Macrophage Migration Inhibitory Factor Promotes Expression of Matrix Metalloproteinases 1 and 3 in Spinal Cord Astrocytes following Gecko Tail Amputation

Zhang, Haijun; Sun, Chunshuai; He, Bin; Zhang, Xingyuan; Hao, Huifei; Hou, Yuxuan; Li, Aicheng; Wang, Yongjun; Wang, Yingjie

doi:10.31083/j.jin2202029

Cited by 5 publications

(3 citation statements)

References 75 publications

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“…We describe here a clodronate-induced accumulation of both collagen and myofibroblasts. Based on studies in other model organisms, newt macrophages could be directly impacting extracellular matrix remodeling [ 19 , 102 , 116 – 119 ]. Our findings suggest that under normal conditions in the newt eye, macrophages play a critical role in preventing myofibroblast accumulation and modulating ECM remodeling.…”

Section: Discussionmentioning

confidence: 99%

Macrophages modulate fibrosis during newt lens regeneration

Tsissios,

Sallese,

Perez-Estrada

et al. 2024

Stem Cell Res Ther

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Background Previous studies have suggested that macrophages are present during lens regeneration in newts, but their role in the process is yet to be elucidated. Methods Here we generated a transgenic reporter line using the newt, Pleurodeles waltl, that traces macrophages during lens regeneration. Furthermore, we assessed early changes in gene expression during lens regeneration using two newt species, Notophthalmus viridescens and Pleurodeles waltl. Finally, we used clodronate liposomes to deplete macrophages during lens regeneration in both species and tested the effect of a subsequent secondary injury after macrophage recovery. Results Macrophage depletion abrogated lens regeneration, induced the formation of scar-like tissue, led to inflammation, decreased iris pigment epithelial cell (iPEC) proliferation, and increased rates of apoptosis in the eye. Some of these phenotypes persisted throughout the last observation period of 100 days and could be attenuated by exogenous FGF2 administration. A distinct transcript profile encoding acute inflammatory effectors was established for the dorsal iris. Reinjury of the newt eye alleviated the effects of macrophage depletion, including the resolution of scar-like tissue, and re-initiated the regeneration process. Conclusions Together, our findings highlight the importance of macrophages for facilitating a pro-regenerative environment in the newt eye by regulating fibrotic responses, modulating the overall inflammatory landscape, and maintaining the proper balance of early proliferation and late apoptosis of the iPECs.

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Section: Discussionmentioning

confidence: 99%

Macrophages modulate fibrosis during newt lens regeneration

Tsissios,

Sallese,

Perez-Estrada

et al. 2024

Stem Cell Res Ther

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“…Twenty-eight kinds of MMP have been found so far, with MMP-1, -3, and -13 playing a central role [57]. MMP-3 can degrade a broad spectrum of ECM components, including collagen, PG, laminins, and fibronectin [58]. The MMP varieties do not exist normally.…”

Section: Discussionmentioning

confidence: 99%

Nuclear Factor-κB Decoy Oligodeoxynucleotide Attenuates Cartilage Resorption In Vitro

Nemoto,

Sakai,

Watson

et al. 2024

Bioengineering

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Background: Cartilage harvest and transplantation is a common surgery using costal, auricular, and septal cartilage for craniofacial reconstruction. However, absorption and warping of the cartilage grafts can occur due to inflammatory factors associated with wound healing. Transcription factor nuclear factor-κB (NF-κB) is activated by the various stimulation such as interleukin-1 (IL-1), and plays a central role in the transactivation of this inflammatory cytokine gene. Inhibition of NF-κB may have anti-inflammatory effects. The aim of this study was to explore the potential of an NF-κB decoy oligodeoxynucleotide (Decoy) as a chondroprotective agent. Materials and Methods: Safe and efficacious concentrations of Decoy were assessed using rabbit nasal septal chondrocytes (rNSChs) and assays for cytotoxicity, proteoglycan (PG) synthesis, and PG turnover were carried out. The efficacious concentration of Decoy determined from the rNSChs was then applied to human nasal septal cartilage (hNSC) in vitro and analyzed for PG turnover, the levels of inflammatory markers, and catabolic enzymes in explant-conditioned culture medium. Results: Over the range of Decoy conditions and concentrations, no inhibition of PG synthesis or cytotoxicity was observed. Decoy at 10 μM effectively inhibited PG degradation in the hNSC explant, prolonging PG half-life by 63% and decreasing matrix metalloprotease 3 (MMP-3) by 70.7% (p = 0.027). Conclusions: Decoy may be considered a novel chondroprotective therapeutic agent in cartilage transplantation due to its ability to inhibit cartilage degradation due to inflammation cytokines.

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“…The importance of MMPs in protein degradation can be evidenced by their wide distribution in different groups of vertebrates [44], including mammals [6][7][8][9], birds [45], reptiles [46,47], amphibians [48] and fish [10], as well as insects [49] and echinoderms [50].…”

Section: Discussionmentioning

confidence: 99%

Metalloproteinases in Restorative Dentistry: An In Silico Study toward an Ideal Animal Model

de Oliveira,

Kotowski,

Sampaio-Filho

et al. 2023

Biomedicines

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In dentistry, various animal models are used to evaluate adhesive systems, dental caries and periodontal diseases. Metalloproteinases (MMPs) are enzymes that degrade collagen in the dentin matrix and are categorized in over 20 different classes. Collagenases and gelatinases are intrinsic constituents of the human dentin organic matrix fibrillar network and are the most abundant MMPs in this tissue. Understanding such enzymes’ action on dentin is important in the development of approaches that could reduce dentin degradation and provide restorative procedures with extended longevity. This in silico study is based on dentistry’s most used animal models and intends to search for the most suitable, evolutionarily close to Homo sapiens. We were able to retrieve 176,077 mammalian MMP sequences from the UniProt database. These sequences were manually curated through a three-step process. After such, the remaining 3178 sequences were aligned in a multifasta file and phylogenetically reconstructed using the maximum likelihood method. Our study inferred that the animal models most evolutionarily related to Homo sapiens were Orcytolagus cuniculus (MMP-1 and MMP-8), Canis lupus (MMP-13), Rattus norvegicus (MMP-2) and Orcytolagus cuniculus (MMP-9). Further research will be needed for the biological validation of our findings.

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Macrophage Migration Inhibitory Factor Promotes Expression of Matrix Metalloproteinases 1 and 3 in Spinal Cord Astrocytes following Gecko Tail Amputation

Cited by 5 publications

References 75 publications

Macrophages modulate fibrosis during newt lens regeneration

Macrophages modulate fibrosis during newt lens regeneration

Nuclear Factor-κB Decoy Oligodeoxynucleotide Attenuates Cartilage Resorption In Vitro

Metalloproteinases in Restorative Dentistry: An In Silico Study toward an Ideal Animal Model

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