Machined titanium disc decontamination using photodynamic therapy: an in vitro study

Abstract

ObjectiveThis study investigated less invasive protocols that do not injure peri-implant tissues during implant surface decontamination and evaluated, in vitro, the efficacyof microbiological decontamination of machined surface titanium discs with photodynamic therapy.MethodsForty eight titanium disc contaminated with 10μL of a Streptococcus sanguinis suspension were randomly divided into groups: 1) titanium disc contaminated with Ss (titanium disc suspension) without treatment; 2) titanium disc suspension rinsed with saline solution; 3) titanium disc suspension rinsed with 0.2% chlorhexidine digluconate; 4) titanium disc suspension treated with Photosensitizer Methilene Blue; 5) titanium disc suspension treated with Photosensitizer Methilene Blue associated with laser diode; 6) titanium disc suspension treated with diode laser. After the treatments, the titanium disc were submersed in 3mL of sterile brain-heart infusion broth under aerobic conditions at 37ºC for 48 hours. Three petri plates were seeded per sample and maintained under aerobic conditions at 37ºC for 48 hours, after which the number of colony forming units per milliliter was counted.ResultsThe Kruskal-Wallis test complemented by Dunn test showed that chlorhexidine digluconate eliminated titanium disc contamination (p<0.05). All the other groups (2, 4, 5, 6) had fewer colony-forming units than group 1 (p<0.05).ConclusionWithin the limitations of this study, Photodynamic Therapy reduced titanium disc contamination but was not better than 0.2 % chlorhexidine digluconate rinsing.Indexing terms: Chlorhexidine. Implant. Laser. Titanium.

Effect of Photodynamic Therapy Using Toluidine Blue on Eikenella corrodens and Aggregatibacter actinomycetemcomitans Biofilms Adhered to Titanium Discs: An In Vitro Study

“…Another study was conducted by Biondi Filho et al in which the discs were contaminated with Streptococcus sanguinis suspension. (28) The results showed that CHX was very beneficial, and interestingly, microbial reduction in the methylene blue group alone was more than that in the aPDT-treated group. (28) In the cited study, the samples were contaminated with 10 µl of microbial suspension for one hour.…”

“…(28) The results showed that CHX was very beneficial, and interestingly, microbial reduction in the methylene blue group alone was more than that in the aPDT-treated group. (28) In the cited study, the samples were contaminated with 10 µl of microbial suspension for one hour. Also, the discs were much smaller than the discs used in the present study, and accordingly, decontamination with laser showed better results due to the higher concentration of irradiation in a smaller surface area.…”

“…(30) In studies by Marotti et al and Biondi Filho et al, the duration of contamination of the samples was reported to be 5 minutes with saliva and one hour with microbial suspensions, respectively. (23,28) In the research by Hauser-Gerspach et al, this duration was considered 2 hours. (29) This extended period can have a significant effect on the number and the volume of the microbes.…”