Seedlings of two barley genotypes ('Maresi' and wild form ofHordeum spontaneum) were treated with jasmonic acid (JA 5 laM and 15 laM) for 24 h, and then subjected to water stress (PEG 6000 solution of-1.5 MPa). JA caused an increase in the content of ABA but not in that of proline and spermidine in the two studied genotypes. The effect of the treatment did not depend on the applied JA concentration. The pre-stress treatment with JA changed plant response to water deficit with regard to membrane injury. Treatment with a lower JA concentration (5 laM) caused a substantial reduction of the stress-induced membrane damage in the both genotypes. A higher JA concentration (15 laM) caused the reduction of membrane injury only in H. spontaneum and was ineffective in 'Maresi'. JA had no influence on the leaf water status in water-stressed plants. A possible role of JA in leaf ABA accumulation and alleviation of cell membrane injury under water deficit is discussed.
Short-term treatment of potato (Solanum tuberosum L.) tuber discs with CdCl 2 solution elevated both the StPCS1 transcript level, phytochelatin synthase (PCS) activity and contents of phytochelatin (PC) and abscisic acid (ABA). Similar effects but less marked were noticed after treatment of tuber tissues with ABA solution. Cd-treatment increased also cysteine (CYS) content but did not change glutathione content. If ABA treatment preceded Cd-treatment, the elevation of CYS and PC contents were limited. The data suggest the participation of ABA in the regulation of PCS.
Treatment of potato (Solanum tuberosum) plants with cadmium or abscisic acid (ABA) enhanced the content of StPCS1 transcript and activity of phytochelatin synthase (PCS) in roots. These treatments enhanced the contents of ABA and expression of genes coding 9-cis-epoxycarotenoid dioxygenase 1 (NCED1) and basic leucine zipper (b-ZIP). Simultaneous treatment of potato plants with Cd and fluridone (Flu), an inhibitor of ABA biosynthesis, completely halted the Cd-induced transcription of StPCS1, NCED1, and StbZIP genes and limited the increases in PCS activity and ABA content. The data suggest that ABA participates in transduction of the Cd signal to the cells of potato roots.
The purpose of the study was to examine water stress-induced changes in the ABA and proline contents in roots and leaves of a potentially more resistant wild accession ofHordeum spontaneum and the modem cultivar Maresi (Hordeum vulgare). Leaves of H. spontaneum had higher contents of constitutive ABA and proline in comparison to those of 'Maresi'. A moderate water deficit resulted only in root dehydration, which was higher in 'Maresi'. Increases of water deficit in roots coincided with an increase of ABA content in roots, followed by that in leaves. The level of proline increased only in leaves and only in the case of H. spontaneum. Under conditions of severe water stress, the root dehydration levels were similar in the both genotypes, whereas leaf dehydration was higher in 'Maresi'. H. spontaneum, as compared to 'Maresi' showed an earlier increase of ABA content in the roots and accumulated more ABA in the leavesl Free proline levels in the roots increased in both genotypes but H. spontaneurn exhibited a 2-fold higher proline accumulation than 'Maresi'. In 1t. spontaneum the accumulation of proline in the leaves occurred noticeably earlier and to a higher extent than in 'Maresi'. A possible connection of these modifications with water stress resistance of the investigated genotypes is discussed in this paper.
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