Macrolide Resistance and
            <i>In Vitro</i>
            Potentiation by Peptidomimetics in Porcine Clinical Escherichia coli

Ma, Yan-Hui; Pirolo, Mattia; Subramani, Prabha; Gehring, Ronette; Damborg, Peter; Franzyk, Henrik; Guardabassi, Luca

doi:10.1128/msphere.00402-22

Cited by 8 publications

(6 citation statements)

References 35 publications

(41 reference statements)

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“…In general, most classes of ARGs in swine feces are those that are resistant to macrolide, peptide, tetracycline, and nitroimidazole. These resistance classes of genes are more common in swine farms worldwide, including those in China, the United States, and Europe, and are used to treat infections with pathogens such as E. coli, Streptococcus, and Staphylococcus aureus [25][26][27]. This can be partly explained by the fact that these classes of antibiotics have been used in pig production for a long time, despite geographic and breed differences.…”

Section: Discussionmentioning

confidence: 99%

Genetic- and Fiber-Diet-Mediated Changes in Antibiotic Resistance Genes in Pig Colon Contents and Feces and Their Driving Factors

Wang,

Luo,

Kong

et al. 2023

Microorganisms

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Comprehensive studies on the effects of genetics and fiber diets on antibiotic resistance genes (ARGs) remain scarce. In this study, we analyzed the profiles of ARGs in colonic contents and fecal samples of Taoyuan, Duroc, and Xiangcun pigs (n = 10) fed at different fiber levels. Through macrogenomic analysis, we identified a total of 850 unique types of ARGs and classified them into 111 drug resistance classes. The abundance of partially drug-resistant ARGs was higher in the colonic contents of local pig breeds under a large-scale farming model. ARGs were found to be widely distributed among a variety of bacteria, predominantly in the phyla Firmicutes, Proteobacteria, and Bacteroidetes. Fiber diets reduce the abundance of ARGs in colonic contents and feces, and mobile genetic elements (MGEs) and short-chain fatty acids (SCFAs) are important drivers in mediating the effect of fiber diets on the abundance of ARGs. In vitro fermentation experiments confirmed that butyric acid significantly reduced the abundance of ARGs. In summary, the results of this study enhanced our understanding of the distribution and composition of ARGs in the colon of different breeds of pigs and revealed that a fiber diet can reduce ARGs in feces through its Butyric acid, providing reference data for environmental safety.

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Section: Discussionmentioning

confidence: 99%

Genetic- and Fiber-Diet-Mediated Changes in Antibiotic Resistance Genes in Pig Colon Contents and Feces and Their Driving Factors

Wang,

Luo,

Kong

et al. 2023

Microorganisms

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“…Kit was often used to treat bacterial infections because of its broad bactericidal ability, such as Gram-positive cocci infections, including Streptococcus pyogenes and Staphylococcus aureus . This type of antibiotic was extensively used in agriculture as growth-promoting additive for swine and poultry. , Thus, antibiotic resistance in association with Kit resistance was reported in Brachyspira hyodysenteriae, Chromobacterium violaceum, and E. coli .…”

Section: Discussionmentioning

confidence: 99%

“…46,47 Thus, antibiotic resistance in association with Kit resistance was reported in Brachyspira hyodysenteriae, 48 Chromobacterium violaceum, 33 and E. coli. 46 The resistant mechanism involved the resistant gene mccE that acetylated Kit and converted it into a nontoxic compound 49 or mutation on FhuD and FhuB, the target of Kit. 50 However, Kit was not widely used in the clinic since it does not have an advantage over other antibiotics.…”

Section: ■ Discussionmentioning

confidence: 99%

“…This type of antibiotic was extensively used in agriculture as growth-promoting additive for swine and poultry. , Thus, antibiotic resistance in association with Kit resistance was reported in Brachyspira hyodysenteriae, Chromobacterium violaceum, and E. coli . The resistant mechanism involved the resistant gene mccE that acetylated Kit and converted it into a nontoxic compound or mutation on FhuD and FhuB, the target of Kit .…”

Section: Discussionmentioning

confidence: 99%

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Functional Proteomics Analysis of Norfloxacin-Resistant Edwardsiella tarda

