Machine vision based analysis and harvest of somatic embryos

Harrell, R. C.; Hood, C.F.; Moltó, Enrique; Munilla, R.; Bieniek, Marianne E.; Cantliffe, Daniel J.

doi:10.1016/0168-1699(93)90026-w

Cited by 14 publications

(8 citation statements)

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“…The feature set used in this study included many of those described in Harrell et al (1993) and seven new features which attempted to quantify key visual cues used by the expert when distinguishing between the two classes. It was noted that those objects classified by the expert as harvest were, in general, characterized with symmetry about the major or principal axis, asymmetry about the minor axis and had straight, uniformly sloped sides in the hypocotyl region near the object's center.…”

Section: The Embryo Classifiermentioning

confidence: 99%

“…1/lun ASYM_MAJ/ASYM_MIN, dimensionless Measure of the nonlinearity or irregularity of an object's contour near the centroid, dimensionless Measure of the parallelism of an object's sides to the major axis. dimensionless Measure of the symmetry of slope the object's sides in a region near the centroid, dimensionless Measure of the conformity in slope of the object's sides in a region near the centroid, dimensionless Rosenfeld & Kak 1976Harrell et al 1993. also see Fig.…”

Section: The Embryo Classifiermentioning

confidence: 99%

“…also see Fig. 2 Harrell et al 1993 Harrell et al 1993Harrell et al 1993Harrell et al 1993Cazzulino et al 1990Harrell et al 1993Harrell et al 1993. also see […”

Section: The Embryo Classifiermentioning

confidence: 99%

“…Hood & Harrell (in press) described an embryo sorter that was designed to overcome this problem by maintaining the harvested embryos and remnant object in aseptic, in vitro conditions throughout the harvesting process. The operating principles of this device were demonstrated in Harrell et al (1993). However automatic, aseptic harvest was not demonstrated since the system processed embryos fixed with bleach and manually introduced into its pickup device.…”

Section: Introductionmentioning

confidence: 99%

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Automated, in vitro harvest of somatic embryos

Harrell

Bieniek

Hood

et al. 1994

Plant Cell Tiss Organ Cult

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This work has demonstrated the aseptic, automated harvest of somatic embryos from a bioreactor suspension culture. Machine vision, emulating the selection criteria of an experienced biologist, classified embryos as harvestable or non-harvestable as they flowed through a 3 mm glass conduit. Embryos classified as harvestable were separated in a sealed harvest chamber. The system harvested 60% of the embryos at a rate of 2.4 embryos/h and incorrectly harvested less than 1% of the non-harvest objects. The low harvest rate precludes the applicability of this technique to research and commercial tissue culture laboratories. The suspension feed-rate, culture population density and culture homogeneity were identified as the most important factors influencing embryo harvest rate. The performance of this technique on more densely populated cultures was projected using anticipated improvements in suspension feedrate. It was concluded that, under the conditions of this analysis, the harvester would be of limited value in a commercial propagation environment but could be beneficial to many research labs working with plant somatic embryos.

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Section: The Embryo Classifiermentioning

confidence: 99%

Section: The Embryo Classifiermentioning

confidence: 99%

“…also see Fig. 2 Harrell et al 1993 Harrell et al 1993Harrell et al 1993Harrell et al 1993Cazzulino et al 1990Harrell et al 1993Harrell et al 1993. also see […”

Section: The Embryo Classifiermentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Automated, in vitro harvest of somatic embryos

Harrell

Bieniek

Hood

et al. 1994

Plant Cell Tiss Organ Cult

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“…Otherwise, no environmental control systems, for example, can control tissue culture processes automatically. Recent research has revealed that fuzzy control theory, neural network systems and genetic (heuristic and evolutionary) algorithms are useful for selection of quality transplants and somatic embryos and for controlling the environment (Davis 1991;Harrell et al 1993;Kurata 1992, 1994Norimoto and Hasimoto 1991;Norimoto et al 1993).…”

Section: Introductionmentioning

confidence: 97%

Mechanical engineering approaches to plant biotechnology

Miwa

Kushihashi

Kozai

1995

Automation and Environmental Control in Plant Tissue Culture

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Sweet Potato

Dhir

Singh

2008

Compendium of Transgenic Crop Plants

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The sweet potato, Ipomoea batatas (L.) Lam., is an important root crop supporting millions of people throughout the tropical regions of the world and recognized as world's seventh most important food crop following wheat, rice, maize, potato, barley, and cassava. Sweet potato can produce more edible energy per hectare per day than wheat, rice, or cassava, and possesses potent antioxidant, antidiabetic, and anti‐inflammatory properties while use of starch and other processed goods (e.g., alcohol, etc.) is more localized, and increasingly provides an additional source of cash for poor. Despite the fact that tremendous improvements have been made in sweet potatoes, very little progress has been made in terms of yield when compared to other crops. The major challenges conventional breeding faces are the existence of extensive inherent genetic variability, presence of cross incompatible genes, and low seed set rates. Genetic transformation using tissue culture/biotechnology is an alternative that is looked on to as a promising tool to achieve such goals. Various gene delivery systems have been used to develop transgenic sweet potato concentrating on a specific trait/character, which includes Agrobacterium ‐mediated transformation, electroporation, and biolistic approaches. However, the success of transgenic effort can only be achieved by optimizing a successful regeneration protocol and careful consideration of the likely expected effects of transgenic plants on biodiversity of plants, insects, and humans.

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Machine vision based analysis and harvest of somatic embryos

Cited by 14 publications

References 1 publication

Automated, in vitro harvest of somatic embryos

Automated, in vitro harvest of somatic embryos

Mechanical engineering approaches to plant biotechnology

Sweet Potato

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