Magnetic/Silica Nanocomposites as Dual‐Mode Contrast Agents for Combined Magnetic Resonance Imaging and Ultrasonography

Malvindi, Maria Ada; Greco, Antonio; Conversano, Francesco; Figuerola, Albert; Corti, M.; Bonora, Marco; Lascialfari, A.; Doumari, Houshang Amiri; Moscardini, M.; Cingolani, R.; Gigli, Giuseppe; Casciaro, Sergio; Pellegrino, Teresa; Ragusa, Andrea

doi:10.1002/adfm.201100031

Cited by 87 publications

(70 citation statements)

References 35 publications

(11 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[9][10][11] These silica-coated fluorescent magnetic nanoprobes enabled development of biomedical platforms for simultaneous imaging, diagnosis, and therapy. [12][13][14] However, it is still a challenge to fabricate fluorescent magnetic nanoprobes with high photostability, high payloads of dye, and desirable outer surfaces for further modification with functional or target moieties.…”

Section: Introductionmentioning

confidence: 99%

VCAM-1-targeted core/shell nanoparticles for selective adhesion and delivery to endothelial cells with lipopolysaccharide-induced inflammation under shear flow and cellular magnetic resonance imaging in vitro

Yang¹,

Zhao²,

Liu³

et al. 2013

IJN

View full text Add to dashboard Cite

Multifunctional nanomaterials with unique magnetic and luminescent properties have broad potential in biological applications. Because of the overexpression of vascular cell adhesion molecule-1 (VCAM-1) receptors in inflammatory endothelial cells as compared with normal endothelial cells, an anti-VCAM-1 monoclonal antibody can be used as a targeting ligand. Herein we describe the development of multifunctional core-shell Fe 3 O 4 @SiO 2 nanoparticles with the ability to target inflammatory endothelial cells via VCAM-1, magnetism, and fluorescence imaging, with efficient magnetic resonance imaging contrast characteristics. Superparamagnetic iron oxide and fluorescein isothiocyanate (FITC) were loaded successfully inside the nanoparticle core and the silica shell, respectively, creating VCAM-1-targeted Fe 3 O 4 @ SiO 2 (FITC) nanoparticles that were characterized by scanning electron microscopy, transmission electron microscopy, fluorescence spectrometry, zeta potential assay, and fluorescence microscopy. The VCAM-1-targeted Fe 3 O 4 @SiO 2 (FITC) nanoparticles typically had a diameter of 355 ± 37 nm, showed superparamagnetic behavior at room temperature, and cumulative and targeted adhesion to an inflammatory subline of human umbilical vein endothelial cells (HUVEC-CS) activated by lipopolysaccharide. Further, our data show that adhesion of VCAM-1-targeted Fe 3 O 4 @SiO 2 (FITC) nanoparticles to inflammatory HUVEC-CS depended on both shear stress and duration of exposure to stress. Analysis of internalization into HUVEC-CS showed that the efficiency of delivery of VCAM-1-targeted Fe 3 O 4 @SiO 2 (FITC) nanoparticles was also significantly greater than that of nontargeted Fe 3 O 4 @SiO 2 (FITC)-NH 2 nanoparticles. Magnetic resonance images showed that the superparamagnetic iron oxide cores of the VCAM-1-targeted Fe 3 O 4 @SiO 2 (FITC) nanoparticles could also act as a contrast agent for magnetic resonance imaging. Taken together, the cumulative adhesion and uptake potential of these VCAM-1-targeted Fe 3 O 4 @SiO 2 (FITC) nanoparticles targeted to inflammatory endothelial cells could be used in the transfer of therapeutic drugs/genes into these cells or for diagnosis of vascular disease at the molecular and cellular levels in the future.

show abstract

Section: Introductionmentioning

confidence: 99%

VCAM-1-targeted core/shell nanoparticles for selective adhesion and delivery to endothelial cells with lipopolysaccharide-induced inflammation under shear flow and cellular magnetic resonance imaging in vitro

