Magnetic immunochromatographic test for histamine detection in wine

Abstract

Histamine, a biogenic amine, is abundant in fermented foods and beverages, notably wine. A high intake of this monoamine may produce adverse reactions in humans, which may be severe in individuals with a reduced capacity to catabolize extrinsic histamine. Thus, control of histamine concentration during wine production and before distribution is advisable. Simple, rapid, point-of-use bioanalytical platforms are needed because traditional methods for the detection and quantification of histamine are expensive and time-consuming. This work applies the lateral flow immunoassay technique to histamine detection. Superparamagnetic particle labels, and an inductive sensor designed to read the test line in the immunoassay, enable magnetic quantification of the molecule. The system is calibrated with histamine standards in the interval of interest for wine production. A commercial optical strip reader is used for comparison measurements. The lateral flow system has a limit of detection of 1.2 and 1.5 mg/L for the inductive and optical readers, respectively. The capability of the inductive system for histamine quantification is demonstrated for wine samples at different processing points (at the end of alcoholic fermentation, at the end of malolactic fermentation, in freshly bottled wine, and in reserve wine). The results are validated by ultra-high-performance liquid chromatography.

The Performance of the Magneto-Impedance Effect for the Detection of Superparamagnetic Particles

“…The approach of using the impedance changes of nonmagnetic conductors to quantify the concentration of MNPs is being used successfully in the development of magnetic biosensors [28].…”

Recent Applications of Magnetic Nanoparticles in Food Analysis

“…Apart from the popular optical and electrochemical sensors, other interesting options based on m-NPs have been applied for food analysis. The main interest of these alternative sensors lies in the use of a different transduction principles (i.e., quartz crystal microbalance (QCM) [80,81], magnetic relaxation switching (MRS) [82][83][84], photoelectrochemistry [85,86]) or/and their particular arrangement (i.e., portable and low-cost sensors based on screen-printed electrode (ScPE) [73,85,[87][88][89][90][91][92], lateral flow strip (LFS) [93][94][95][96], microfluidic platforms [86,[97][98][99], and smartphone analysis [95,100,101]). Some selected examples of these kinds of devices are included in Table 6 [80,81,85,86,94,95,98,100,101].…”

“…This may be why not many LFS assays are commercially available. This kind of sensor has been employed together with m-NPs to analyse biogenic amines [93], oestrogenic compounds [94], globulin proteins [95] and botulinum neurotoxins [96] in beverages [93,96], milk [94,96], fish [94], meat [94] and legumes [95]. The main advantage of the introduction of m-NPs is the drastic increase in sensitivity and reduction of the LOD.…”

Ultrasensitive Magnetic Field Sensors for Biomedical Applications

“…In some cases, however, the traditional optical methods are not acceptable due to the high background noise or the low sensitivity of detecting devices. Because of these reasons, magnetic sensors have been employed [15][16][17].…”