Magnetic beads based rolling circle amplification–electrochemiluminescence assay for highly sensitive detection of point mutation

Su, Qiang; Xing, Da; Zhou, Xiaoming

doi:10.1016/j.bios.2009.11.025

Cited by 72 publications

(17 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, if excessive beads were used, the electrode would be over-saturated by the adsorbed beads. As a result, the reaction of TPrA and Ru(bpy) 3 2+ on the electrode surface could be blocked, resulting in a decreased ECL (Tang et al 2007;Su et al 2010). It was therefore necessary to find the optimum quantity of beads for this detection system, and the tests showed that 4 μg of the beads was the appropriate amount to detect a 20-μL hybridization mixture of P. minimum in the proposed method.…”

Section: Discussionmentioning

confidence: 96%

“…It not only allowed rapid and efficient separation, but also served as a high throughput platform for detection. Magnetic beads have been proven valuable in immune and nucleic acid assays because of their improved sensitivity and selectivity (Palecek and Fojta 2007;Yang et al 2008;Zhu et al 2010;Su et al 2010;Horák et al 2011). The quantity of magnetic beads was also of vital importance for ECL detection.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Detection of Prorocentrum minimum (Pavillard) Schiller with an Electrochemiluminescence–Molecular Probe Assay

Zhu

Zhen

et al. 2012

Mar Biotechnol

View full text Add to dashboard Cite

The occurrence of harmful algal blooms (HABs) caused by Prorocentrum minimum (Pavillard) Schiller is a crucial subject in the study of HABs. An electrochemiluminescence-molecular probe assay (ECL-MP) was developed to qualitatively and quantitatively detect P. minimum. It was based on the sandwich hybridization integrated with a nuclease protection assay (NPA-SH) and improved by electrochemiluminescence (ECL). An ECL analyzer was established in this study, and it was shown that this analyzer was stable and highly sensitive, with a detection range of 0.4 pmol to 4 nmol Ru(bpy)(3)Cl(2)·6H(2)O under optimal reaction conditions of 1.0 V, 1.0 mA, 1.5 mol·L(-1) TPrA, and pH 7.4. The optimal amount of magnetic beads for separation of labeled NPA probes in a 20-μL hybridization mixture was 4 μg. The ECL counts per second was linear with the number of P. minimum cells in a range of 6.25 × 10(2) to 4 × 10(4), and there was no significant difference between ECL-MP and microscopy, with a 95% confidence level (t test) when individual, mixed cultures and field samples were treated. This study provides a convenient method for fast and accurate detection of P. minimum in the marine environment.

show abstract

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Detection of Prorocentrum minimum (Pavillard) Schiller with an Electrochemiluminescence–Molecular Probe Assay

Zhu

Zhen

et al. 2012

Mar Biotechnol

View full text Add to dashboard Cite

show abstract

“…To date, various sensors have been developed with RCA based on electrochemistry [45–47], optics [48–50], fluorescence [51–53], chemiluminescence [54,55], and other assays [56]. Schopf et al presented a bead-based RCA in a sandwich assay to detect viral DNA by hybridizing padlock probes with 1 μm diameter beads as surfaces for RCA [57].…”

Section: Isothermal Nucleic Acid Amplificationmentioning

confidence: 99%

Diagnostic Devices for Isothermal Nucleic Acid Amplification

Chang

Chen

Wei

et al. 2012

Sensors

125

View full text Add to dashboard Cite

Since the development of the polymerase chain reaction (PCR) technique, genomic information has been retrievable from lesser amounts of DNA than previously possible. PCR-based amplifications require high-precision instruments to perform temperature cycling reactions; further, they are cumbersome for routine clinical use. However, the use of isothermal approaches can eliminate many complications associated with thermocycling. The application of diagnostic devices for isothermal DNA amplification has recently been studied extensively. In this paper, we describe the basic concepts of several isothermal amplification approaches and review recent progress in diagnostic device development.

show abstract

“…DNA detection is of great importance in clinical testing, pathogen detection, forensic chemistry and mutated gene diagnosis associated with human diseases. Owing to its excellent analytical performance, Ru(bpy) 3 2+ ‐based ECL has been widely used in DNA detection .…”

Section: Ru(bpy)32+‐based Ecl For Bioapplicationsmentioning

confidence: 99%

Electrochemiluminescence of tris(2,2′‐bipyridyl)ruthenium and its applications in bioanalysis: a review

2011

View full text Add to dashboard Cite

Electrochemiluminescence (ECL) of tris(2,2'-bipyridyl)ruthenium(II) [Ru(bpy)(3) (2+)] is an active research area and includes the synthesis of ECL-active materials, mechanistic studies and broad applications. Extensive research has been focused on this area, due to its scientific and practical importance. In this mini-review we focus on the bio-related applications of ECL. After a brief introduction to Ru(bpy)(3) (2+) ECL and its mechanisms, its application in constructing an effective bioassay is discussed in detail. Three types of ECL assay are covered: DNA, immunoassay and functional nucleic acid sensors. Finally, future directions for these assays are discussed.

show abstract

Magnetic beads based rolling circle amplification–electrochemiluminescence assay for highly sensitive detection of point mutation

Cited by 72 publications

References 46 publications

Detection of Prorocentrum minimum (Pavillard) Schiller with an Electrochemiluminescence–Molecular Probe Assay

Detection of Prorocentrum minimum (Pavillard) Schiller with an Electrochemiluminescence–Molecular Probe Assay

Diagnostic Devices for Isothermal Nucleic Acid Amplification

Electrochemiluminescence of tris(2,2′‐bipyridyl)ruthenium and its applications in bioanalysis: a review

Contact Info

Product

Resources

About