Chitosan and Glyceryl Monooleate Nanostructures Containing Gemcitabine: Potential Delivery System for Pancreatic Cancer Treatment

Trickler, William J.; Khurana, Jatin; Nagvekar, Ankita A.; Dash, Alekha K.

doi:10.1208/s12249-010-9393-0

Cited by 43 publications

(33 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There was no significant (p<0.05) difference observed in the particle size of drug-loaded GMOchitosan and PLGA nanoparticles. In contrast to the previous studies with GMO-chitosan nanoparticles in our lab (20,21), the present study produced particles of smaller size. The Values are mean±SD; n=3 n/d not defined, PTX paclitaxel, CYP cyclopamine, EE entrapment efficiency difference could possibly be attributed to the use of poloxamer as a stabilizer instead of PVA.…”

Section: Nanoparticle Characterizationcontrasting

confidence: 96%

“…Our laboratory was previously involved in the development of GMO-chitosan nanoparticles for the delivery of hydrophilic as well as hydrophobic anticancer agents utilizing the bioadhesive properties of chitosan and GMO for targeting the cancer tissue (20,21). However, in the previous studies, we have only reported the targeting of drugs individually and not in combination.…”

Section: Discussionmentioning

confidence: 99%

“…Nanoparticles are known to show an increase in the intracellular drug concentration via energy-dependent processes of internalization like endocytosis (21,29,30). GMOchitosan nanoparticles have shown to possess bioadhesion properties (20).…”

Section: The Cellular Association and Subcellular Localizationmentioning

confidence: 99%

See 2 more Smart Citations

Multifunctional Nanoparticles for Prostate Cancer Therapy

Chandratre

Dash

2014

AAPS PharmSciTech

Self Cite

View full text Add to dashboard Cite

Abstract. The relapse of cancer after first line therapy with anticancer agents is a common occurrence. This recurrence is believed to be due to the presence of a subpopulation of cells called cancer stem cells in the tumor. Therefore, a combination therapy which is susceptible to both types of cells is desirable. Delivery of this combinatorial approach in a nanoparticulate system will provide even a better therapeutic outcome in tumor targeting. The objective of this study was to develop and characterize nanoparticulate system containing two anticancer agents (cyclopamine and paclitaxel) having different susceptibilities toward cancer cells. Both drugs were entrapped in glyceryl monooleate (GMO)-chitosan solid lipid as well as poly(glycolic-lactic) acid (PLGA) nanoparticles. The cytotoxicity studies were performed on DU145, DU145 TXR, and Wi26 A4 cells. The particle size of drug-loaded GMO-chitosan nanoparticles was 278.4 ±16.4 nm with a positive zeta potential. However, the PLGA particles were 234.5±6.8 nm in size with a negative zeta potential. Thermal analyses of both nanoparticles revealed that the drugs were present in noncrystalline state in the matrix. A sustained in vitro release was observed for both the drugs in these nanoparticles. PLGA blank particles showed no cytotoxicity in all the cell lines tested, whereas GMOchitosan blank particles showed substantial cytotoxicity. The types of polymer used for the preparation of nanoparticles played a major role and affected the in vitro release, cytotoxicity, and uptake of nanoparticles in the all the cell lines tested.

show abstract

Section: Nanoparticle Characterizationcontrasting

confidence: 96%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Multifunctional Nanoparticles for Prostate Cancer Therapy

Chandratre

Dash

2014

AAPS PharmSciTech

Self Cite

View full text Add to dashboard Cite

show abstract

“…Chitosan has the higher degree of deacetylation, which makes it more soluble in biological system (Sannan et al 1976). The low-molecular-weight chitosan also helps to achieve smaller particle size and better redispersibiliy of particles in water (Trickler et al 2010). The nanoparticles prepared with chitosan produce positive surface charge.…”

Section: Stability Of Ifosfamide Loaded Nanostructured Lipid Carriermentioning

confidence: 99%

Development and optimization of ifosfamide nanostructured lipid carriers for oral delivery using response surface methodology

