Magnetic field-induced changes in specific gene transcription

Phillips, J. E.; Haggren, Wendy; Thomas, William J.; Ishida-Jones, Tamako; Adey, W. R.

doi:10.1016/0167-4781(92)90004-j

Cited by 166 publications

(69 citation statements)

References 16 publications

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“…Using high-throughput analysis, we found that genome-wide expression levels in MCF10A human breast epithelial cells were not changed by exposure to ELF-MFs. To confirm these results, we performed sqRT-PCR on six genes selected from the microarray analysis that exhibited statistically non-significant trends for expression changes; the follow-up results confirmed those of the Previous studies reported that ELF-MF exposure could affect transcription levels in vitro of several immediate-response genes [8], [19]～ [21]. These studies, however, only looked at a few genes and their results require further confirmation before firm conclusions can be drawn.…”

Section: ⅳ Discussionsupporting

confidence: 56%

Effect of Extremely Low Frequency Magnetic Fields on Gene Expression in Human Mammary Epithelial MCF10A Cells

Hong¹,

Lee²,

Kim³

et al. 2012

Journal of electromagnetic engineering and science

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The effects of extremely low frequency magnetic fields (ELF-MFs) on physiological processes at the cellular level remain unclear despite a number of studies. To investigate the effects of ELF-MFs on gene expression, we exposed human mammary epithelial MCF10A cells to fields of 1 mT magnetic flux density at 60 Hz for 4 and 16 h and measured the transcriptional responses of 24,000 genes using Illumina microarrays. In three independent experiments, we found no statistically significant alteration of expression levels for any of the genes assayed using a cutoff value of 1.2-fold. To confirm this result, we selected six genes with trends suggesting possible expression level changes, although these trends were not statistically significant, and investigated their expression levels further using a semiquantitative reverse-transcription polymerase chain reaction. In three independent experiments, we did not find any alterations in the expression levels of these genes. From these results, we conclude that ELF-MFs do not affect gene expression profiles under our exposure conditions.

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Section: ⅳ Discussionsupporting

confidence: 56%

Effect of Extremely Low Frequency Magnetic Fields on Gene Expression in Human Mammary Epithelial MCF10A Cells

Hong¹,

Lee²,

Kim³

et al. 2012

Journal of electromagnetic engineering and science

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“…In yet another manifestation, as we note below, EM field induction of the stress protein hsp70 may provide a useful biomarker for establishing a science-based safety standard for the design of cell phones and their transmission towers. cell calcium levels (Liburdy, 1992;Liburdy et al, 1993) and increased transcript levels for the immediate early response genes myc (Lin et al, 1994(Lin et al, , 1996Jin et al, 1997), jun, and fos (Phillips et al, 1992;Rao and Henderson, 1996) and the stress response gene HSP70 . In cell-free systems, increases in enzyme reaction rates include ornithine decarboxylase (Byus et al, 1988), Na,K-ATPase, and cytochrome oxidase (Blank, 1995b;Blank and Soo, 1998).…”

mentioning

confidence: 99%

Insights into electromagnetic interaction mechanisms

Goodman

Blank

2002

Journal Cellular Physiology

166

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Low frequency (< 300 Hz) electromagnetic (EM) fields induce biological changes that include effects ranging from increased enzyme reaction rates to increased transcript levels for specific genes. The induction of stress gene HSP70 expression by exposure to EM fields provides insight into how EM fields interact with cells and tissues. Insights into the mechanism(s) are also provided by examination of the interaction of EM fields with moving charges and their influence on enzyme reaction rates in cell-free systems. Biological studies with in vitro model systems have focused, in general, on the nature of the signal transduction pathways involved in response to EM fields. It is likely, however, that EM fields also interact directly with electrons in DNA to stimulate biosynthesis. Identification of an EM field-sensitive DNA sequence in the heat shock 70 (HSP70) promoter, points to the application of EM fields in two biomedical applications: cytoprotection and gene therapy. EM field induction of the stress protein hsp70 may also provide a useful biomarker for establishing a science-based safety standard for the design of cell phones and their transmission towers.

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“…Currently, there are abundant evidences that various types of EFs, MFs and EMFs affect functional processes in microorganisms and influence their pathogenicity potential. It was also proved that EFs, MFs and EMFs can influence the biological functions of different organisms which affect hormone secretion [5], enzyme activity [6], cell viability [7], cell proliferation [7,8], transport of ions [9], cell metabolic activity [10] or synthesis and transcription of DNA [11]. The MFs and EMFs were also used in medicines as modulators of bacteria-host interaction during trials of developing alternative therapies [4,12], and as potential agents to modulate the properties of microorganisms involved in biotechnological processes [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

The Effects of Rotating Magnetic Field on Growth Rate, Cell Metabolic Activity and Biofilm Formation by Staphylococcus Aureus and Escherichia Coli

kowski¹,

Nawrotek²,

Struk³

et al. 2013

Journal of Magnetics

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This work presents results of the study which concerns the influence of the rotating magnetic field (RMF) on the growth rate, cell metabolic activity and ability to form biofilms by E. coli and S. aureus. Liquid cultures of the bacteria were exposed to the RMF (RMF frequency f = 1-50 Hz, RMF magnetic induction B = 22-34 mT, time of exposure t = 60 min, temperature of incubation 37 °C). The present study indicate the exposition to the RMF, as compared to the unexposed controls causing an increase in the growth dynamics, cell metabolic activities and percentage of biofilm-forming bacteria, in both S. aureus and E. coli cultures. It was also found that the stimulating effects of the RMF exposition enhanced with its increasing frequencies and magnetic inductions.

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Magnetic field-induced changes in specific gene transcription

Cited by 166 publications

References 16 publications

Effect of Extremely Low Frequency Magnetic Fields on Gene Expression in Human Mammary Epithelial MCF10A Cells

Effect of Extremely Low Frequency Magnetic Fields on Gene Expression in Human Mammary Epithelial MCF10A Cells

Insights into electromagnetic interaction mechanisms

The Effects of Rotating Magnetic Field on Growth Rate, Cell Metabolic Activity and Biofilm Formation by Staphylococcus Aureus and Escherichia Coli

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