Magnetic Cell–Scaffold Interface Constructed by Superparamagnetic IONP Enhanced Osteogenesis of Adipose-Derived Stem Cells

Chen, Huimin; Sun, Jianfei; Wang, Zibin; Zhou, Yi; Lou, Zhichao; Chen, Bin; Wang, Peng; Guo, Zhirui; Tang, Hui; Ma, Junqing; Xia, Yang; Gu, Ning; Zhang, Feimin

doi:10.1021/acsami.8b17427

Cited by 71 publications

(55 citation statements)

References 57 publications

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“…In order to confirm the role of the magnetic properties of the NPs, Chen et al. have prepared composite PLGA‐PCL fibrous scaffold with two types of NPs at their surface, by post‐electrospinning layer‐by‐layer coatings of IO NPs or gold NPs . Both NP coatings increased the hydrophilicity of the fibers and the elastic modulus of the scaffolds, as well as its protein adsorption ability, which resulted in a better adhesion of adipose‐derived stem cells (ADSC).…”

Section: Applications In Tissue Engineeringmentioning

confidence: 99%

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Nanocomposite Polymer Scaffolds Responding under External Stimuli for Drug Delivery and Tissue Engineering Applications

Mertz

Harlepp

Goetz

et al. 2019

Advanced Therapeutics

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The blossoming development of nanomaterials and polymer science has opened the way toward new biocompatible scaffolds responding remotely to external stimuli (magnetic field, light, electric field). Such smart scaffolds are envisioned as new implantable tissues displaying multiple therapeutic and imaging functionalities. They hold great promises to achieve a controlled delivery of therapeutics for various diseases, or to ensure a stimuli‐induced cellular response for bone, cardiac, or muscle tissue engineering.

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Section: Applications In Tissue Engineeringmentioning

confidence: 99%

“…ISCA1, iron‐sulfur cluster assembly protein 1; ALP, alkaline phosphatase; COL1, collagen type 1; RUNX, runt‐related transcription factor; OCN, osteocalcin. Reproduced with permission . Copyright 2018, American Chemical Society.…”

Section: Applications In Tissue Engineeringmentioning

confidence: 99%

Nanocomposite Polymer Scaffolds Responding under External Stimuli for Drug Delivery and Tissue Engineering Applications

Mertz

Harlepp

Goetz

et al. 2019

Advanced Therapeutics

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show abstract

“…IO-NPs were also incorporated into polymeric scaffolds which were fabricated via electrospinning [133], or via composite fabrication [134] and 3D printing [135,136]. Biocompatible polymers explored so far include PLA [137], poly(lactic-co-glycolic acid) (PCL) [138], poly(urethane) [139], and chitosan [134]. A drawback for polymer-based scaffolds is their low mechanical strength.…”

Section: Bone Tissue Engineeringmentioning

confidence: 99%

Cerium- and Iron-Oxide-Based Nanozymes in Tissue Engineering and Regenerative Medicine

Jansman

Hosta‐Rigau

2019

Catalysts

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Nanoparticulate materials displaying enzyme-like properties, so-called nanozymes, are explored as substitutes for natural enzymes in several industrial, energy-related, and biomedical applications. Outstanding high stability, enhanced catalytic activities, low cost, and availability at industrial scale are some of the fascinating features of nanozymes. Furthermore, nanozymes can also be equipped with the unique attributes of nanomaterials such as magnetic or optical properties. Due to the impressive development of nanozymes during the last decade, their potential in the context of tissue engineering and regenerative medicine also started to be explored. To highlight the progress, in this review, we discuss the two most representative nanozymes, namely, cerium- and iron-oxide nanomaterials, since they are the most widely studied. Special focus is placed on their applications ranging from cardioprotection to therapeutic angiogenesis, bone tissue engineering, and wound healing. Finally, current challenges and future directions are discussed.

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“…Effects on the cell activities: The effect of GNPs on cell differentiation and maturation has been highlighted in another study. It has been observed that the cells developed longer neuronal outgrowth in the presence of GNPs [57,58]. Other effects: The exposure of the cell to NPs brings about harmful effects such as damage mitochondrial function, inflammation, the formation of apoptotic bodies, membrane leakage of lactate dehydrogenase, reactive oxygen species (ROS) production, increase in micronuclei, and chromosome condensation [49].…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Multidisciplinary Approach toward High Throughput Label-Free Cytotoxicity Monitoring of Superparamagnetic Iron Oxide Nanoparticles

2019

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This paper focuses on cytotoxicity examination of superparamagnetic iron oxide nanoparticles (SPIONs) using different methods, including impedance spectroscopy. Recent advances of SPIONs for clinical and research applications have triggered the need to understand their effects in cells. Despite the great advances in adapting various biological and chemical methods to assess in-vitro toxicity of SPIONs, less attention has been paid on the development of a high throughput label-free screening platform to study the interaction between the cells and nanoparticles including SPIONs. In this paper, we have taken the first step toward this goal by proposing a label-free impedimetric method for monitoring living cells treated with SPIONs. We demonstrate the effect of SPIONs on the adhesion, growth, proliferation, and viability of neuroblastoma 2A (N2a) cells using impedance spectroscopy as a label-free method, along with other standard microscopic and cell viability testing methods as control methods. Our results have shown a decreased viability of the cells as the concentration of SPIONs increases with percentages of 59%, 47%, and 40% for 100 µg/mL (C4), 200 µg/mL (C5), 300 µg/mL (C6), respectively. Although all SPIONs concentrations have allowed the growth of cells within 72 h, C4, C5, and C6 showed slower growth compared to the control (C1). The growth and proliferation of N2a cells are faster in the absence or low concentration of SPIONS. The percent coefficient of variation (% CV) was used to compare cell concentrations obtained by TBDE assay and a Scepter cell counter. Results also showed that the lower the SPIONs concentration, the lower the impedance is expected to be in the sensing electrodes without the cells. Meanwhile, the variation of surface area (∆S) was affected by the concentration of SPIONs. It was observed that the double layer capacitance was almost constant because of the higher attachment of cells, the lower surface area coated by SPIONs. In conclusion, impedance changes of electrodes exposed to the mixture of cells and SPIONs offer a wide dynamic range (>1 MΩ using Electric Cell-substrate Impedance electrodes) suitable for cytotoxicity studies. Based on impedance based, viability testing and microscopic methods’ results, SPIONs concentrations higher than 100 ug/mL and 300 ug/mL cause minor and major effects, respectively. We propose that a high throughput impedance-based label-free platform provides great advantages for studying SPIONs in a cell-based context, opening a window of opportunity to design and test the next generation of SPIONs with reduced toxicity for biomedical or medical applications.

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Magnetic Cell–Scaffold Interface Constructed by Superparamagnetic IONP Enhanced Osteogenesis of Adipose-Derived Stem Cells

Cited by 71 publications

References 57 publications

Nanocomposite Polymer Scaffolds Responding under External Stimuli for Drug Delivery and Tissue Engineering Applications

Nanocomposite Polymer Scaffolds Responding under External Stimuli for Drug Delivery and Tissue Engineering Applications

Cerium- and Iron-Oxide-Based Nanozymes in Tissue Engineering and Regenerative Medicine

A Multidisciplinary Approach toward High Throughput Label-Free Cytotoxicity Monitoring of Superparamagnetic Iron Oxide Nanoparticles

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