great potential for effective intervention at its early stage.Despite the promising outcomes, major barriers for successful clinical application such as lack of specific targeting of transplanted cells still remain. Here, novel polyethylenimine-wrapped iron oxide nanoparticles (PEI/IONs) were utilized as a magnetic agent, and the in vitro efficiency of PEI/ION labeling, and the influence on the chondrogenic properties of chondrocytes were evaluated; the in vivo feasibility of magnetic-targeting intra-articular injection with PEI/ION labeled autologous chondrocytes was investigated using a rabbit articular cartilage defect model. Our data showed that chondrocytes were conveniently labeled with PEI/IONs in a time-and dose-dependent manner, while the viability was unaffected. No significant decrease in collagen type-II synthesis of labeled chondrocytes was observed at low concentration. Macrographic and histology evaluation at 1 week post intra-articular injection revealed efficient cell delivery at chondral defect sites in the magnetic-targeting group. In addition, chondrocytes in the defect area presented a normal morphology, and the origin of cells within was confirmed by immunohistochemistry staining against BrdU and Prussian blue staining. The present study shows proof of concept experiments in magnetic-targeting of PEI/ION labeled chondrocytes for articular cartilage repair, which might provide new insight to improve current cartilage repair strategies.

Pulse electromagnetic fields enhance the repair of rabbit articular cartilage defects with magnetic nano-hydrogel

“…29 Magnetic nanoparticles also have the ability to bind to the cell surface, and the response of magnetic particles under the inuence of external magnetic elds makes it possible to inuence cell differentiation and proliferation. [30][31][32] But we think that the benet effect of magnetic stem cell phenotype needs further external stimulation. By applying external magnetic device, such as PEMFs could stimulate fracture healing and cartilage repair and used as a noninvasive, simple, and side-effect method in the clinical prevention.…”

High-Density Horizontal Stacking of Chondrocytes via the Synergy of Biocompatible Magnetic Gelatin Nanocarriers and Internal Magnetic Navigation for Enhancing Cartilage Repair

“…In the past several years, cell-based therapy has become one of the main strategies for cartilage regeneration. Chondrocytes seeded on biomimetic scaffolds can facilitate cartilage regeneration at damaged sites [ 6 ]; however, the repair results have been not evident due to the difficulty in attaching and proliferating implanted cells at the damaged site in the superficial zone, resulting in the surviving chondrocytes being unable to produce enough cartilage-specific ECM to maintain original biomechanical properties [ 7 ]. As in cell-based therapy for cellular migration and adhesion during tissue healing, targeted delivery of chondrocytes in the superficial zone is a prerequisite for cartilage regeneration.…”

In vitro hyperspectral biomarkers of human chondrosarcoma cells in nanoparticle-mediated radiosensitization using carbon ions

“…Nanoparticle-based drug delivery system combined with radiotherapy could be a promising therapeutic approach. Here, iron oxide nanoparticles are used as doxorubicin carriers owing to their biocompatibility for normal healthy tissue that was demonstrated in clinics [13][14][15][16] and for their magnetic transport capacity 17,18 . In addition, doxorubicin-based chemotherapy proved to be efficient only in some rare cases of CHS due to its systemic toxicity 19 .…”