Magnetic bead-gold nanoparticle hybrids probe based on optically countable gold nanoparticles with dark-field microscope for T4 polynucleotide kinase activity assay

An algorithm-assisted automated identification and enumeration system for sensitive hydrogen sulfide sensing under dark field microscopy

“…Then, excess sulfuric acid was added to the tube. Owing to the low pH value, almost all sulfides form H 2 S molecules rather than HS − or S. 2–49 The low solubility made the H 2 S molecule volatilize and come into contact with AuNPs-N 3 -Alk and further reduced the azido group to an amino group, which has been proven by many works. 50–53 Therefore, the cuprous-catalysed click chemistry reaction was blocked, resulting in single AuNPs in the solution.…”

“…Thus, the scattering colour of AuNPs changes under DFM, and this has been regarded as the basis for target analysis. 29–32 These methods were developed based on a general image processing software ( e.g. , ImageJ) that collects the scattering signal changes and quickly corresponds to the target concentration.…”

“…Thus, the scattering colour of AuNPs changes under DFM, and this has been regarded as the basis for target analysis. [29][30][31][32] These methods were developed based on a general image processing software (e.g., ImageJ) that collects the scattering signal changes and quickly corresponds to the target concentration. Our group also performed research by using Image J software as a data processor [33][34][35] for poisonous substance and biomolecule detection.…”

An Exonuclease I-Aided Turn-Off Fluorescent Strategy for Alkaline Phosphatase Assay Based on Terminal Protection and Copper Nanoparticles

“…Due to the simplicity, high sensitivity, low cost, and rapidness, nanomaterial-based probes have attracted considerable attention lately [ 26 ]. On account of the fluorescence of some nanoparticles, including copper nanoparticles (CuNPs), silver nanoparticles (AgNPs), and gold nanoparticles (AuNPs), the signals can be monitored by spectrometers [ 27 , 28 ]. In recent years, some efforts have been made towards the detection of ALP by measuring the fluorescence intensity of nanoparticles.…”

Electrochemical DNA Sensors Based on MoS2-AuNPs for Polynucleotide Kinase Activity and Inhibition Assay

“…The traditional detection strategies for PNK activity including polyacrylamide gel electrophoresis (PAGE), radical isotope P-labeling, and autoradiography have been commonly used in biological experiments due to their accurate quantification. − However, the applications of these methods are limited by their laborious, complicated, and radioactive operations. To overcome these drawbacks, many approaches such as fluorescence, − colorimetric, bioluminescence, and electrochemical assays − have been developed. Among them, electrochemical assays have attracted increasing attention due to their obvious advantages of low cost, easy miniaturization, high sensitivity, and fast response. − Zhang et al constructed an electrochemical biosensor for the determination of PNK with a detection limit of 0.0027 U/mL by phos-tag-biotin-mediated alkaline phosphatase catalytic amplification .…”