Macelignan protects HepG2 cells against tert‐butylhydroperoxide‐induced oxidative damage

Abstract

In this study, we investigated the protective effect of macelignan, isolated from Myristica fragrans Houtt. (nutmeg) against tert-butylhydroperoxide (t-BHP)-induced cytotoxicity in a human hepatoma cell line, HepG2. The tetrazolium dye colorimetric test (MTT test) and lactate dehydrogenase (LDH) assay were used to monitor cell viability and necrosis, respectively. Lipid peroxidation [malondialdehyde (MDA) formation] was estimated by the fluorometric method. Intracellular reactive oxygen species (ROS) formation was measured using a fluorescent probe 2',7'-dichlorofluorescein diacetate (DCFH-DA), and DNA damage was detected using single cell gel electrophoresis (comet assay). The results showed that macelignan significantly reduced the cell growth inhibition and necrosis caused by t-BHP. Furthermore, macelignan ameliorated lipid peroxidation as demonstrated by a reduction in MDA formation in a dose-dependent manner. It was also found that macelignan reduced intracellular ROS formation and DNA damaging effect caused by t-BHP. These results strongly suggest that macelignan has significant protective ability against oxidative damage caused by reactive intermediates.

Inhibitory Effects of Macelignan Isolated from Myristica fragrans HOUTT. on Melanin Biosynthesis

“…Many compounds and derivatives such as a-tocopherol, niacinamide, oxyresveratrol, ascorbic acid, and lipoic acid can inhibit melanin synthesis. [34][35][36][37][38] Sohn et al 21) reported that macelig- nan had high free radical-scavenging activity and inhibitory effects on lipid peroxidation induced by tert-butylhydroperoxide. Therefore its melanogenesis-inhibitory activity is thought to be largely due to its antioxidant effects as well as direct inhibition of tyrosinase.…”

“…[13][14][15] Macelignan isolated from M. fragrans belongs to the lignan constituents, 16) and has been reported to have a broad spectrum of biological activities such as antioxidant, antiinflammatory, anticarinogenic, and hepatoprotective effects and cause alteration in hepatic enzyme activities. [17][18][19][20][21] However, the effects of macelignan on melanogenesis have not been examined so far. In this study, we evaluated the inhibitory activity of macelignan on melanin biosynthesis and melanogenic protein expression using melan-a murine melanocytes.…”

Protective Effects of the Hydroalcoholic Extract of Capparis spinosa L. Against Cyclophosphamide-Induced Nephrotoxicity in Mice

“…Therefore, understanding the role played by antioxidants agents during drug-mediated toxicity is important to determine if they have a protective effect against oxidative stress induced by reactive intermediates (41,42).…”

Macelignan Inhibits Melanosome Transfer Mediated by Protease-Activated Receptor-2 in Keratinocytes

“…(nutmeg), exerts anti-oxidant, anti-inflammatory, anti-carcinogenic, anti-diabetic, and hepatoprotective effects. [15][16][17] In addition, macelignan has been reported to inhibit melanin biosynthesis in murine melan-a melanocytes. 18) However, the effects of macelignan on melanosome transfer have not been determined.…”