Macrobrachium rosenbergii mannose binding lectin: Synthesis of MrMBL-N20 and MrMBL-C16 peptides and their antimicrobial characterization, bioinformatics and relative gene expression analysis

Arockiaraj, Jesu; Chaurasia, Mukesh Kumar; Kumaresan, Venkatesh; Palanisamy, R.; Harikrishnan, Ramasamy; Pasupuleti, Mukesh; Kasi, Marimuthu

doi:10.1016/j.fsi.2014.12.036

Cited by 40 publications

(18 citation statements)

References 71 publications

(58 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It has been proposed that probiotic bacteria, the main components of synbiotics, can interact with some pattern recognition receptors (PRRs), such as Toll‐like receptors (TLRs), and upregulate specific cytokines, thereby exaggerating the fish immune response (Yang et al, ). Recently, evidence of cell recognition by mannose‐binding lectin (MBL) following synbiotic supplementation has also been reported (Arockiaraj et al, ). The previous findings of Abid et al () revealing marked increases in pro‐inflammatory cytokines in both the anterior and posterior intestine of Atlantic salmon after synbiotic administration could support this mechanism.…”

Section: Discussionmentioning

confidence: 99%

Effect of dietary supplementation with a synbiotic (Lacto Forte) on growth performance, haematological and histological profiles, the innate immune response and resistance to bacterial disease inOreochromis niloticus

et al. 2019

View full text Add to dashboard Cite

This study investigated the effects of a synbiotic Lacto Forte on growth performance, haemato-immunological responses, plasma bactericidal capacity, histological profiles and resistance of Oreochromis niloticus to Pseudomonas fluorescens. The experimental fish were divided into three groups; two of them (T1 and T2) were given Lacto Forte incorporated diets at increasing level (0.7 and 1.5 g/kg, respectively) and an additive-free basal diet served as the control (T0) for 30 and 45 days. At the end of the feeding trial, a challenge was performed using a virulent strain of P. fluorescens and mortalities were recorded over an additional 14-days period. The results showed that Lacto Forte has a pronounced effect on haematological and growth performance parameters at 1.5 g/kg rather than 0.5 g/kg (p < 0.05). Plasma lysozyme, proteases, antiproteases and bactericidal capacity were significantly enhanced (p < 0.05) in Lacto Forte-treated groups, particularly after 45 days of supplementation. Long-term supplementation with Lacto Forte (1.5 g/kg) induced degenerative changes in the liver, spleen and intestine. Additionally, a significant increase (p < 0.05) in survival rates were found in Lacto Forte-treated groups compared to the control one 14 days post-challenge. As a result, Lacto Forte fortifies tilapia immune response and can be used as a surrogate for antibiotics to control P. fluorescens. K E Y W O R D S bactericidal, growth performance, Innate immunity, Oreochromis niloticus, Pseudomonas fluorescens, synbiotic Lacto Forte How to cite this article: Ismail M, Wahdan A, Yusuf MS, Metwally E, Mabrok M. Effect of dietary supplementation with a synbiotic (Lacto Forte) on growth performance, haematological and histological profiles, the innate immune response and resistance to bacterial disease in Oreochromis niloticus. Aquac Res. 2019;50:2545-2562.

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of dietary supplementation with a synbiotic (Lacto Forte) on growth performance, haematological and histological profiles, the innate immune response and resistance to bacterial disease inOreochromis niloticus

et al. 2019

View full text Add to dashboard Cite

show abstract

“…), lectin (Arockiaraj et al . ) and catalase (Arockiaraj et al . ) in M. rosenbergii were studied, which can be used as biomarkers to detect the disease state of prawns in future.…”

Section: Immune Systemmentioning

confidence: 99%

Impacts of environmental and biological stressors on immune system ofMacrobrachium rosenbergii

Kumaresan

Palanisamy

Pasupuleti

et al. 2016

Reviews in Aquaculture

Self Cite

View full text Add to dashboard Cite

Macrobrachium rosenbergii commonly called giant freshwater prawn is a widely farmed crustacean in freshwater. Similar to other aquatic organisms, their growth and well-being is influenced by various physical, chemical and biological factors. We discuss about the critical growth limiting factors as well as disease causing agents and the potential immune molecules of M. rosenbergii that are proved to involve in preventing and/or responding to those limiting factors.

