Magnetic Targeting of Human Olfactory Mucosa Stem Cells Following Intranasal Administration: a Novel Approach to Parkinson’s Disease Treatment

Simorgh, Sara; Bagher, Zohreh; Farhadi, Mohammad; Kamrava, Seyed Kamran; Boroujeni, Mahdi Eskandarian; Namjoo, Zeinab; Hour, Farshid Qiyami; Moradi, Salah; Alizadeh, Rafieh

doi:10.1007/s12035-021-02392-z

Cited by 23 publications

(18 citation statements)

References 46 publications

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“…Next, the tissue suspension was centrifuged (1800 rpm, 5 min), and the supernatant was removed. After several times repeated this step, the cell pellet was cultured in 25 cm 2 flasks consisting of 10% FBS in DMEM/F12 medium, incubated at 37°C with 5% CO 2 , and the medium was replaced twice a week 38 , 39 . The cells were passaged to reach P3.…”

Section: Methodsmentioning

confidence: 99%

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An additive manufacturing‐based3Dprinted poly ɛ‐caprolactone/alginate sulfate/extracellular matrix construct for nasal cartilage regeneration

Zare

Pezeshki‐Modaress

Davachi

et al. 2022

J Biomedical Materials Res

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Various composite scaffolds with different fabrication techniques have been applied in cartilage tissue engineering. In this study, poly ϵ-caprolactone (PCL) was printed by fused deposition modeling method, and the prepared scaffold was filled with Alginate (Alg): Alginate-Sulfate (Alg-Sul) hydrogel to provide a better biomimetic environment and emulate the structure of glycosaminoglycans properly. Furthermore, to enhance chondrogenesis, different concentrations of decellularized extracellular matrix (dECM) were added to the hydrogel. For cellular analyses, the adipose-derived mesenchymal stem cells were seeded on the hydrogel and the results of MTT assay, live/ dead staining, and SEM images revealed that the scaffold with 1% dECM had better viscosity, cell viability, and proliferation. The study was conducted on the optimized scaffold (1% dECM) to determine mechanical characteristics, chondrogenic differentiation, and results demonstrated that the scaffold showed mechanical similarity to the native nasal cartilage tissue along with possessing appropriate biochemical features, which makes this new formulation based on PCL/dECM/Alg:Alg-Sul a promising candidate for further in-vivo studies.

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Section: Methodsmentioning

confidence: 99%

“…medium, incubated at 37 C with 5% CO 2 , and the medium was replaced twice a week. 38,39 The cells were passaged to reach P3. The expression of surface markers of AD-MSCs was tested with the flow cytometry technique to confirm that the harvested cells were stem cells.…”

Section: Preparation Of the 3d-printed Composite Scaffoldmentioning

confidence: 99%

An additive manufacturing‐based3Dprinted poly ɛ‐caprolactone/alginate sulfate/extracellular matrix construct for nasal cartilage regeneration

Zare

Pezeshki‐Modaress

Davachi

et al. 2022

J Biomedical Materials Res

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“…Similarly, in experimental autoimmune encephalomyelitis (EAE) models, IN delivery showed a superior in lesion site retention compared with intraperitoneal or intravenous injection and CXCR4 overexpression further enhanced retention of MSCs 78 , 146 . In 6-OHDA-induced PD model, stem cells or EVs were found to gradually accumulate to the striatum and substantia nigra 147 , stem cells or EVs could still be observed months after administration 138 . Meanwhile, the stem cells or EVs in other brain regions, like the olfactory bulb or brainstem, were constantly decreased after administration 136 .…”

Section: Benefits Of Combining Stem Cells or Evs-based Therapy With I...mentioning

confidence: 98%

“… 123 PD model rats Single administration Behavioral performances were improved one week after administration and the improvement remained to 4 weeks. 147 Ischemic stroke and refusion model mice 1 h after modeling, twice a day for 7 days Inflammation was inhibited and histological structure was restored at end of treatment. Behavioral performances were continuously improved during 7-days-treatment.…”

