Magnetic fluid hyperthermia enhances cytotoxicity of bortezomib in sensitive and resistant cancer cell lines

Alvarez-Berríos, Merlis P.; Castillo, Amalchi; Rinaldi, Carlos; Torres‐Lugo, Madeline

doi:10.2147/ijn.s51435

Cited by 26 publications

(11 citation statements)

References 38 publications

(45 reference statements)

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“…The same group reported similar extent of survival after WB and AMF at 43 and 45 °C at all treatment time tested (20,40,60,90,120 min) for semi-solids pellets of tumor, WiDr human colonic adenocarcinoma cells(1 × 10 8 −1 × 10 9 cells) 41 , 55 . On the other side, some works have reported a better efficiency in particle-induced cell death by magnetic hyperthermia when compared to WB protocols 56 . For example, Sanz et al .…”

Section: Discussionmentioning

confidence: 99%

Cell damage produced by magnetic fluid hyperthermia on microglial BV2 cells

Calatayud

Soler

Torres

et al. 2017

Sci Rep

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We present evidence on the effects of exogenous heating by water bath (WB) and magnetic hyperthermia (MHT) on a glial micro-tumor phantom. To this, magnetic nanoparticles (MNPs) of 30–40 nm were designed to obtain particle sizes for maximum heating efficiency. The specific power absorption (SPA) values (f = 560 kHz, H = 23.9 kA/m) for as prepared colloids (533–605 W/g) dropped to 98–279 W/g in culture medium. The analysis of the intracellular MNPs distribution showed vesicle-trapped MNPs agglomerates spread along the cytoplasm, as well as large (~0.5–0.9 μm) clusters attached to the cell membrane. Immediately after WB and MHT (T = 46 °C for 30 min) the cell viability was ≈70% and, after 4.5 h, decreased to 20–25%, demonstrating that metabolic processes are involved in cell killing. The analysis of the cell structures after MHT revealed a significant damage of the cell membrane that is correlated to the location of MNPs clusters, while local cell damage were less noticeable after WB without MNPs. In spite of the similar thermal effects of WB and MHT on the cell viability, our results suggest that there is an additional mechanism of cell damage related to the presence of MNPs at the intracellular space.

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Section: Discussionmentioning

confidence: 99%

Cell damage produced by magnetic fluid hyperthermia on microglial BV2 cells

Calatayud

Soler

Torres

et al. 2017

Sci Rep

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“…5 Some studies have reported enhanced cytotoxic effects of drugs such as cisplatin and bortezomib when used in combination with MFH, inducing sensitivity in resistant cancer cells. [6][7][8] In vivo studies have also demonstrated the thermal potentiation of drugs like cisplatin and doxorubicin via MFH, resulting in significant tumor volume reductions in mice. 9,10 Recently, Court et al investigated the thermal potentiation of 2-phenylethynesulfonamide (PES), an inhibitor of the function of the heat-shock proteins (HSP70).…”

Section: Introductionmentioning

confidence: 99%

<p>In vitro Ultrasonic Potentiation of 2-Phenylethynesulfonamide/Magnetic Fluid Hyperthermia Combination Treatments for Ovarian Cancer</p>

