Magnetic particles–based biosensor for biogenic amines using an optical oxygen sensor as a transducer

Pospíšková, Kristýna; Šafařı́k, Ivo; Šebela, Marek; Kuncová, Gabriela

doi:10.1007/s00604-012-0932-0

Cited by 46 publications

(25 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Special attraction has also been paid to biomedical applications like drug delivery, biosensors, magnetic resonance imaging and magnetic hydrothermia [1][2][3][4][5][6][7]. Manganese ferrite nanoparticles exhibit superior mechanical, luminescent and magnetic properties compared to other existing magnetic ferrite nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Synthesis, Characterization and in Vitro Evaluation of Manganese Ferrite (MnFe2O4) Nanoparticles for Their Biocompatibility with Murine Breast Cancer Cells (4T1)

et al. 2016

View full text Add to dashboard Cite

Manganese ferrite (MnFe 2 O 4 ) magnetic nanoparticles were successfully prepared by a sol-gel self-combustion technique using iron nitrate and manganese nitrate, followed by calcination at 150˝C for 24 h. Calcined sample was systematically characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and vibrational sample magnetometry (VSM) in order to identify the crystalline phase, functional group, morphology, particle size, shape and magnetic behavior. It was observed that the resultant spinal ferrites obtained at low temperature exhibit single phase, nanoparticle size and good magnetic behavior. The study results have revealed the existence of a potent dose dependent cytotoxic effect of MnFe 2 O 4 nanoparticles against 4T1 cell lines at varying concentrations with IC 50 values of 210, 198 and 171 µg/mL after 24 h, 48 h and 72 h of incubation, respectively. Cells exposed to higher concentrations of nanoparticles showed a progressive increase of apoptotic and necrotic activity. Below 125 µg/mL concentration the nanoparticles were biocompatible with 4T1 cells.

show abstract

Section: Introductionmentioning

confidence: 99%

Synthesis, Characterization and in Vitro Evaluation of Manganese Ferrite (MnFe2O4) Nanoparticles for Their Biocompatibility with Murine Breast Cancer Cells (4T1)

et al. 2016

View full text Add to dashboard Cite

show abstract

“…The chemicals and material used in this study, as well as the preparation of a sensitive lens with diamine oxidase (DAO; EC 1.4.3.22) pre-immobilised on magnetite Sepabeads EC-HA400, were as described by Pospiskova et al (2013). With the aim of enhancing the homogeneity of the sensitive layer, magnetite Sepabeads with immobilised DAO were crushed prior to mixing with ORMOCER and deposition on the lens.…”

Section: Methodsmentioning

confidence: 99%

“…In a previous study (Pospiskova et al, 2013) the dark colour of the magnetic particles was demonstrated as not disturbing the responses of a biosensor with an optical oxygen transducer. Without putrescine, the lifetime responses of the biosensor were inversely proportional to the concentrations of dissolved oxygen (Fig.…”

Section: Biosensor Responsesmentioning

confidence: 96%

“…More rugged optodes comprising a mixture of both components were prepared by a co-entrapment of the pre-immobilised enzyme and oxygen sensitive metal complex in chemically and mechanically stable UV-curable ORMOCER (Scully et al, 2007). The durability of such a glucose sensor was assayed in a week-long fermentation process (Kuncová & Šandová, 2006) and a similar sensor for biogenic amines had a limit of detection of 0.03 mmol L −1 of putrescine (Pospiskova et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Enzymatic sensor of putrescine with optical oxygen transducer – mathematical model of responses of sensitive layer

et al. 2015

View full text Add to dashboard Cite

A biosensor for putrescine containing a sensing layer with an optical oxygen probe based on ruthenium complex and the enzyme diamine oxidase from pea is described. The diamine oxidase was pre-immobilised on broken micro-beads modified with a ferrofluid. The pre-immobilised enzyme and ruthenium complex were both incorporated into the UV-cured inorganic-organic hybrid polymer ORMOCER and deposited on a lens to form a sensitive layer of 210 µm in thickness. The sensitivity to the putrescine concentration determined under air saturation was between 3.50 µs L mmol −1 and 4.50 µs L mmol −1 in a hundred experiments conducted intermittently over a one year period. With the oxygen concentration increasing from 10 % to 100 % of DO (dissolved oxygen), the biosensor sensitivity decreased from 6.87 µs L mmol −1 to 0.70 µs L mmol −1 and its dynamic range increased from 0.10 mmol L −1 to 1.75 mmol L −1 . To estimate the behaviour of the putrescine sensor in parametric space, a mathematical model of the reaction-transport processes inside the sensing layer was developed. The model revealed the qualitative relations between the sensor analytical features, the characteristics of the sensitive layer and concentrations of substrates. The results of the mathematical modelling may serve as guidelines in the design of optodes for specific applications.

show abstract

“…Electrochemical techniques have been extensively used to characterize the development of biosensors and to evaluate biomolecular recognition [13,14]. Metal oxide nanoparticles exhibit unique properties, such as increased surface area, magnetism, chemical reduction, ligand sequestration and optical characteristics [15][16][17]. In general, magnetic nanoparticles have advantages such as good susceptibility, dispersibility and the ability to be used in association with other metal nanoparticles to obtain new properties without change in its magnetic properties [18,19].…”

Section: Introductionmentioning

confidence: 99%

Self-assembled monolayers of mercaptobenzoic acid and magnetite nanoparticles as an efficient support for development of tuberculosis genosensor

Costa

Andrade

Montenegro³

et al. 2014

Journal of Colloid and Interface Science

View full text Add to dashboard Cite

In this work, a genosensor for the electrochemical detection of genomic DNA from Mycobacterium tuberculosis was developed. The biosensor is based on self-assembled monolayers of mercaptobenzoic acid (MBA) and magnetite nanoparticles (Fe3O4Nps) on bare gold electrode for immobilization of DNA probe. The aim of this work was the development of a platform based on cysteine-coated magnetic Fe3O4Nps linked via the carboxylate group from MBA to the work electrode surface and subsequently to the DNA probe. The probe-genome interaction was evaluated using a [Fe(CN)6](4-)/[Fe(CN)6](3-) redox pair. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to evaluate the bioelectrochemical behavior of the sensor. Atomic force microscopy images showed Fe3O4Nps immobilized across the electrode surface. The interaction of the sensor with different genome DNA concentrations resulted in changes in the charge transfer resistance, indicating a possible use for tuberculosis detection at low concentrations (detection limit of 6ngμL(-1)).

show abstract

Magnetic particles–based biosensor for biogenic amines using an optical oxygen sensor as a transducer

Cited by 46 publications

References 29 publications

Synthesis, Characterization and in Vitro Evaluation of Manganese Ferrite (MnFe2O4) Nanoparticles for Their Biocompatibility with Murine Breast Cancer Cells (4T1)

Synthesis, Characterization and in Vitro Evaluation of Manganese Ferrite (MnFe2O4) Nanoparticles for Their Biocompatibility with Murine Breast Cancer Cells (4T1)

Enzymatic sensor of putrescine with optical oxygen transducer – mathematical model of responses of sensitive layer

Self-assembled monolayers of mercaptobenzoic acid and magnetite nanoparticles as an efficient support for development of tuberculosis genosensor

Contact Info

Product

Resources

About