Cell damage produced by magnetic fluid hyperthermia on microglial BV2 cells

Calatayud, M. Pilar; Soler, E. Padron; Torres, Teobaldo E.; Campos‐González, E.; Junquera, Concepción; Ibarra, M. R.; Goya, Gerardo F.

doi:10.1038/s41598-017-09059-7

Cited by 49 publications

(50 citation statements)

References 68 publications

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“…Likewise, Wilhelm et al 12 obtained similar results for human prostatic tumor cells (PC3). Also, even Calatayud et al's 13 group mentioned above, in another comprehensive work, found no differences in viability of microglial BV2 cells subjected to hyperthermia induced by homogeneous water bath heating or a magnetically triggered one, contradicting apparent results obtained previously in other experimental conditions. 10 In this work, in order to shed some light on these seemingly inconsistent reports, we tested the comparative efficiency of WHT and MHT delivered by Fe-Cr-Nb-B magnetic nanoparticles with controllable Curie temperature 14 on commercial human osteosarcoma (hOS), MG-63 cell line (purchased from Sigma-Aldrich company, St Louis, MO, USA).…”

Section: Introductionmentioning

confidence: 68%

Comparative effects of magnetic and water-based hyperthermia treatments on human osteosarcoma cells

Herea¹,

Danceanu²,

Radu³

et al. 2018

IJN

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IntroductionHyperthermia (HT) based on magnetic nanoparticles (MNPs) represents a promising approach to induce the apoptosis/necrosis of tumor cells through the heat generated by MNPs submitted to alternating magnetic fields. However, the effects of temperature distribution on the cancer cells’ viability as well as heat resistance of various tumor cell types warrant further investigation.MethodsIn this work, the effects induced by magnetic hyperthermia (MHT) and conventional water-based hyperthermia (WHT) on the viability of human osteosarcoma cells at different temperatures (37°C–47°C) was comparatively investigated. Fe-Cr-Nb-B magnetic nanoparticles were submitted either to alternating magnetic fields or to infrared radiation generated by a water-heated incubator.ResultsIn terms of cell viability, significant differences could be observed after applying the two HT treatment methods. At about equal equilibrium temperatures, MHT was on average 16% more efficient in inducing cytotoxicity effects compared to WHT, as assessed by MTT cytotoxicity assay.ConclusionWe propose the phenomena can be explained by the significantly higher cytotoxic effects initiated during MHT treatment in the vicinity of the heat-generating MNPs compared to the effects triggered by the homogeneously distributed temperature during WHT. These in vitro results confirm other previous findings regarding the superior efficiency of MHT over WHT and explain the cytotoxicity differences observed between the two antitumor HT methods.

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Section: Introductionmentioning

confidence: 68%

Comparative effects of magnetic and water-based hyperthermia treatments on human osteosarcoma cells

Herea¹,

Danceanu²,

Radu³

et al. 2018

IJN

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“…[21][22][23] Currently, most of the in vitro studies that analyse the efficacy of the hyperthermia treatment are being performed in monolayer cell cultures. [24][25][26] However, this traditional cell culture method, in two dimensions (2D), has essential limitations in terms of inter-cellular communication, cell microenvironment and cell spatial behaviour. 27 Therefore, these 2D cell culture models cannot replicate the morphology and biochemical properties the cells have in the living organisms.…”

Section: Introductionmentioning

confidence: 99%

Dual Role of Magnetic Nanoparticles as Intracellular Hotspots and Extracellular Matrix Disruptors Triggered by Magnetic Hyperthermia in 3D Cell Culture Models

Beola

Asín

Fratila

et al. 2018

ACS Appl. Mater. Interfaces

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Magnetic hyperthermia is a promising therapy for the localized treatment of cancer based on the exposure of magnetic nanoparticles to an external alternating magnetic field. In order to evaluate some of the mechanisms involved in the cellular damage caused by this treatment, two different 3D cell culture models were prepared using collagen, which is the most abundant protein of the extracellular matrix. The same amount of nanoparticles was added to cells either before or after their incorporation to the 3D structure. Therefore, in one model, particles were located only inside cells (In model), while the other one had particles both inside and outside cells (In&Out model). In the In&Out model, the hyperthermia treatment facilitated the migration of the particles from the outer areas of the 3D structure to the inner parts, achieving a faster homogeneous distribution throughout the whole structure and allowing the particles to gain access to the inner cells. The cell death mechanism activated by the magnetic hyperthermia treatment was different in both models. Necrosis was observed in the In model while apoptosis in the In&Out model 24 hours after the hyperthermia application. This was clearly correlated with the amount of nanoparticles located inside the cells. Thus, the combination of both 3D models allowed us to

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“…In order to achieve maximum therapeutic effects, the function of antigenicity, adjuvanticity, and feedback inhibition, three important factors in reactivating an antitumor immune response, should be comprehensively coordinated when designing efficient collaborative strategies . Tailoring biomaterials to integrate multiple other therapies such as gene therapy, cell therapy, magnetic fluid hyperthermia, and so on will provide great promise in the development of ICB therapy. Considering the relatively low response rate associated with ICB, identification of predictive and pharmacodynamic biomarkers will be crucial to guide the choice of inhibitor .…”

Section: Resultsmentioning

confidence: 99%

Toward Biomaterials for Enhancing Immune Checkpoint Blockade Therapy

Fan

Chen

Wang

et al. 2018

Adv Funct Materials

105

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Despite the remarkable progress in immune checkpoint blockade (ICB) therapy for cancer treatment, low objective response and immune‐related side effects (immune‐related adverse events, irAEs) limit the further development of ICBs. To address these challenges and enhance the efficiency of cancer immunotherapy, the emerging interest has focused on manipulating biomaterials to form innovational drug delivery systems that are necessary to effectively deliver immune checkpoint inhibitors. Such biomaterial‐based strategies can improve accumulation, control release, and enhance retention of checkpoint inhibitors within target locations while simultaneously reducing drug exposure for off‐target tissues, thereby optimizing both the efficacy and safety. In addition, with the assistance of biomaterials, combinations of ICB and conventional treatment strategies including chemotherapy, radiotherapy, and phototherapy are designed to further enhance the response rate of ICB. This review focuses on the latest reports on engineering biomaterials to improve the antitumor efficiency of ICB, with stress on antibody‐, gene‐, and trap protein–based immune checkpoint blockade strategies and their combinations with conventional therapies. Challenges and future trends in engineering biomaterials to modulate immune checkpoint therapy are discussed.

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Cell damage produced by magnetic fluid hyperthermia on microglial BV2 cells

Cited by 49 publications

References 68 publications

Comparative effects of magnetic and water-based hyperthermia treatments on human osteosarcoma cells

Comparative effects of magnetic and water-based hyperthermia treatments on human osteosarcoma cells

Dual Role of Magnetic Nanoparticles as Intracellular Hotspots and Extracellular Matrix Disruptors Triggered by Magnetic Hyperthermia in 3D Cell Culture Models

Toward Biomaterials for Enhancing Immune Checkpoint Blockade Therapy

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