Mérida¹,

Rinaldi²,

Juan³

et al. 2020

IJN

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Background: Magnetic Fluid Hyperthermia (MFH) is a promising adjuvant for chemotherapy, potentiating the action of anticancer agents. However, drug delivery to cancer cells must be optimized to improve the overall therapeutic effect of drug/MFH combination treatments. Purpose: The aim of this work was to demonstrate the potentiation of 2-phenylethynesulfonamide (PES) at various combination treatments with MFH, using low-intensity ultrasound as an intracellular delivery enhancer. Methods: The effect of ultrasound (US), MFH, and PES was first evaluated individually and then as combination treatments. Definity ® microbubbles and polyethylene glycol (PEG)-coated iron oxide nanoparticles were used to induce cell sonoporation and MFH, respectively. Assessment of cell membrane permeabilization was evaluated via fluorescence microscopy, iron uptake by cells was quantified by UV-Vis spectroscopy, and cell viability was determined using automatic cell counting. Results: Notable reductions in cancer cell viability were observed when ultrasound was incorporated. For example, the treatment US+PES reduced cell viability by 37% compared to the non-toxic effect of the drug. Similarly, the treatment US+MFH using mild hyperthermia (41°C), reduced cell viability by an additional 18% when compared to the effect of MH alone. Significant improvements were observed for the combination of US+PES+MFH with cell viability reduced by an additional 26% compared to the PES+MFH group. The improved cytotoxicity was attributed to enhanced drug/nanoparticle intracellular delivery, with iron uptake values nearly twice those achieved without ultrasound. Various treatment schedules were examined, and all of them showed substantial cell death, indicating that the time elapsed between sonoporation and magnetic field exposure was not significant. Conclusion: Superior cancer cell-killing patterns took place when ultrasound was incorporated thus demonstrating the in vitro ultrasonic potentiation of PES and mild MFH. This work demonstrated that ultrasound is a promising non-invasive enhancer of PES/MFH combination treatments, aiming to establish a sono-thermo-chemotherapy in the treatment of ovarian cancer.

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“…Hence, the heating rate of the FePt–Au system is directly related with the magnetic character. Both the hydrophilic FePt and FePt–Au magnetic NPs could provide enough local heat (at least 43°C) <380 s to kill cancerous cells for potential cancer therapy treatments (Alvarez-Berríos et al, 2014 ; Chan et al, 2020 ; Espinosa et al, 2020 ). The cancer cells are destroyed at temperatures higher than 43°C, whereas the normal cells can be kept at higher temperatures.…”

Section: Resultsmentioning

confidence: 99%

Formation and Application of Core–Shell of FePt-Au Magnetic–Plasmonic Nanoparticles

et al. 2021

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Monodispersed FePt core and FePt–Au core–shell nanoparticles (NPs) have been chemically synthesized in liquid solution and with controllable surface-functional properties. The NP size was increased from 2.5 nm for FePt to 6.5 nm for FePt–Au, which could be tuned by the initial concentration of gold acetate coated onto FePt seeding NPs via a seed-mediated formation of self-assembled core–shell nanostructures. The analyses of the interplanar spacing obtained from the high-resolution transmission electron microscopy (HRTEM), selective electron diffraction pattern (SAED), and x-ray diffraction (XRD) confirmed that both FePt core and Au shell belong to the face-centered cubic (fcc) structure. FePt–Au NPs have a surface plasmon resonance (SPR) peak at 528 nm in the visible optical band region, indicating the red shift compared with the typical theoretical value of 520 nm of pure Au NPs. The surface modification and ligand exchange of FePt–Au was using mercaptoacetic acid (thiol) as a phase transfer reagent that turned the NPs hydrophilic due to the functional carboxyl group bond on the surface of presented multifunctional magnetic–plasmonic NPs. The water-dispersible FePt-based NPs conjugated with biomolecules could reach the different biocompatibility requirements and also provide enough heating response that acted as a potential agent for magnetic fluid hyperthermia in biomedical engineering research fields.

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Magnetic fluid hyperthermia enhances cytotoxicity of bortezomib in sensitive and resistant cancer cell lines

Cited by 26 publications

References 38 publications

Cell damage produced by magnetic fluid hyperthermia on microglial BV2 cells

Cell damage produced by magnetic fluid hyperthermia on microglial BV2 cells

<p>In vitro Ultrasonic Potentiation of 2-Phenylethynesulfonamide/Magnetic Fluid Hyperthermia Combination Treatments for Ovarian Cancer</p>

Formation and Application of Core–Shell of FePt-Au Magnetic–Plasmonic Nanoparticles

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