Magic ferritin: A novel chemotherapeutic encapsulation bullet

Şimşek, Ece; Kılıç, Mehmet

doi:10.1016/j.jmmm.2005.01.066

Cited by 64 publications

(32 citation statements)

References 12 publications

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“…Although apoferritin has been used as the carrier of chemo drugs, 24,25 no evidence has been produced to show that the obtained protein-drug complex has comparable, let alone better, anticancer efficiency than the pristine drug. In addition, small drug molecules such as 5-FU could not be effectively loaded by apoferritin alone because 5-FU would readily leak from the cavity (see a more detailed description in ESI †).…”

Section: -13mentioning

confidence: 99%

Bio-inspired protein–gold nanoconstruct with core–void–shell structure: beyond a chemo drug carrier

Liu¹,

Wei²,

Huang³

et al. 2013

J. Mater. Chem. B

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Chemotherapy has been widely used in clinical practice for cancer treatment. A major challenge for a successful chemotherapy is to potentiate the anticancer activity, whilst reducing the severe side effects. In this context, we design a bio-inspired protein-gold nanoconstruct (denoted as AFt-Au hereafter) with a core-void-shell structure which exhibits a high selectivity towards carcinoma cells. Anticancer drug 5-fluorouracil (5-FU) can be sequestered into the void space of the construct to produce an integrated nanoscale hybrid AFt-AuFU that exhibits an increased cellular uptake of 5-FU. More importantly, AFt-Au, serving as a bio-nano-chemosensitizer, renders carcinoma cells more susceptible to 5-FU by cell-cycle regulation, and thus, leads to a dramatic decrease of the IC 50 value (i.e. the drug concentration required to kill 50% of the cell population) of 5-FU in HepG2 cells from 138.3 mM to 9.2 mM. Besides HepG2 cells, a remarkably enhanced anticancer efficacy and potentially reduced side effects are also achieved in other cell lines. Our further work reveals that the drug 5-FU is internalized into cells with AFt-Au primarily via receptor-mediated endocytosis (RME). After internalization, AFt-AuFU colocalizes with lysosomes which trigger the release of 5-FU under acidic conditions. Overall, our approach provides a novel procedure in nanoscience that promises an optimal chemotherapeutic outcome.Scheme 1 Design and construction of the AFt-AuFU nanoconstruct. AFt-Au was prepared by reducing AuCl 4À ions with NaBH 4 in the cavity of apoferritin. 5-FU was sequestered into the resulting void space of the AFt-Au complex by passive diffusion through the molecular channels in the apoferritin shell to produce a drug-loaded hybrid nanoconstruct (AFt-AuFU).

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Section: -13mentioning

confidence: 99%

Bio-inspired protein–gold nanoconstruct with core–void–shell structure: beyond a chemo drug carrier

Liu¹,

Wei²,

Huang³

et al. 2013

J. Mater. Chem. B

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“…It has been shown that this DFO-loaded apoferritin could react with The same procedure has also been used for the encapsulation of the marker fluorescein, [269] and the drugs doxorubicin, [268] cisplatin [270] and carboplatin [270] which were incorporated by adding the drugs to apoferritin at pH Although the efficiency of the reassembly procedure has been shown for diverse molecules, it is important to re-iterate that this encapsulation method has not been previously achieved for the entrapment of quantum dots within the apoferritin cavity.…”

Section: Entrapment Of Materials In Apoferritinmentioning

confidence: 99%

Aqueous Near‐Infrared Fluorescent Composites Based on Apoferritin‐Encapsulated PbS Quantum Dots

et al. 2008

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“…[12][13][14] Ferritins convert soluble ferrous salts to a ferric complex, and the latter is stored within the internal cavity as magnetic nanocores. 15,16 T 2 contrast enhancements by endogenous ferritin were used to estimate iron deposition in liver, pancreas, and spleen in healthy or diseased states.…”

mentioning

confidence: 99%

Iron-based ferritin nanocore as a contrast agent

Sana

Johnson

Sheah³

et al. 2010

Biointerphases

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Self-assembling protein cages have been exploited as templates for nanoparticle synthesis. The ferritin molecule, a protein cage present in most living systems, stores excess soluble ferrous iron in the form of an insoluble ferric complex within its cavity. Magnetic nanocores formed by loading excess iron within an engineered ferritin from Archaeoglobus fulgidus (AfFtn-AA) were studied as a potential magnetic resonance (MR) imaging contrast agent. The self-assembly characteristics of the AfFtn-AA were investigated using dynamic light scattering technique and size exclusion chromatography. Homogeneous size distribution of the assembled nanoparticles was observed using transmission electron microscopy. The magnetic properties of iron-loaded AfFtn-AA were studied using vibrating sample magnetometry. Images obtained from a 3.0 T whole-body MRI scanner showed significant brightening of T1 images and signal loss of T2 images with increased concentrations of iron-loaded AfFtn-AA. The analysis of the MR image intensities showed extremely high R2 values (5300 mM−1 s−1) for the iron-loaded AfFtn-AA confirming its potential as a T2 contrast agent.

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Magic ferritin: A novel chemotherapeutic encapsulation bullet

Cited by 64 publications

References 12 publications

Bio-inspired protein–gold nanoconstruct with core–void–shell structure: beyond a chemo drug carrier

Bio-inspired protein–gold nanoconstruct with core–void–shell structure: beyond a chemo drug carrier

Aqueous Near‐Infrared Fluorescent Composites Based on Apoferritin‐Encapsulated PbS Quantum Dots

Iron-based ferritin nanocore as a contrast agent

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