Magnetic Resonance Detection of CD34+ Cells from Umbilical Cord Blood Using a 19F Label

Abstract

Impaired homing and delayed recovery upon hematopoietic stem cell transplantation (HSCT) with hematopoietic stem cells (HSC) derived from umbilical cord blood (UCB) is a major problem. Tracking transplanted cells in vivo will be helpful to detect impaired homing at an early stage and allows early interventions to improve engraftment and outcome after transplantation. In this study, we show sufficient intracellular labeling of UCB-derived CD34+ cells, with 19F-containing PLGA nanoparticles which were detectable with both flow cytometry and magnetic resonance spectroscopy (MRS). In addition, labeled CD34+ cells maintain their capacity to proliferate and differentiate, which is pivotal for successful engraftment after transplantation in vivo. These results set the stage for in vivo tracking experiments, through which the homing efficiency of transplanted cells can be studied.

Transient loading of CD34⁺ hematopoietic progenitor cells with polystyrene nanoparticles

“…28 Duinhouwer et al showed the uptake of PGLA NPs in HPCs after 4 and 20 hours of exposure, without affecting their proliferation and differentiation capabilities. 29 Other studies in which iron oxide and Gd 2 O 3 NPs by HPCs were used for MRI applications have reported solely their uptake and little effect on cellular function. [9][10][11]30,31 None of these preceding studies have reported the short-time kinetics of NPs in HPCs.…”

Fluorine-19 Magnetic Resonance Imaging and Positron Emission Tomography of Tumor-Associated Macrophages and Tumor Metabolism

“…Another concern is that fluorination of TAMs with PFC nanoemulsions might affect the phenotype and physiology of these cells. In several studies, PFC nanoemulsions have been shown to preserve the original function and differentiation potential of various cell types, including hematopoietic [ 33 , 34 ], neural [ 35 ], and mouse-mesenchymal [ 36 ] stem cells. Similar studies should be conducted to investigate the influence of PFC labeling on TAM characteristics.…”

Delivery of RNA-based molecules to human hematopoietic stem and progenitor cells for modulation of gene expression

“…The cellular uptake occurs via endocytosis as well, and the endosomal release is mediated by electrostatic interaction with the endosomal membrane [60]. PLGA nanoparticles have successfully been used for gene correction [61] as well as for intracellular labeling of HSPCs with 19 F [62]. However, culture of HSPCs with plasmidor small interfering RNA (siRNA)-loaded nanoparticles results in relatively low gene expression and knockdown efficiencies, respectively [61,63], presumably because of insufficient uptake.…”