Magnetic bead-based separation of sperm cells from semen-vaginal fluid mixed stains using an anti-ACRBP antibody

Li, Xiaona; Xu, Feng‐ling; Zheng, Jianyi; Sun, Mao-Ling; Zhu, Xiaojun; Lv, Peng; Du, Zhe; Zhang, Xiu-peng; Yao, Jun

doi:10.1007/s00414-022-02917-8

Cited by 2 publications

(1 citation statement)

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“…The process followed the methodology outlined in our previous study [17]. To label the ABO blood group antibody with biotin, 50 µL of ABO blood group antibody (Abcam) was added to a 2 mL reaction tube equipped with magnetic stirring columns.…”

Section: Methodsmentioning

confidence: 99%

Separation mixed semen of two individuals using magnetic beads coupled ABH blood group antibody

Yao,

Adnan,

Wang

2023

Electrophoresis

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In sexual assault cases, one of the most common samples collected is a mixed semen stain, which is often found on the vagina, female underwear, or bed sheets. However, it is usually difficult to identify the perpetrator based on this sample alone. One technique that has been developed to address this issue is magnetic bead‐based separation. This method involves using modified magnetic microspheres to capture and enrich specific target cells, in this case, sperm cells. In this study, we utilized magnetic beads coupled with ABH blood group antibody to isolate sperm cells from an individual of a single ABO blood type. Subsequently, polymerase chain reaction amplification and capillary electrophoresis were employed to perform the genotyping the short tandem repeat (STR) loci. This approach allows for the identification of different individuals in a mixed seminal stain sample from two individuals, by first separating sperm cells based on ABH antigen differences and subsequently utilizing autosomal STR typing on the enriched single blood group cells.

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Section: Methodsmentioning

confidence: 99%