Magic wands of CRISPR—lots of choices for gene knock-in

Sakuma, Tetsushi; Yamamoto, Takashi

doi:10.1007/s10565-017-9409-6

Cited by 25 publications

(8 citation statements)

References 41 publications

(36 reference statements)

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“…The CRISPR/Cas system is adventitious compared to ZFNs and TALENs in terms of simple designing, versatility, cost-effective, higher efficiency, multiplexing, and specificity [62]. Since the adaptation of CRISPR/Cas systems for GE in eukaryotes [63,64,65,66], it has emerged as the most popular GE tool that is also described as nature’s toolbox and ‘magic wand’ of genome engineering [67,68]. Thus, primarily CRISPR-mediated GE tools and their applications in PM studies are discussed in the following sections.…”

Section: Modern Tools To Explore Pm Interactionsmentioning

confidence: 99%

Exploration of Plant-Microbe Interactions for Sustainable Agriculture in CRISPR Era

2019

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Plants and microbes are co-evolved and interact with each other in nature. Plant-associated microbes, often referred to as plant microbiota, are an integral part of plant life. Depending on the health effects on hosts, plant–microbe (PM) interactions are either beneficial or harmful. The role of microbiota in plant growth promotion (PGP) and protection against various stresses is well known. Recently, our knowledge of community composition of plant microbiome and significant driving factors have significantly improved. So, the use of plant microbiome is a reliable approach for a next green revolution and to meet the global food demand in sustainable and eco-friendly agriculture. An application of the multifaceted PM interactions needs the use of novel tools to know critical genetic and molecular aspects. Recently discovered clustered regularly interspaced short palindromic repeats (CRISPR)/Cas-mediated genome editing (GE) tools are of great interest to explore PM interactions. A systematic understanding of the PM interactions will enable the application of GE tools to enhance the capacity of microbes or plants for agronomic trait improvement. This review focuses on applying GE techniques in plants or associated microbiota for discovering the fundamentals of the PM interactions, disease resistance, PGP activity, and future implications in agriculture.

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Section: Modern Tools To Explore Pm Interactionsmentioning

confidence: 99%

Exploration of Plant-Microbe Interactions for Sustainable Agriculture in CRISPR Era

2019

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“…While the precise mechanism of repair used to integrate the ssODN sequence is still unclear, this process harbors both an HDR signature (precision integration of the template) and NHEJ (indel formation) repair outcomes depending on the model system used. Recent in vitro work has implicated the requirement for Rad51 activity 98-101 . While this method can often lead to higher rates of integration than traditional HR, it is typically deployed for integrating sequences of 50 base pairs or less 102 .…”

Section: Associated Biochemical Activities Critical For Precision Genmentioning

confidence: 99%

Precision gene editing technology and applications in nephrology

et al. 2018

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The expanding field of precision gene editing is empowering researchers to directly modify DNA. Gene editing is made possible using synonymous technologies: a DNA-binding platform to molecularly locate user-selected genomic sequences and an associated biochemical activity that serves as a functional editor. The advent of accessible DNA-targeting molecular systems, such as zinc-finger nucleases, transcription activator-like effectors (TALEs) and CRISPR-Cas9 gene editing systems, has unlocked the ability to target nearly any DNA sequence with nucleotide-level precision. Progress has also been made in harnessing endogenous DNA repair machineries, such as non-homologous end joining, homology-directed repair and microhomology-mediated end joining, to functionally manipulate genetic sequences. As understanding of how DNA damage results in deletions, insertions and modifications increases, the genome becomes more predictably mutable. DNA-binding platforms such as TALEs and CRISPR can also be used to make locus-specific epigenetic changes and to transcriptionally enhance or suppress genes. Although many challenges remain, the application of precision gene editing technology in the field of nephrology has enabled the generation of new animal models of disease as well as advances in the development of novel therapeutic approaches such as gene therapy and xenotransplantation.

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“…So far, we have published many reviews regarding genome editing containing the newest information available at the time of each publication. For example, a general outline of this technology including historical background was reviewed in 2014, a more focused review on CRISPR‐Cas9 was published in 2015, and a comprehensive overview of transcription activator‐like effector (TALE) nuclease (TALEN) systems, updates on CRISPR tools, and recent advances on gene knock‐in systems were summarized in 2017. Within these reviews, we have introduced various achievements in technological development including our highly active variant of TALE/TALEN, named Platinum TALE/TALEN (Figure A) …”

Section: Genome Editing Reloaded: Attractive Derivatives Come Of Agementioning

confidence: 99%

Acceleration of cancer science with genome editing and related technologies

Sakuma

Yamamoto

2018

Cancer Science

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Genome editing includes various edits of the genome, such as short insertions and deletions, substitutions, and chromosomal rearrangements including inversions, duplications, and translocations. These variations are based on single or multiple DNA double‐strand break (DSB)‐triggered in cellulo repair machineries. In addition to these “conventional” genome editing strategies, tools enabling customized, site‐specific recognition of particular nucleic acid sequences have been coming into wider use; for example, single base editing without DSB introduction, epigenome editing with recruitment of epigenetic modifiers, transcriptome engineering using RNA editing systems, and in vitro detection of specific DNA and RNA sequences. In this review, we provide a quick overview of the current state of genome editing and related technologies that multilaterally contribute to cancer science.

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Magic wands of CRISPR—lots of choices for gene knock-in

Cited by 25 publications

References 41 publications

Exploration of Plant-Microbe Interactions for Sustainable Agriculture in CRISPR Era

Exploration of Plant-Microbe Interactions for Sustainable Agriculture in CRISPR Era

Precision gene editing technology and applications in nephrology

Acceleration of cancer science with genome editing and related technologies

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