show abstract

“…Estas lectinas han sido descritas en varias especies de crustáceos Fenneropenaeus chinensis (Sun et al, 2008;Zhan et al, 2009;Xu et al, 2010) en Litopenaeus vannamei (Zhan et al, 2009a(Zhan et al, , 2009b. Song et al (2014) sugiere una interacción directa entre los MOS y el sistema inmune innato de crustáceos a través de algunos PRRs con afinidad por manosa (Sun et al, 2008;Arockiaraj et al, 2015), los cuales a su vez presentan alta especifidad de reconocimiento de PAMPs asociados a V. angillarum, E. coli y WSSV (Sun et al, 2008;Zhang et al, 2009aZhang et al, , 2009bZhao et al, 2009;Xu et al, 2010). Se ha sugerido que estos PRRs están generalmente vinculados a la vía del complemento (Arockiaraj et al, 2015;Clark & Greenwood, 2016), pero existen carencias investigativas sobre este aspecto en particular (Clark & Greenwood, 2016).…”

Section: Vías Documentadasunclassified

“…Song et al (2014) sugiere una interacción directa entre los MOS y el sistema inmune innato de crustáceos a través de algunos PRRs con afinidad por manosa (Sun et al, 2008;Arockiaraj et al, 2015), los cuales a su vez presentan alta especifidad de reconocimiento de PAMPs asociados a V. angillarum, E. coli y WSSV (Sun et al, 2008;Zhang et al, 2009aZhang et al, , 2009bZhao et al, 2009;Xu et al, 2010). Se ha sugerido que estos PRRs están generalmente vinculados a la vía del complemento (Arockiaraj et al, 2015;Clark & Greenwood, 2016), pero existen carencias investigativas sobre este aspecto en particular (Clark & Greenwood, 2016). Por otra parte, De Vrese & Schrezenmeir (2008) y Bindels et al (2015) plantean que las principales propiedades inmunomoduladoras de los prebióticos son indirectas, ya que resultan mediadas por los cambios promovidos en la composición y/o actividad de la microbiota intestinal.…”

Section: Vías Documentadasunclassified

Manano oligosacaridos como prebioticos en acuicultura de crustaceos

Gainza¹,

Romero²

2017

lajar

View full text Add to dashboard Cite

RESUMEN.Los prebióticos tienen el potencial de incrementar la eficiencia y sostenibilidad de la producción acuícola. Uno de los prebióticos más promisorios en acuicultura son los Manano oligosacáridos (MOS). Estos MOS son moléculas de carbohidratos complejos derivados de la pared de la célula de la levadura Saccharomyces cerevisiae, que impiden la adhesión de patógenos bacterianos porque actúan bloqueando la adherencia de las lectinas microbianas con los carbohidratos presentes en la superficie de las células intestinales. Esta prevención de la adhesión impide la colonización y proceso infectivo de los patógenos. La ventaja del uso de MOS es que puede prevenir infecciones a través de mecanismos diferentes a los utilizados por los antibióticos. Este artículo muestra los avances obtenidos en uso de MOS como prebióticos en la acuicultura, en particular en el cultivo de crustáceos, donde han generado respuestas positivas en todas las experiencias actuando sobre el incremento del crecimiento, la supervivencia, modulando la respuesta inmune y modificando la morfología del tracto digestivo. La identificación de las cepas que son promovidas o excluidas y la caracterización de la acción moduladora en la microbiota resultante quedan abiertas como uno de los campos a desarrollar en conjunto con la extensión de estos resultados a la práctica productiva. Palabras clave: crustáceos, manano oligosacáridos, MOS, prebióticos, microbiota, crecimiento. Mannan oligosaccharides as prebiotics in crustacean aquacultureABSTRACT. Probiotics have the potential to increase the efficiency and sustainability of aquaculture production. The mannan-oligosaccharides (MOS) are complex carbohydrates derived from the cell wall of the yeast Saccharomyces cerevisiae, which act by blocking adherence of bacterial lectins to carbohydrates on the surface of intestinal cells; this action prevents colonization and infection processes carried out by bacterial pathogens. In this work, we review the current evidence about the use of MOS as prebiotics in shellfish aquaculture, enlightening the positive responses in most of the experiences, such as improvement in growth and survival, modulating the immune response and modifying the morphology of the digestive tract. The identification of strains that are promoted or excluded, and the characterization of the resulting modulating action in gut´s microbiota, remains open as one of the fields to be developed in conjunction with the extension of these results to the productive practice.

show abstract

Macrobrachium rosenbergii mannose binding lectin: Synthesis of MrMBL-N20 and MrMBL-C16 peptides and their antimicrobial characterization, bioinformatics and relative gene expression analysis

Cited by 40 publications

References 71 publications

Effect of dietary supplementation with a synbiotic (Lacto Forte) on growth performance, haematological and histological profiles, the innate immune response and resistance to bacterial disease inOreochromis niloticus

Effect of dietary supplementation with a synbiotic (Lacto Forte) on growth performance, haematological and histological profiles, the innate immune response and resistance to bacterial disease inOreochromis niloticus

Impacts of environmental and biological stressors on immune system ofMacrobrachium rosenbergii

Manano oligosacaridos como prebioticos en acuicultura de crustaceos

Contact Info

Product

Resources

About