Yang¹,

Zhao²,

Liu³

et al. 2013

IJN

View full text Add to dashboard Cite

show abstract

“…Very recently, a facilely available structure design concept on UCAs -double scattering/reflection in a single nanoparticle for intensifying US imaging has been proposed and experimentally and theoretically 24 Moreover, another available structure design concept that is adding some nanoparticles in shell of UCAs to improve ultrasound imaging via promoting nonlinear scattering has been also experimentally accepted. [25][26][27] Inspired by this, we incorporated the principles of above two structure design concepts, and further integrate them into design of US&CT&MR trimodal imaging probe, and the special structure of as-prepared Au@HMSN/Au&MnO indeed perfectly caters to above-mentioned two structure design concepts. As shown in Figure 3d, the large Au nanoparticle in the hollow cavity of HMSNs can expectedly provide the second interface to promote occurrence of double scattering/reflection together with outer surface of integral particle (A zone), while some lots of smaller Au nanocrystals and MnO nanoparticles are expected to enhance nonlinear scattering (B zone), i.e., promoting emergence of ultraharmonics (2ƒ 0 , 3ƒ 0 , et al) and subharmonics (ƒ 0 /2), both of which will expectedly improve US imaging ability of this trimodal imaging probe.…”

Section: Structural Superiority Of Au@hmsn/auandmno In Intensifying Us mentioning

confidence: 97%

Marriage Strategy of Structure and Composition Designs for Intensifying Ultrasound & MR & CT Trimodal Contrast Imaging

Zhang

Chen

et al. 2015

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Despite great efforts having been devoted to the design of multimodal imaging probe, almost all design principles of nanotheranostic agents subordinate to simple assemblies of building blocks, resulting in complex preparation process and discounted ability, that is, 1 + 1 < 2. In this report, a novel design strategy, marriage of structure design and composition design that can maximize imaging ability of each building block, ultimately achieving 1 + 1 ≥ 2, has been established. Moreover, a high-efficient ultrasound (US) & MR & CT trimodal contrast agent acts as model to instantiate this design strategy, wherein nanoparticles-induced nonlinear scattering and rattle-type structure-induced double scattering enhancing US imaging, and uniform distribution of Mn(2+) paramagentic centers and "core-satellite" structure of Au atoms favoring enhanced MR imaging and CT imaging, respectively have been validated, achieving optimization of structure design. Importantly, the selected components, silica, Au and MnO are endowed with excellent biocompatibility, displaying the marriage strategy of composition design with aforementioned structure optimization. In in vivo evaluations, such a biocompatible trimodal probe is demonstrated of excellent performance in intensifying CT, MR and US imaging in vivo, especially after positively charged modification by PEI promoting more probes retained in tumor. More importantly, as a universal design strategy, the involved principles in constructing such a US&MR&CT trimodal imaging probe promise great potentials in guiding designs of other materials-based multimodal imaging probe.

show abstract

“…Recently, nanotechnology has been shown to be able to provide valuable tools for potentially diagnosing and treating diseases in many fields of medicine, including NDs [4][5][6][7][8][9][10]. Furthermore, NPs can help to overcome several limitations of traditional drugs by improving their bioavailability and half-life, yielding a sustained release, in addition to allowing specific targeting [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Neuroprotective Investigation of Chitosan Nanoparticles for Dopamine Delivery

et al. 2018

Self Cite

View full text Add to dashboard Cite

Featured Application: Chitosan nanoparticles are potential candidates for the delivery of various drugs, including dopamine.Abstract: Chitosan nanoparticles (CS NPs) have been widely exploited for the delivery of various types of drugs due to their biocompatibility, availability, ease of functionalization and other advantages. Nevertheless, despite their wide use, their mechanism of action is not very clear and many aspects still need to be investigated in detail, with only a few studies having studied the behavior of this polymer. We prepared CS NPs encapsulating dopamine (DA) and studied the generation of reactive oxygen species (ROS) and the antioxidant effect of the neurotransmitter in detail. Encapsulation of the drug and its subsequent sustained release significantly reduced the oxidation rate in vitro, thus potentially exerting neuroprotective effects. ROS production in SH-SY5Y cells was investigated through a H 2 O 2 assay, while a deeper study of the enzymatic activity allowed us to determine the significant contribution of both GPx and SOD enzymes in preventing oxidative stress.

show abstract

Magnetic/Silica Nanocomposites as Dual‐Mode Contrast Agents for Combined Magnetic Resonance Imaging and Ultrasonography

Cited by 87 publications

References 35 publications

VCAM-1-targeted core/shell nanoparticles for selective adhesion and delivery to endothelial cells with lipopolysaccharide-induced inflammation under shear flow and cellular magnetic resonance imaging in vitro

VCAM-1-targeted core/shell nanoparticles for selective adhesion and delivery to endothelial cells with lipopolysaccharide-induced inflammation under shear flow and cellular magnetic resonance imaging in vitro

Marriage Strategy of Structure and Composition Designs for Intensifying Ultrasound & MR & CT Trimodal Contrast Imaging

Neuroprotective Investigation of Chitosan Nanoparticles for Dopamine Delivery

Contact Info

Product

Resources

About