Magnetic immobilization of a quorum sensing signal hydrolase, AiiA

Wang, Lin; Xu, Haixing; Liu, Zewen; Sun, Taolei; Yuan, Chengqing; Yang, Ying; Guo, Junhui; Xie, Hao

doi:10.1002/mbo3.797

Cited by 11 publications

(13 citation statements)

References 24 publications

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“…Additionally, they were able to enrich the MagR protein and MagR/Cry complex, respectively, from a complex matrix with a magnet and non-magnetized iron beads (Qin et al, 2016). Later, this method was also shown to be effective to capture MagR fusion proteins from a complex matrix (Jiang et al, 2017;Wang L. et al, 2019). However, the physical capabilities of this protein complex were recently questioned (Meister, 2016;Winklhofer and Mouritsen, 2016) and the putative magnetic properties have been still under review.…”

Section: Iron-sulfur Cluster Proteinsmentioning

confidence: 99%

Intrinsically Magnetic Cells: A Review on Their Natural Occurrence and Synthetic Generation

Pekarsky

Spadiut

2020

Front. Bioeng. Biotechnol.

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The magnetization of non-magnetic cells has great potential to aid various processes in medicine, but also in bioprocess engineering. Current approaches to magnetize cells with magnetic nanoparticles (MNPs) require cellular uptake or adsorption through in vitro manipulation of cells. A relatively new field of research is "magnetogenetics" which focuses on in vivo production and accumulation of magnetic material. Natural intrinsically magnetic cells (IMCs) produce intracellular, MNPs, and are called magnetotactic bacteria (MTB). In recent years, researchers have unraveled function and structure of numerous proteins from MTB. Furthermore, protein engineering studies on such MTB proteins and other potentially magnetic proteins, like ferritins, highlight that in vivo magnetization of non-magnetic hosts is a thriving field of research. This review summarizes current knowledge on recombinant IMC generation and highlights future steps that can be taken to succeed in transforming non-magnetic cells to IMCs.

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Section: Iron-sulfur Cluster Proteinsmentioning

confidence: 99%

Intrinsically Magnetic Cells: A Review on Their Natural Occurrence and Synthetic Generation

Pekarsky

Spadiut

2020

Front. Bioeng. Biotechnol.

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“…Qin et al also showed that the MagR protein and a MagR/Cryptochrome complex can be isolated and enriched from a complex matrix by silica-coated magnetite (SiO 2 -Fe 3 O 4 ) beads [5]. Later, MagR fusion proteins were successfully captured from a complex matrix [6,7].…”

Section: Introductionmentioning

confidence: 99%

“…Until now, the magnetic behavior of MagR has not been tested at low temperatures, which could give clearer indications on a potential magnetic behavior. Additionally, the Magnetochemistry 2021, 7, 147 2 of 8 stated usability of MagR fusion proteins for protein capture with magnetic beads [6,7] requires further characterization and comparison to state-of-the-art affinity downstream processing methods to reveal potential drawbacks or benefits. In this study, we deepened the investigation on MagR in two different aspects.…”

Section: Introductionmentioning

confidence: 99%

“…In this study, we deepened the investigation on MagR in two different aspects. First, we analyzed magnetic bead capture using recombinant MagR from the pigeon Columbia livia (clMagR) and MagR from Drosophila melanogaster (dMagR) [5][6][7]. Secondly, we tested if highly expressed MagR (>15% total intracellular soluble protein) would yield a magnetic moment in Escherichia coli cells at different temperatures to investigate if MagR expression would be sufficient to magnetize cells in vivo for diverse applications [13].…”

Section: Introductionmentioning

confidence: 99%

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Revisiting the Potential Functionality of the MagR Protein

2021

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Recent findings have sparked great interest in the putative magnetic receptor protein MagR. However, in vivo experiments have revealed no magnetic moment of MagR at room temperature. Nevertheless, the interaction of MagR and MagR fusion proteins with silica-coated magnetite beads have proven useful for protein purification. In this study, we recombinantly produced two different MagR proteins in Escherichia coli BL21(DE3) to (1) expand earlier protein purification studies, (2) test if MagR can magnetize whole E. coli cells once it is expressed to a high cytosolic, soluble titer, and (3) investigate the MagR-expressing E. coli cells’ magnetic properties at low temperatures. Our results show that MagR induces no measurable, permanent magnetic moment in cells at low temperatures, indicating no usability for cell magnetization. Furthermore, we show the limited usability for magnetic bead-based protein purification, thus closing the current knowledge gap between theoretical considerations and empirical data on the MagR protein.

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“…Magnetoreceptor (MagR) is a magnetic protein that can interact with external magnetic elds [14]. MagR was explored as a fusion tag with enzymes for functional immobilization of these enzymes on Fe 3 O 4 and Fe 3 O 4 @SiO 2 nanoparticles [15,16]. Magnetic interactions between MagR and supports reduced in uences of chemical environment and facilitated activities of enzymes over a broad range of environmental conditions.…”

Section: Introductionmentioning

confidence: 99%

Magnetic Immobilization of Dispersin B with Activity in Degradation of Bacterial Biofilm

Liu¹,

Zhao²,

Zeng³

et al. 2021

Preprint

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Dispersin B (DspB) is a member of glycoside hydrolase family 20 (GH20) and catalyzes degradation of biofilms forming by pathogenic bacteria such as Staphylococcus aureus. Magnetoreceptor (MagR) is a magnetic protein that can be used as a fusion partner for functionally immobilizing proteins on magnetic surfaces. In the present study, a recombinant protein DspB-MagR was constructed by fusing MagR to the C-terminus of DspB and expressed in Escherichia coli. Magnetic immobilization of purified DspB-MagR on Fe3O4@SiO2 nanoparticles was achieved and characterized by means of various techniques including SDS-PAGE, FTIR spectrometry, TGA measurements, zeta potential measurement, and SEM analysis. Stability and activity of immobilized DspB-MagR on Fe3O4@SiO2 nanoparticles were analyzed under different conditions such as temperature, pH, and storage time. Removal of biofilms forming by Staphylococcus aureus and other medical source bacterial species were achieved by using Fe3O4@SiO2 nanoparticles loading with DspB-MagR. This work promoted potential applications of DspB and similar enzymes for medical purposes.

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Magnetic immobilization of a quorum sensing signal hydrolase, AiiA

Cited by 11 publications

References 24 publications

Intrinsically Magnetic Cells: A Review on Their Natural Occurrence and Synthetic Generation

Intrinsically Magnetic Cells: A Review on Their Natural Occurrence and Synthetic Generation

Revisiting the Potential Functionality of the MagR Protein

Magnetic Immobilization of Dispersin B with Activity in Degradation of Bacterial Biofilm

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