After our first discovery of the multi-band superconductivity (SC) in TlNi2Se2 crystal, we grew successfully a series of TlNi2Se2−xSx (0.0 ≤ x ≤ 2.0) single crystals. The measurements of resistivity, specific heat and susceptibility were carried out for them. Superconductivity with T C =2.3K was first observed in TlNi2S2 crystal, which also appears to involve heavy electrons with an effective mass m*=13∼25 m b , as inferred from the normal state electronic specific heat and the upper critical field, H C2(T), respectively. It was found that bulk superconductivity and heavy electron behavior is preserved in all the TlNi2Se2−xSx samples. In the mixed state, a novel change of the field dependence of the residual specific heat coefficient, γN (H), occurs in TlNi2Se2−xSx, with increasing of the S content. We also found that the TC value changes with the disorder degree induced by the partial substitution of S for Se, characterized by the residual resistivity ratio (RRR). Thus, TlNi2Se2−xSx system provides a platform to study the effect of disorder on the multi-band superconductivity.