Magnetic poly ε-caprolactone nanoparticles containing Fe₃O₄and gemcitabine enhance anti-tumor effect in pancreatic cancer xenograft mouse model

Abstract

We prepared magnetic (Fe(3)O(4)) poly epsilon-caprolactone (PCL) nanoparticles (mean diameter 164 +/- 3 nm) containing an anticancer drug (gemcitabine) using emulsion-diffusion method in order to develop more efficient drug delivery for cancer treatment. Nanoparticles were smooth, well individualized and homogeneous in size. The values of magnetizations for the magnetic PCL nanoparticles were observed around 10.2 emu/g at 2000 Oe magnetic field intensity and showed super-paramagnetic property. In case of the drug, the drug loading contents was 18.6% and entrapment efficiency was 52.2%. The anti-tumor effects caused by these particles were examined using nude mice bearing subcutaneous human pancreatic adenocarcinoma cells (HPAC) in vivo. We divided that these mice were randomly assigned to one of five treatment groups for experimental contrast. The antitumor effect was showed with 15-fold higher dose when compared to free gemcitabine. From the result, the magnetic PCL nanoparticles may provide a therapeutic benefit by delivering drugs efficiently to magnetically targeted tumor tissues, thus achieving safe and successful anti-tumor effects with low toxicity.

Preparation of albumin nanospheres loaded with gemcitabine and their cytotoxicity against BXPC-3 cells in vitro

“…Drug loadings were 11.25% and 13.40%; drug encapsulation rates were 82.92% and 92.56%, respectively. These parameters were somewhat higher than in other kinds of drug-loaded nanoparticles prepared analogously [13][14][15][16][17][18][19] . Zeta potentials were -24.4 and -15.6 mV, respec- Figure 1).…”

“…Albumin is a safe, nontoxic, biocompatible, and biodegradable water-soluble protein present in human plasma [10][11][12] . No problems with hemolysis or immunogenicity have been associated with albumin, in contrast to artificial npg materials such as polycaprolactone, polycyanoacrylate, or polybutylcyanoacrylate [13][14][15][16][17][18][19] . Although these compounds are also biodegradable, their safety following intravenous injection has not been confirmed.…”

Chitosan and Glyceryl Monooleate Nanostructures Containing Gemcitabine: Potential Delivery System for Pancreatic Cancer Treatment

“…Nanoparticle based delivery system using Cetuximab as a targeting agent has been developed and tested in the treatment of pancreatic cancer (15). Gang and coworkers have shown the effectiveness of magnetic poly epsiloncaprolactone nanostructure-containing ferric oxide and gemcitabine in the treatment of pancreatic cancer in pancreatic cancer xenografts mouse model (16). Stella and coworkers evaluated the use of polycyanoacrylate to fabricate nanoparticles for a lipophilic conjugate of gemcitabine.…”

Targeted magnetic iron oxide nanoparticles for tumor imaging and therapy

“…Increasing evidence shows that the selective delivery of therapeutic agents into a tumor mass may minimize toxicity to normal tissues and improve bioavailability of cytotoxic agents (Shenoy et al 2005;Gang et al 2007;Bae et al 2007;Lee et al 2007c).…”