Section: Benefits Of Combining Stem Cells or Evs-based Therapy With I...mentioning

confidence: 99%

New idea to promote the clinical applications of stem cells or their extracellular vesicles in central nervous system disorders: Combining with intranasal delivery

Jiang

et al. 2022

Acta Pharmaceutica Sinica B

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“…7,35 Olfactory ectomesenchymal stem cells (OE-MSCs) are a new population of neural crest-derived stem cells obtained from lamina propria of the nasal mucosa by non-invasive methods. 36,37 EMSCs possess the multilineage differentiation and self-renewal capacity and a higher potential than the other sources of stem cells to proliferate and differentiate to neurogenic cells, including SCs. [38][39][40] Also, these stem cells have an immunoregulatory function 41 and lack apoptotic and tumorigenic activities [42][43][44] ; therefore, they can be considered an autologous favorable source for cell therapy in PNIs.…”

Section: Introductionmentioning

confidence: 99%

Three‐dimensional‐printed polycaprolactone/polypyrrole conducting scaffolds for differentiation of human olfactory ecto‐mesenchymal stem cells into Schwann cell‐like phenotypes and promotion of neurite outgrowth

Entezari

Mozafari

Bakhtiyari

et al. 2022

J Biomedical Materials Res

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Implantation of a suitable nerve guide conduit (NGC) seeded with sufficient Schwann cells (SCs) is required to improve peripheral nerve regeneration efficiently. Given the limitations of isolating and culturing SCs, using various sources of stem cells, including mesenchymal stem cells (MSCs) obtained from nasal olfactory mucosa, can be desirable. Olfactory ecto-MSCs (OE-MSCs) are a new population of neural crestderived stem cells that can proliferate and differentiate into SCs and can be considered a promising autologous alternative to produce SCs. Regardless, a biomimetic physicochemical microenvironment in NGC such as electroconductive substrate can affect the fate of transplanted stem cells, including differentiation toward SCs and nerve regeneration. Therefore, in this study, the effect of 3D printed polycaprolactone (PCL)/polypyrrole (PPy) conductive scaffolds on differentiation of human OE-MSCS into functional SC-like phenotypes was investigated. Biological evaluation of 3D printed scaffolds was examined by in vitro culturing the OE-MSCs on samples surfaces, and conductivity showed no effect on increased cell attachment, proliferation rate, viability, and distribution. In contrast, immunocytochemical staining and real-time polymerase chain reaction analysis indicated that 3D structures coated with PPy could provide a favorable microenvironment for OE-MSCs differentiation. In addition, it was found that differentiated OE-MSCs within PCL/PPy could secrete the highest amounts of nerve growth factor and brain-derived neurotrophic factor neurotrophic factors compared to pure PCL and 2D culture. After co-culturing with PC12 cells, a significant increase in neurite outgrowth on PCL/PPy conductive scaffold seeded with differentiated OE-MSCs. These findings indicated that 3D printed PCL/PPy conductive scaffold could support differentiation of OE-MSCs into SC-like phenotypes to promote neurite outgrowth, suggesting their potential for neural tissue engineering applications.

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Magnetic Targeting of Human Olfactory Mucosa Stem Cells Following Intranasal Administration: a Novel Approach to Parkinson’s Disease Treatment

Cited by 23 publications

References 46 publications

An additive manufacturing‐based3Dprinted poly ɛ‐caprolactone/alginate sulfate/extracellular matrix construct for nasal cartilage regeneration

An additive manufacturing‐based3Dprinted poly ɛ‐caprolactone/alginate sulfate/extracellular matrix construct for nasal cartilage regeneration

New idea to promote the clinical applications of stem cells or their extracellular vesicles in central nervous system disorders: Combining with intranasal delivery

Three‐dimensional‐printed polycaprolactone/polypyrrole conducting scaffolds for differentiation of human olfactory ecto‐mesenchymal stem cells into Schwann cell‐like phenotypes and promotion of neurite outgrowth

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