Wu,

Li,

Tan

et al. 2023

J. Proteome Res.

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Multidrug-resistant Edwardsiella tarda threatens both sustainable aquaculture and human health, but the control measure is still lacking. In this study, we adopted functional proteomics to investigate the molecular mechanism underlying norfloxacin (NOR) resistance in E. tarda. We found that E. tarda had a global proteomic shift upon acquisition of NOR resistance, featured with increased expression of siderophore biosynthesis and Fe3+-hydroxamate transport. Thus, either inhibition of siderophore biosynthesis with salicyl-AMS or treatment with another antibiotic, kitasamycin (Kit), which was uptake through Fe3+-hydroxamate transport, enhanced NOR killing of NOR-resistant E. tarda both in vivo and in vitro. Moreover, the combination of NOR, salicyl-AMS, and Kit had the highest efficacy in promoting the killing effects of NOR than any drug alone. Such synergistic effect not only confirmed in vitro and in vivo bacterial killing assays but also applicable to other clinic E. tarda isolates. Thus, our data suggest a proteomic-based approach to identify potential targets to enhance antibiotic killing and propose an alternative way to control infection of multidrug-resistant E. tarda.

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“…Azithromycin has been identified as the most potent macrolide against E. coli. Given the scarcity of new antimicrobials capable of fighting Gram-negative infections, the scientific community has shown interest in repurposing macrolides (Ma et al 2022).…”

Section: Discussionmentioning

confidence: 99%

Abundance and phenotypic-genotypic analysis of antibiotic-resistant Escherichia coli isolated from wastewater of the Zainoel Abidin Hospital, Banda Aceh, Indonesia

SUHARTONO

Hayati

2023

Biodiversitas

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Abstract. Anelia N, Suhartono S, Hayati Z. 2023. Abundance and phenotypic-genotypic analysis of antibiotic-resistant Escherichia coli isolated from wastewater of the Zainoel Abidin Hospital, Banda Aceh, Indonesia. Biodiversitas 24: 2294-2301. Excessive and inappropriate use of antibiotics can initiate the emergence of antibiotic resistance in bacteria. Wastewater Treatment Plant (WWTP) is believed to be a reservoir for antibiotic-resistant bacteria, including Escherichia coli. This study aimed to perform the enumeration, isolation, characterization, and evaluation of resistance properties and antibiotic resistance-coding genes encoded by a plasmid of E. coli isolates from WWTP of the dr. Zainoel Abidin General Hospital (RSUDZA). The study was conducted through several stages, including the isolation and quantification of E. coli, antibiotic resistance testing, confirmation testing of ESBL-E. coli isolates, isolation of plasmid DNA, and amplification of TEM, SHV, and CTX-M genes. The results showed that the wastewater in the sum pit tank, aeration tank, and final tank of the WWTP contained E. coli at 7.5x104 CFU/mL, 3x102 CFU/mL, and 1x102 CFU/mL, respectively. A total of 22 isolates were obtained, with ten isolates from the sum pit tank, seven from the aeration tank, and five from the final tank. Escherichia coli isolates had resistance to eight types of antibiotics in the aeration tank and seven types in the final tank, compared to the sum pit tank of the WWTP. Therefore, 59% of the isolates were classified as Multi-drug Resistant (MDR) and 32% as Extended Spectrum ?-Lactamase E. coli (ESBL-EC). TEM, SHV, and CTX-M genes were detected in all locations at 63.6%, 22.7%, and 95.5%, respectively. In addition, CTX-M was the most common gene found in wastewater isolates at RSUDZA WWTP. The hospital wastewater treatment process must be improved to reduce the presence of antibiotic-resistant bacteria and the spread of antibiotic resistance genes.

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Macrolide Resistance and In Vitro Potentiation by Peptidomimetics in Porcine Clinical Escherichia coli

Cited by 8 publications

References 35 publications

Genetic- and Fiber-Diet-Mediated Changes in Antibiotic Resistance Genes in Pig Colon Contents and Feces and Their Driving Factors

Genetic- and Fiber-Diet-Mediated Changes in Antibiotic Resistance Genes in Pig Colon Contents and Feces and Their Driving Factors

Functional Proteomics Analysis of Norfloxacin-Resistant Edwardsiella tarda

Abundance and phenotypic-genotypic analysis of antibiotic-resistant Escherichia coli isolated from wastewater of the Zainoel Abidin Hospital, Banda Aceh, Indonesia

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