Magnetic nanoparticles for the manipulation of proteins and cells

Xiao, Xiaohui; Du, Xuewen; Zhao, Fan; Xu, Bing

doi:10.1039/c2cs15315g

Cited by 341 publications

(206 citation statements)

References 160 publications

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“…Several approaches have been developed to produce complex cell patterns, clusters, assembled arrays, and even tissue structures. These approaches use many disparate technologies which include optics, magnetic and electrical fields, injection printing, physical or geometric constraints, or surface engineering (6)(7)(8)(9)(10)(11). However, there is currently a paucity of a single method that can facilitate the formation of complex multicellular structures with high precision, high versatility, multiple dimensionality, and single-cell resolution, while maintaining cell viability, integrity, and function.…”

Section: D Particle Manipulationmentioning

confidence: 99%

Three-dimensional manipulation of single cells using surface acoustic waves

Guo

Mao

Chen

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

461

331

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The ability of surface acoustic waves to trap and manipulate micrometer-scale particles and biological cells has led to many applications involving "acoustic tweezers" in biology, chemistry, engineering, and medicine. Here, we present 3D acoustic tweezers, which use surface acoustic waves to create 3D trapping nodes for the capture and manipulation of microparticles and cells along three mutually orthogonal axes. In this method, we use standing-wave phase shifts to move particles or cells in-plane, whereas the amplitude of acoustic vibrations is used to control particle motion along an orthogonal plane. We demonstrate, through controlled experiments guided by simulations, how acoustic vibrations result in micromanipulations in a microfluidic chamber by invoking physical principles that underlie the formation and regulation of complex, volumetric trapping nodes of particles and biological cells. We further show how 3D acoustic tweezers can be used to pick up, translate, and print single cells and cell assemblies to create 2D and 3D structures in a precise, noninvasive, label-free, and contact-free manner.3D acoustic tweezers | cell printing | 3D cell manipulation | cell assembly | 3D particle manipulationT he ability to precisely manipulate living cells in three dimensions, one cell at a time, offers many possible applications in regenerative medicine, tissue engineering, neuroscience, and biophysics (1-3). However, current bioprinting methods are generally hampered by the need to reconstruct and mimic 3D cellto-cell communications and cell-environment interactions. Because of this constraint, bioprinting requires accurate reproduction of multicellular architecture (4, 5). Several approaches have been developed to produce complex cell patterns, clusters, assembled arrays, and even tissue structures. These approaches use many disparate technologies which include optics, magnetic and electrical fields, injection printing, physical or geometric constraints, or surface engineering (6-11). However, there is currently a paucity of a single method that can facilitate the formation of complex multicellular structures with high precision, high versatility, multiple dimensionality, and single-cell resolution, while maintaining cell viability, integrity, and function. As a result, there is a critical need to develop new methods that seek to overcome these limitations."Acoustic tweezers," which manipulate biological specimens using sound waves, offer several unique advantages (12, 13) in comparison with other techniques. First, the acoustic tweezers technology is the only active cell-manipulation method using gentle mechanical vibrations that do not alter cell characteristics. Acoustic vibrations create a pressure gradient in the medium to move suspended microobjects and cells, thereby resulting in a contaminationfree, contact-less, and label-free method for cell manipulation. Sound waves are preferred for cell manipulation for the following reasons: (i) cells maintain their native state (e.g., shape, size, reflective index,...

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Section: D Particle Manipulationmentioning

confidence: 99%

Three-dimensional manipulation of single cells using surface acoustic waves

Guo

Mao

Chen

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

461

331

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“…Despite significant advances in this field, to further understand how cells interact and communicate with each other, a robust, biocompatible method to precisely control the spatial and temporal association of cells and to create defined cellular assemblies is urgently needed (4). Although several methods have been used to pattern cells, limitations still exist for the demonstrated methods including those that make use of optical, electrical, magnetic, hydrodynamic, and contact printing technologies (5)(6)(7)(8)(9). Firstly, most of the methods require modification of the cell's native state.…”

mentioning

confidence: 99%

Controlling cell–cell interactions using surface acoustic waves

Guo

French

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

343

248

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The interactions between pairs of cells and within multicellular assemblies are critical to many biological processes such as intercellular communication, tissue and organ formation, immunological reactions, and cancer metastasis. The ability to precisely control the position of cells relative to one another and within larger cellular assemblies will enable the investigation and characterization of phenomena not currently accessible by conventional in vitro methods. We present a versatile surface acoustic wave technique that is capable of controlling the intercellular distance and spatial arrangement of cells with micrometer level resolution. This technique is, to our knowledge, among the first of its kind to marry high precision and high throughput into a single extremely versatile and wholly biocompatible technology. We demonstrated the capabilities of the system to precisely control intercellular distance, assemble cells with defined geometries, maintain cellular assemblies in suspension, and translate these suspended assemblies to adherent states, all in a contactless, biocompatible manner. As an example of the power of this system, this technology was used to quantitatively investigate the gap junctional intercellular communication in several homotypic and heterotypic populations by visualizing the transfer of fluorescent dye between cells.cell-cell interaction | intercellular communication | surface acoustic waves | acoustic tweezers | acoustofluidics M ulticellular systems rely on the interaction between cells to coordinate cell signaling and regulate cell functions. Understanding the mechanism and process of cell-cell interaction is critical to many physiological and pathological processes, such as embryogenesis, differentiation, cancer metastasis, immunological interactions, and diabetes (1-3). Despite significant advances in this field, to further understand how cells interact and communicate with each other, a robust, biocompatible method to precisely control the spatial and temporal association of cells and to create defined cellular assemblies is urgently needed (4). Although several methods have been used to pattern cells, limitations still exist for the demonstrated methods including those that make use of optical, electrical, magnetic, hydrodynamic, and contact printing technologies (5-9). Firstly, most of the methods require modification of the cell's native state. The magnetic assembly method, for example, requires cells to be labeled with magnetic probes. Dielectrophoresis typically requires the use of a special medium (e.g., nonconductive) which may lack essential nutrients or have biophysical properties (such as the osmolality) that may adversely affect cell growth or physiology (6). Optical tweezers provide a label-free and contactless approach, but typically require high laser power to manipulate cells, leading to a high risk of cell damage (5). Secondly, the working principles of the existing technologies mostly preclude the combination of high precision and high throughput into a single ...

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“…They are wellknown in medicine as MRI agents; in addition, their ability to respond to external magnetic fields gives an opportunity to develop cutting edge applications in protein and cell manipulation. 6 In particular, superparamagnetic iron oxide NPs (SPIONs) have attracted a lot of attention for drug delivery applications in theranostics (i.e., combined therapy and diagnosis). One of the promises of SPIONs is targeted delivery to the disease site following an external magnetic force.…”

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confidence: 99%