Macrophage lineage phenotypes and osteoclastogenesis—Complexity in the control by GM-CSF and TGF-β

Lari, Roya; Fleetwood, Andrew J.; Kitchener, PD; Cook, Andrew D.; Pavasovic, Durda; Hertzog, Paul J.; Hamilton, John A.

doi:10.1016/j.bone.2006.09.003

Cited by 78 publications

(83 citation statements)

References 72 publications

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“…Given the context of wearparticle-induced osteolysis, these widely used populations in macrophage lineage biology were chosen in part because they can give rise to osteoclasts, the BMM in particular being able to be differentiated most efficiently into osteoclasts. 32 Previous studies with these particles observed toxic effects on macrophages, 35 which we also observed but at high concentrations. Thus careful dose-response protocols are important in studies of macrophage lineage populations and wear particles.…”

Section: Discussionsupporting

confidence: 59%

“…In its absence they gradually die by apoptosis. 16,31,32 Uptake of TiAlV or CoCr particles also prevents their cell death; dose response studies determined the optimal concentration for preventing cell death to be $10 7 particles/mL, with lower concentrations ( 10 6 particles/mL) being less effective, and a higher concentration (10 8 particles/mL) showing toxic effects (Fig. 1a).…”

Section: Resultsmentioning

confidence: 99%

“…31,32 Progenitors were cultured in RPMI supplemented with 10% fetal calf serum, penicillin/ streptomycin, L-glutamine, and 5,000 U/mL human CSF-1 (Chiron Corp., Emeryville, CA), and adherent cells grown to confluence in 24-well plates (Iwaki, ATG, Tokyo).…”

Section: Reagentsmentioning

confidence: 99%

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Low dose metal particles can induce monocyte/macrophage survival

Lacey

Kok

Clanchy

et al. 2009

Journal Orthopaedic Research

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Aseptic loosening results in pain, loss of function, and ultimately prosthetic joint failure and revision surgery. The generation of wear particles from the prosthesis is a major factor in local osteolysis. We investigated the effects of such wear particles on the survival of monocytes and macrophages, populations implicated in wear particle-driven pathology. Particles from titanium aluminum vanadium (TiAlV) and cobalt chromium (CoCr) alloys were generated in-house and were equivalent in size (0.5-3 mm) to those seen in patients. Human CD14 þ monocytes and murine bone marrow-derived macrophages (BMM) were treated with TiAlV and CoCr particles in vitro, and cell survival was assayed. Both particles increased monocyte and macrophage survival in a dose-dependent manner, with an optimal concentration of around 10 7 particles/mL. Conditioned media from particle-treated BMM also increased macrophage survival. Studies with antibody blockade and gene-deficient mice suggest that particle-induced BMM survival is independent of endogenous CSF-1 (M-CSF), GM-CSF, and TNFa. These data indicate that wear particles can promote monocyte/macrophage survival in vitro possibly via an endogenous mediator. If this phenomenon occurs in vivo, it could mean that increased numbers of macrophages (and osteoclasts) would be found at a site of joint implant failure, which could contribute to the local inflammatory reaction and osteolysis. ß

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Section: Discussionsupporting

confidence: 59%

Section: Resultsmentioning

confidence: 99%

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Low dose metal particles can induce monocyte/macrophage survival

Lacey

Kok

Clanchy

et al. 2009

Journal Orthopaedic Research

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“…However, it can act on macrophage populations and are implicated in host defense and inflammation [3]. GM-CSF can generate a population of Granulocyte Monocyte-bone marrow-derived macrophages (GM-BMM) [4,5] that have macrophage and dendritic cell (DC) properties and are often employed as a model for an immature DC [6,7].…”

Section: Introductionmentioning

confidence: 99%

Impact of Serum Granulocyte-Macrophage Colony-Stimulating Factor Levels among Diabetes Patients in Hilla City - Iraq

Al-Hassnawi¹,

Al-Thaheb²,

Al-Dahmoshi³

et al. 2014

als

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Background: Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a pro-inflammatory cytokine with growth factor-like properties for monocytes and dendritic cells. Methods: Study subjects were recruited from the hospital in Hilla city. Healthy controls (n=15) and D.M. patients (n=35) were included in the study. Serum levels of GM-CSF and Anti-Insulin Antibody (AIA) measured by using ELISA technique. Results: The serum GM-CSF levels were significantly higher among diabetes subjects as compared with subjects without diabetes and results showed a high significant in serum AIA and glucose levels in patient as compare with control (p=0.009, P=0.001). The serum GM-CSF levels were increased in age group (45-54 years) shows the higher percent (42.85%) of patients with diabetes, with decreased of AIA and GM-CSF level rather than other age groups. Conclusions: There is indirect relationship between AIA and GM-CSF level and that is mean increased AIA and glucose level reflect the reduced GM-CSF level among the diabetic patients may be used as biomarker or use GM-CSF as co-therapy with insulin.

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“…A major effect of CSF-1 on human bone marrow cells and monocytes in vitro, as well as on blast cells of acute myeloid leukemia, is differentiation rather than proliferation (13, 35); we have reported that during murine osteoclastogenesis CSF-1 seems to provide a differentiation signal (36). In addition, there are in vivo and in vitro data indicating that CSF-1 can negatively regulate both the maintenance and commitment of multipotent precursor cells into other lineages by promoting macrophage lineage development (37,38).…”

Section: Discussionmentioning

confidence: 99%