The polyamines putrescine, spermidine, and spermine prevent the loss of chlorophyl normaDly associated with senescence of excised leaf tissue maintained in darkness on water (control). Retention of chlorophyl in barley leaf discs was in the range of 90% 4 days after excision and placement on effective polyamine solutions. In contrast, the loss of soluble protein was hastened with 0.5 miLimolar spermidine and spermine treatments but it was retarded by 0.5 millimolar putrescine.Photosystem I and II activities of chloroplasts from polyamine-treated leaf discs declined more rapidly as compared to the control. Chloroplast ultrastructural changes resulting from the polyamine treatments included the apparent destruction of the envelope, preservation of thylakoid membrane structure, and reduced accumulation of osmiophilic bodies. The influence of polyamines on senescence-related processes may be due to their cationic nature.Natural and artificially induced senescence of leaf tissue have been used to study the effects of a number of metabolites and growth regulators on protein metabolism and on the structure and photochemical properties of chloroplasts (9,16,18,23,26,32,36). In this respect it has been found that kinetin can retard the characteristic loss of protein and pigments during leaf senescence (7,15,22) as well as preserve chloroplast structure (7) and the integrity of the electron transport chain (15). Poovaiah and Leopold (27) have reported that inorganic cations, particularly Ca, can also defer the symptoms of leaf senescence, an effect they attributed to the maintenance of membrane integrity.The stabilization of membrane integrity by Ca and the naturally occurring organic cations, the polyamines, has been shown with osmotically sensitive forms of different bacteria (19,30). More recently, polyamines have also been found to be capable of retarding the progressive senescence of protoplasts isolated from oat leaves (1, 17). There are many examples from both prokaryotes and eukaryotes of polyamine involvement in the regulation of macromolecular synthesis (6, 10, 13, 31), and it has been shown that polyamines can replace the Mg2" requirement for protein synthesis in a barley in vitro system (12). The present study was undertaken to determine the effects of polyamines on Chl and protein retention, photochemical activity, and chloroplast ultrastructure of barley leaf discs during senescence.