Magnetic targeting of bone marrow stromal cells into spinal cord: through cerebrospinal fluid

Nishida, Koji; Tanaka, Nobuhiro; Nakanishi, Kazuyoshi; Kamei, Naosuke; Hamasaki, Takahiko; Yanada, Shinobu; Mochizuki, Yu; Ochi, Mitsuo

doi:10.1097/01.wnr.0000227993.07799.a2

Cited by 59 publications

(49 citation statements)

References 16 publications

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“…The cells circulating in the subarachnoid space were gathered under the magnetic implant. This correlates well with the study of Nishida et al 26 These authors measured the area of GFPpositive cells in the healthy spinal cord under a magnetic implant (5 mm in diameter, 3 mm in height, 350 mT) after the administration of SPION-labeled cells via lumbar puncture and reported a higher concentration of cells in the subarachnoid space below the magnetic implant one day after intrathecal transplantation. In contrast to Nishida et al, we directly counted the number of implanted cells instead of measuring the GFP-positive area, a more accurate approach to determining the number of cells and the cell distribution in the lesion site.…”

Section: Discussionsupporting

confidence: 90%

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Highly efficient magnetic targeting of mesenchymal stem cells in spinal cord injury

Vaněček¹,

Zablotskii²,

Forostyak³

et al. 2012

IJN

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Abstract:The transplantation of mesenchymal stem cells (MSC) is currently under study as a therapeutic approach for spinal cord injury, and the number of transplanted cells that reach the lesioned tissue is one of the critical parameters. In this study, intrathecally transplanted cells labeled with superparamagnetic iron oxide nanoparticles were guided by a magnetic field and successfully targeted near the lesion site in the rat spinal cord. Magnetic resonance imaging and histological analysis revealed significant differences in cell numbers and cell distribution near the lesion site under the magnet in comparison to control groups. The cell distribution correlated well with the calculated distribution of magnetic forces exerted on the transplanted cells in the subarachnoid space and lesion site. The kinetics of the cells' accumulation near the lesion site is described within the framework of a mathematical model that reveals those parameters critical for cell targeting and suggests ways to enhance the efficiency of magnetic cell delivery. In particular, we show that the targeting efficiency can be increased by using magnets that produce spatially modulated stray fields. Such magnetic systems with tunable geometric parameters may provide the additional level of control needed to enhance the efficiency of stem cell delivery in spinal cord injury.

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Section: Discussionsupporting

confidence: 90%

“…Previous studies in this area have focused on targeting cells into the liver 24 or injured brain 25 or have evaluated the homing of cells into the healthy spinal cord with the use of a magnetic system based on a permanent magnet. 26 However, a more detailed evaluation of such magnetic systems is often missing in the literature.…”

Section: Introductionmentioning

confidence: 99%

Highly efficient magnetic targeting of mesenchymal stem cells in spinal cord injury

Vaněček¹,

Zablotskii²,

Forostyak³

et al. 2012

IJN

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“…15,[71][72][73] The data and images shown in Figure 1 illustrate the high proportion of bone marrowderived mesenchymal stem cells labeled with SPIONs. In addition to the stem cell tracking, SPIONs have also been used to magnetically target stem cells to lesion sites, as described by Nishida et al 77 Hamasaki et al, 78 successfully showed, in organotypic cultures, the possibility of enhancing axon formation by magnetically localizing SPION-labeled cells. Studies on the use of SPIONs to focus the delivery of stem cells to specific areas are still being conducted, and have been published by Cheng et al 79 …”

Section: Tracking Stem Cells With Superparamagnetic Iron Oxide Nanopamentioning

confidence: 99%

Tracking stem cells with superparamagnetic iron oxide nanoparticles: perspectives and considerations

Jasmin¹,

Souza²,

Louzada³

et al. 2017

IJN

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Superparamagnetic iron oxide nanoparticles (SPIONs) have been used for diagnoses in biomedical applications, due to their unique properties and their apparent safety for humans. In general, SPIONs do not seem to produce cell damage, although their long-term in vivo effects continue to be investigated. The possibility of efficiently labeling cells with these magnetic nanoparticles has stimulated their use to noninvasively track cells by magnetic resonance imaging after transplantation. SPIONs are attracting increasing attention and are one of the preferred methods for cell labeling and tracking in preclinical and clinical studies. For clinical protocol approval of magnetic-labeled cell tracking, it is essential to expand our knowledge of the time course of SPIONs after cell incorporation and transplantation. This review focuses on the recent advances in tracking SPION-labeled stem cells, analyzing the possibilities and limitations of their use, not only focusing on myocardial infarction but also discussing other models.

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“…In the NP and NP + MF groups, a 2 mm sample of 25 µg/mL IONPs embedded in 3% agarose hydrogel was implanted at the site of injury during surgery, whereas only gel was implanted in the SCI and MF groups. 37,38 The rats were injected subcutaneously with 5 mL of saline to prevent dehydration and injected intramuscularly with gentamicin-cefazolin 20 mg/kg for five days following surgery. 39 The urinary bladder was manually evacuated thrice daily until spontaneous voiding was achieved.…”

Section: Surgerymentioning

confidence: 99%

Iron oxide nanoparticles and magnetic field exposure promote functional recovery by attenuating free radical-induced damage in rats with spinal cord transection

Pal¹,

Singh²,

Nag³

et al. 2013

IJN

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Background: Iron oxide nanoparticles (IONPs) can attenuate oxidative stress in a neutral pH environment in vitro. In combination with an external electromagnetic field, they can also facilitate axon regeneration. The present study demonstrates the in vivo potential of IONPs to recover functional deficits in rats with complete spinal cord injury. Methods: The spinal cord was completely transected at the T11 vertebra in male albino Wistar rats. Iron oxide nanoparticle solution (25 µg/mL) embedded in 3% agarose gel was implanted at the site of transection, which was subsequently exposed to an electromagnetic field (50 Hz, 17.96 µT for two hours daily for five weeks). Results: Locomotor and sensorimotor assessment as well as histological analysis demonstrated significant functional recovery and a reduction in lesion volume in rats with IONP implantation and exposure to an electromagnetic field. No collagenous scar was observed and IONPs were localized intracellularly in the immediate vicinity of the lesion. Further, in vitro experiments to explore the cytotoxic effects of IONPs showed no effect on cell survival. However, a significant decrease in H 2 O 2 -mediated oxidative stress was evident in the medium containing IONPs, indicating their free radical scavenging properties. Conclusion: These novel findings indicate a therapeutic role for IONPs in spinal cord injury and other neurodegenerative disorders mediated by reactive oxygen species.

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Magnetic targeting of bone marrow stromal cells into spinal cord: through cerebrospinal fluid

Cited by 59 publications

References 16 publications

Highly efficient magnetic targeting of mesenchymal stem cells in spinal cord injury

Highly efficient magnetic targeting of mesenchymal stem cells in spinal cord injury

Tracking stem cells with superparamagnetic iron oxide nanoparticles: perspectives and considerations

Iron oxide nanoparticles and magnetic field exposure promote functional recovery by attenuating free radical-induced damage in rats with spinal cord transection

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