Velmurugan

Selvamuthukumar

2015

Appl Nanosci

View full text Add to dashboard Cite

The research focuses on the development and optimization of ifosfamide nanostructured lipid carriers for oral delivery with the application of response surface methodology. The objectives of the study were to develop a formulation for ifosfamide to be delivered orally, overcome the instability of the drug in acidic environment during oral administration, to sustain the release, drug leakage during storage and low loading capacity. A modified solvent diffusion method in aqueous system was applied to prepare nanostructured lipid nanoparticles. Hydrophilic polymers such as chitosan and sodium alginate were used as coating materials. Glycerol mono oleate and oleic acid were used as solid and liquid lipid, respectively. Poloxamer is used as stabilizers. The central composite rotatable design consisting of three-factored factorial design with three levels was used in this study. The physiochemical characterization included evaluation of surface morphology, particle size and surface charge of the drug in the delivery system. The in vitro drug release, entrapment and drug loading efficiency and as well as the storage stability were evaluated. The results showed that the optimal formulation was composed of drug/lipid ratio of 1:3, organic/aqueous phase ratio of 1:10 and concentration of surfactant of 1 % w/v. Ifosfamide nanostructured lipid carrier under the optimized conditions gave rise to the entrapment efficiency of 77 %, drug loading of 6.14 %, mean diameter of 223 nm and zeta potential value of -25 mV. Transmission electron microscopy analysis showed spherical particles. The in vitro experiment proved that ifosfamide from the delivery system released gradually over the period of 72 h. Sodium alginate cross-linked chitosan nanostructured lipid carrier demonstrated enhanced stability of ifosfamide, high entrapment efficiency and sustained release.

show abstract

“…The preparation method developed by Trickler et al was used for the preparation of GMO-chitosan nanoparticles (15). In brief, 1.75 ml of GMO was melted and added to 12.5 ml of 1% w/v poloxamer 407 solution.…”

Section: Preparation Of Gmo-chitosan Nanoparticlesmentioning

confidence: 99%

Physical-Chemical Characterization and Formulation Considerations for Solid Lipid Nanoparticles

et al. 2015

View full text Add to dashboard Cite

Abstract. Pure glyceryl mono-oleate (GMO) (lipid) and different batches of GMO commonly used for the preparation of GMO-chitosan nanoparticles were characterized by modulated differential scanning calorimetry (MDSC), cryo-microscopy, and cryo-X-ray powder diffraction techniques. GMO-chitosan nanoparticles containing poloxamer 407 as a stabilizer in the absence and presence of polymers as crystallization inhibitors were prepared by ultrasonication. The effect of polymers (polyvinyl pyrrolidone (PVP), Eudragits, hydroxyl propyl methyl cellulose (HPMC), polyethylene glycol (PEG)), surfactants (poloxamer), and oils (mineral oil and olive oil) on the crystallization of GMO was investigated. GMO showed an exothermic peak at around −10°C while cooling and another exothermic peak at around −12°C while heating. It was followed by two endothermic peaks between 15 and 30 C, indicative of GMO melting. The results are corroborated by cryo-microscopy and cryo-X-ray. Significant differences in exothermic and endothermic transition were observed between different grades of GMO and pure GMO. GMO-chitosan nanoparticles resulted in a significant increase in particle size after lyophilization. MDSC confirmed that nanoparticles showed similar exothermic crystallization behavior of lipid GMO. MDSC experiments showed that PVP inhibits GMO crystallization and addition of PVP showed no significant increase in particle size of solid lipid nanoparticle (SLN) during lyophilization. The research highlights the importance of extensive physical-chemical characterization for successful formulation of SLN.

show abstract

Chitosan and Glyceryl Monooleate Nanostructures Containing Gemcitabine: Potential Delivery System for Pancreatic Cancer Treatment

Cited by 43 publications

References 41 publications

Multifunctional Nanoparticles for Prostate Cancer Therapy

Multifunctional Nanoparticles for Prostate Cancer Therapy

Development and optimization of ifosfamide nanostructured lipid carriers for oral delivery using response surface methodology

Physical-Chemical Characterization and Formulation Considerations for Solid Lipid Nanoparticles

Contact Info

Product

Resources

About