M2 Macrophage-Derived Concentrated Conditioned Media Significantly Improves Skin Wound Healing

Savitri, Cininta; Kwon, Jae Won; Drobyshava, Valeryia; Ha, Sang Su

doi:10.1007/s13770-021-00414-4

Cited by 10 publications

(9 citation statements)

References 35 publications

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“…Previously, Vural et al found that HIF stimulated VEGF, promoted angiogenesis, and accelerated wound healing 196 . M2-polarized macrophages mediate wound healing through Akt, ERK1/2, and STAT3 pathway 197 – 200 , and the promoting effect of lactate on the polarization and function of M2 macrophages has been discussed.…”

Section: Roles Of Lactic Acid In Pathophysiological Processesmentioning

confidence: 99%

Lactate metabolism in human health and disease

Yang

Zhang

et al. 2022

Sig Transduct Target Ther

380

234

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The current understanding of lactate extends from its origins as a byproduct of glycolysis to its role in tumor metabolism, as identified by studies on the Warburg effect. The lactate shuttle hypothesis suggests that lactate plays an important role as a bridging signaling molecule that coordinates signaling among different cells, organs and tissues. Lactylation is a posttranslational modification initially reported by Professor Yingming Zhao’s research group in 2019. Subsequent studies confirmed that lactylation is a vital component of lactate function and is involved in tumor proliferation, neural excitation, inflammation and other biological processes. An indispensable substance for various physiological cellular functions, lactate plays a regulatory role in different aspects of energy metabolism and signal transduction. Therefore, a comprehensive review and summary of lactate is presented to clarify the role of lactate in disease and to provide a reference and direction for future research. This review offers a systematic overview of lactate homeostasis and its roles in physiological and pathological processes, as well as a comprehensive overview of the effects of lactylation in various diseases, particularly inflammation and cancer.

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Section: Roles Of Lactic Acid In Pathophysiological Processesmentioning

confidence: 99%

Lactate metabolism in human health and disease

Yang

Zhang

et al. 2022

Sig Transduct Target Ther

380

234

View full text Add to dashboard Cite

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“…These results were supported by qRT‐PCR analysis of wound tissues (Figure 5e). Collagen type 1, a key component of the extracellular matrix, is important during proliferative and remodeling phases of wound healing 92–94 . Significant upregulation of involucrin and collagen type 1 was identified in the +RLT group compared to NT and –RLT groups.…”

Section: Resultsmentioning

confidence: 99%

Enhancing viability and angiogenic efficacy of mesenchymal stem cells via HSP90α and HSP27 regulation based on ROS stimulation for wound healing

Seo

Kim

Hyun

et al. 2023

Bioengineering & Transla Med

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Light‐based therapy has been reported as a potential preconditioning strategy to induce intracellular reactive oxygen species (ROS) signaling and improve the angiogenic properties of various types of cells. However, bio‐stimulation mechanisms of light therapy in terms of ROS‐heat shock proteins (HSPs) mediated anti‐apoptotic and angiogenic pathways in human adult stem cells have not been fully delineated yet. Commonly used light sources such as light‐emitting diode (LED) and laser are accompanied by drawbacks, such as phototoxicity, thermal damage, and excessive ROS induction, so the role and clinical implications of light‐induced HSPs need to be investigated using a heat‐independent light source. Here, we introduced organic LED (OLED) at 610 nm wavelength as a new light source to prevent thermal effects from interfering with the expression of HSPs. Our results showed that light therapy using OLED significantly upregulated anti‐apoptotic and angiogenic factors in human bone marrow mesenchymal stem cells (hMSCs) at both gene and protein levels via the activation of HSP90α and HSP27, which were stimulated by ROS. In a mouse wound‐closing model, rapid recovery and improved re‐epithelization were observed in the light‐treated hMSCs transplant group. This study demonstrates that the upregulation of Akt (protein kinase B)‐nuclear factor kappa‐light‐chain‐enhancer of activated B cells (NF‐κB) signaling, caused by HSP90α and HSP27 expression, is the mechanism behind the anti‐apoptotic and angiogenic effects of OLED treatment on stem cells.

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“…CCL5 may participate in tissue regeneration by recruiting stroma cells via CCR1 ( 109 ). Moreover, CCL5 released by M2 macrophages may improve skin wound healing ( 110 ). In contrast, the increased production of CCL5 together with type I interferon after fungal infection may be responsible for impaired mucosal healing in Crohn’s disease patients and in mice ( 111 ).…”

Section: An Example Of Pro-resolving Factors Released By Macrophages ...mentioning

confidence: 99%

Resolution therapy: Harnessing efferocytic macrophages to trigger the resolution of inflammation

Saas

Vetter²,

Maraux³

et al. 2022

Front. Immunol.

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Several chronic inflammatory diseases are associated with non-resolving inflammation. Conventional anti-inflammatory drugs fail to completely cure these diseases. Resolution pharmacology is a new therapeutic approach based on the use of pro-resolving mediators that accelerate the resolution phase of inflammation by targeting the productive phase of inflammation. Indeed, pro-resolving mediators prevent leukocyte recruitment and induce apoptosis of accumulated leukocytes. This approach is now called resolution therapy with the introduction of complex biological drugs and cell-based therapies. The main objective of resolution therapy is to specifically reduce the duration of the resolution phase to accelerate the return to homeostasis. Under physiological conditions, macrophages play a critical role in the resolution of inflammation. Indeed, after the removal of apoptotic cells (a process called efferocytosis), macrophages display anti-inflammatory reprogramming and subsequently secrete multiple pro-resolving factors. These factors can be used as resolution therapy. Here, we review the different mechanisms leading to anti-inflammatory reprogramming of macrophages after efferocytosis and the pro-resolving factors released by these efferocytic macrophages. We classify these mechanisms in three different categories: macrophage reprogramming induced by apoptotic cell-derived factors, by molecules expressed by apoptotic cells (i.e., “eat-me” signals), and induced by the digestion of apoptotic cell-derived materials. We also evoke that macrophage reprogramming may result from cooperative mechanisms, for instance, implicating the apoptotic cell-induced microenvironment (including cellular metabolites, specific cytokines or immune cells). Then, we describe a new drug candidate belonging to this resolution therapy. This candidate, called SuperMApo, corresponds to the secretome of efferocytic macrophages. We discuss its production, the pro-resolving factors present in this drug, as well as the results obtained in experimental models of chronic (e.g., arthritis, colitis) and acute (e.g., peritonitis or xenogeneic graft-versus-host disease) inflammatory diseases.

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M2 Macrophage-Derived Concentrated Conditioned Media Significantly Improves Skin Wound Healing

Cited by 10 publications

References 35 publications

Lactate metabolism in human health and disease

Lactate metabolism in human health and disease

Enhancing viability and angiogenic efficacy of mesenchymal stem cells via HSP90α and HSP27 regulation based on ROS stimulation for wound healing

Resolution therapy: Harnessing efferocytic macrophages to trigger the resolution of inflammation

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