Magic angle sample spinning NMR spectroscopy of liquids as a nondestructive method for studies of plant seeds

Rutar, V.

doi:10.1021/jf00085a016

Cited by 46 publications

(38 citation statements)

References 10 publications

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“…2A and 2B, respectively), contributed undesirable peaks that obscured important portions of the seed oil spectrum. Conway and Johnson (8) found that performing spectra on samples covered with Freon TM 113 improved the spectra although other researchers have not used this technique (3)(4)(5)(6)(7). Results from this study indicated that it was necessary to immerse the seeds in solvent to obtain the best possible spectra while the choice of solvent was dependent on other factors.…”

Section: Resultsmentioning

confidence: 88%

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Practical nuclear magnetic resonance analysis of liquid oil in oilseeds: I factors affecting peak width

Reaney

Tyler

Brown

1999

J Americ Oil Chem Soc

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If proton nuclear magnetic resonance (NMR) spectra of single seeds can be improved, a rapid, low-cost method of screening seeds for oil composition could be developed for use as a selection tool in plant breeding. NMR spectroscopy was performed on single seeds of borage, flax, and canola to evaluate methods for improving spectra quality (narrowing peak widths and increasing signal-to-noise ratio) to a degree necessary to measure differences among seeds in a breeding program. Immersion of seeds in a variety of solvents, including deuterated chloroform, deuterated acetone, deuterated dimethyl sulfoxide (DMSO) and completely fluorinated hydrocarbons (FC-77), narrowed peaks obtained from seeds when compared with spectra from seeds analyzed in the absence of a solvent. Deuterated chloroform and FC-77 were free of interfering solvent proton peaks while deuterated acetone and deuterated DMSO contributed interfering peaks. The spectra of dehulled seeds had narrower peak widths than did seeds with hulls. Treatments that decreased seed oil viscosity failed to substantially narrow spectral peak widths of seeds. High magnetic field strength did not improve the spectral quality of seeds, as peak widths increased with field strength. Conversely, low field strength limited resolution of oil spectra. Although the 300 MHz spectrum of vegetable oil had greater resolution (narrower peaks) than the 60 MHz spectrum, spectra of seeds produced at 60 MHz had superior resolution to 300 MHz spectra.Paper no. J8831 in JAOCS 76, 859-862 (July 1999).

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Section: Resultsmentioning

confidence: 88%

“…In many seeds the only proton signal measurable by standard proton nuclear magnetic resonance (NMR) originates from the liquid oil (3)(4)(5). Unfortunately, solid seed materials broaden the spectral peak widths and limit the useful information derived from the NMR spectrum of a single seed.…”

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confidence: 99%

Practical nuclear magnetic resonance analysis of liquid oil in oilseeds: I factors affecting peak width

Reaney

Tyler

Brown

1999

J Americ Oil Chem Soc

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“…In some cases, relative seed oil content was also measured by magic angle sample spinning 1 H-NMR, according to the method of Rutar (1989). Analyses were conducted with 50-seed samples of intact wild type and AS11 seeds using a Bruker AM wide-bore spectrometer (Bruker Analytische Masstechnik, Silberstreifen, Germany) operating at 360 MHz.…”

Section: Lipid Analyses In As11 Wt and Dgat Transgenic Linesmentioning

confidence: 99%

Seed-Specific Over-Expression of an Arabidopsis cDNA Encoding a Diacylglycerol Acyltransferase Enhances Seed Oil Content and Seed Weight

et al. 2001

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We recently reported the cloning and characterization of an Arabidopsis (ecotype Columbia) diacylglycerol acyltransferase cDNA (Zou et al., 1999) and found that in Arabidopsis mutant line AS11, an ethyl methanesulfonate-induced mutation at a locus on chromosome II designated as Tag1 consists of a 147-bp insertion in the DNA, which results in a repeat of the 81-bp exon 2 in the Tag1 cDNA. This insertion mutation is correlated with an altered seed fatty acid composition, reduced diacylglycerol acyltransferase (DGAT; EC 2.3.1.20) activity, reduced seed triacylglycerol content, and delayed seed development in the AS11 mutant. The effect of the insertion mutation on microsomal acyl-coenzyme A-dependent DGAT is examined with respect to DGAT activity and its substrate specificity in the AS11 mutant relative to wild type. We demonstrate that transformation of mutant AS11 with a single copy of the wild-type Tag1 DGAT cDNA can complement the fatty acid and reduced oil phenotype of mutant AS11. More importantly, we show for the first time that seed-specific over-expression of the DGAT cDNA in wild-type Arabidopsis enhances oil deposition and average seed weight, which are correlated with DGAT transcript levels. The DGAT activity in developing seed of transgenic lines was enhanced by 10% to 70%. Thus, the current study confirms the important role of DGAT in regulating the quantity of seed triacylglycerols and the sink size in developing seeds.

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“…It is known that if a sample is spun mechanically, at a rate faster than the spectral broadening originating from these interactions, and at the ''magic angle'' of 54°44Ј (which meets the criterion of 3 cos 2 (54°44Ј) Ϫ 1 ϭ 0), the contribution from these interactions to the MR spectral broadening can be significantly reduced (12)(13)(14)(15). In biological studies, MAS 1 HMR has been demonstrated to be useful in the noninvasive analyses of plant material and lipids (16)(17)(18)(19)(20). Applying MAS on human͞animal tissue should reduce the effect of residual molecular interactions and the influence of magnetic susceptibility on spectral broadening.…”

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confidence: 99%

Quantitative neuropathology by high resolution magic angle spinning proton magnetic resonance spectroscopy

Cheng

J²,

Becerra³

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

330

344

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We describe a method that directly relates tissue neuropathological analysis to medical imaging. Presently, only indirect and often tenuous relationships are made between imaging (such as MRI or x-ray computed tomography) and neuropathology. We present a biochemistry-based, quantitative neuropathological method that can help to precisely quantify information provided by in vivo proton magnetic resonance spectroscopy (

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Magic angle sample spinning NMR spectroscopy of liquids as a nondestructive method for studies of plant seeds

Cited by 46 publications

References 10 publications

Practical nuclear magnetic resonance analysis of liquid oil in oilseeds: I factors affecting peak width

Practical nuclear magnetic resonance analysis of liquid oil in oilseeds: I factors affecting peak width

Seed-Specific Over-Expression of an Arabidopsis cDNA Encoding a Diacylglycerol Acyltransferase Enhances Seed Oil Content and Seed Weight

Quantitative neuropathology by high resolution magic angle spinning proton magnetic resonance spectroscopy

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