Magnetic Susceptibility Measurements of Single Small Particles

Gill, Stanley J.; Malone, C. P.; Downing, M.

doi:10.1063/1.1716878

Cited by 34 publications

(16 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In conclusion, a method has been researched for the simultaneous determination of particle count, particle diameter, and magnetophoretic mobility using dark-field videomicroscope velocimetry with image storage, processing, and analysis software using the commercial Hyperflux particletracking velocimeter (30). This study has demonstrated an available capability to analyze magnetic particles and cells that has been heretofore limited to only a handful of laboratories (15,18,24,(31)(32)(33)(34) with one-of-a-kind instruments. With correct operator-based settings, measurements of magnetophoretic mobility and particle count are robust, and diameter can be determined on the basis of calibration.…”

Section: Resultsmentioning

confidence: 99%

Magnetic particle characterization—magnetophoretic mobility and particle size

et al. 2016

View full text Add to dashboard Cite

Quantitative characterization of magnetic particles is useful for analysis and separation of labeled cells and magnetic particles. A particle velocimeter is used to directly measure the magnetophoretic mobility, size, and other parameters of magnetic particle suspensions. The instrument provides quantitative video analysis of particles and their motion. The trajectories of magnetic particles in an isodynamic magnetic field are recorded using a high-definition camera/microscope system for image collection. Image analysis software then converts the image data to the parameters of interest. The distribution of magnetophoretic mobility is determined by combining fast image analysis with velocimetry measurements. Particle size distributions have been characterized to provide a better understanding of sample quality. The results have been used in the development and operation of analyzer protocols for counting particle concentrations accurately and measuring magnetic susceptibility and size for simultaneous display for routine application to particle suspensions and magnetically labeled biological cells. V C 2016 International Society for Advancement of Cytometry

show abstract

Section: Resultsmentioning

confidence: 99%

Magnetic particle characterization—magnetophoretic mobility and particle size

et al. 2016

View full text Add to dashboard Cite

show abstract

“…With this apparatus, measurements were made of polystyrene latex beads and red blood cells. Winto-Morbach et al (1994, 1995 developed a technique which they refer to as "magneto-cytometry," which is similar to the technique of Gill et al (1960) In this technique, erythrocytes (paramagnetic) or magnetically labeled human leukemia cells (REH and K562), contained within a small capillary tube, are placed in a high magnetic field gradient created by an electromagnet. By microscopically determining the number of cells disappearing from the field of view after turning on the magnet, the fraction of the magnetic cells can be determined.…”

Section: Quantification Of Magnetic Susceptibilitymentioning

confidence: 99%

“…A typical FACS histogram contains the results of 10 4 cells. Consequently, without computer automation, the use of the magneto-cytometry device and that of Gill et al (1960) is limited to a few hundred cells (Winto-Morbach, 1996, 1995. Third, in the reports on the magneto-cytometer device, the authors do not report values for the 1 ⁄2ٌB 2 .…”

Section: Quantification Of Magnetic Susceptibilitymentioning

confidence: 99%

Quantification of cellular properties from external fields and resulting induced velocity: Magnetic susceptibility

Chalmers¹,

Haam²,

Zhao³

et al. 1999

Biotechnol. Bioeng.

View full text Add to dashboard Cite

An experimental technique is discussed in which the magnetic susceptibility of immunomagnetically labeled cells can be determined on a cell‐by‐cell basis. This technique is based on determining the magnetically induced velocity that an immunomagnetically labeled cell has in a well‐defined magnetic energy gradient. This velocity is determined through the use of video recordings of microscopic images of cells moving in the magnetic energy gradient. These video images are then computer digitized and processed using a computer algorithm, cell tracking velocimetry, which allows larger numbers (>103) of cells to be analyzed. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 64:519–526, 1999.

show abstract

“…Characterization and separation of submagnetic microparticles, especially biological particles, with the use of their intrinsic magnetic differences, had been attempted repeatedly in the past, [1][2][3][4][5][6] and remains the subject of noticeable interest. [7][8][9][10][11][12][13][14] As a rule, assessing magnetic properties of such particles relies on the indirect methods employing characteristics of the recorded particle motion in a liquid under the action of a high-gradient magnetic field.…”

Section: Introductionmentioning

confidence: 99%

Magnetophoretic trajectory tracking magnetometry: A new technique for assessing magnetic properties of submagnetic microparticles and cells

Kashevsky

Zholud

Kashevsky

2012

Review of Scientific Instruments

View full text Add to dashboard Cite

We develop a new approach for assessing magnetic properties of submagnetic microparticles and cells, magnetophoretic trajectory tracking magnetometry (MTTM), that employs recording of long 2D trajectories of particle motion in a slot fluid channel caused by the action of crossed gravitational and magnetic forces. The studies are focused on the development of theoretical backgrounds of the method and evaluation of its uncertainty caused by the mutual hydrodynamic entrainment of moving particles. Computerized equipment implementing MTTM technique is described and its performance is illustrated. According to our studies, the new technique can serve a reliable experimental method with analytical qualities.

show abstract

Magnetic Susceptibility Measurements of Single Small Particles

Cited by 34 publications

References 2 publications

Magnetic particle characterization—magnetophoretic mobility and particle size

Magnetic particle characterization—magnetophoretic mobility and particle size

Quantification of cellular properties from external fields and resulting induced velocity: Magnetic susceptibility

Magnetophoretic trajectory tracking magnetometry: A new technique for assessing magnetic properties of submagnetic microparticles and cells

Contact Info

Product

Resources

About